Quercus Cerris Extracts Limit Staphylococcus Aureus Biofilm Formation

Quercus Cerris Extracts Limit Staphylococcus Aureus Biofilm Formation. Hobby, G. H., Quave, C. L., Nelson, K., Compadre, C. M., Beenken, K. E., & Smeltzer, M. S. 144(3):812–815.

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Quercus cerris L., Fagaceae has been used in traditional Mediterranean medicine for numerous purposes, including anti-infective therapies for diarrhea and wound care. To evaluate the anti-staphylococcal activity of fractions of ethanolic extracts of Q. cerris leaf and stem/fruit samples in models for biofilm and growth inhibition. Ethanolic extracts of Q. cerris leaves and stems/fruits were prepared, resuspended in water and fractioned by successively partitioning with hexane, ethyl acetate and butanol. The ability of the fractions to inhibit Staphylococcus aureus biofilm formation was tested using static crystal violet staining methods and confocal laser scanning microscopy. Growth studies were conducted to determine if the diminished capacity to form a biofilm was related to growth inhibition. The butanol extracts of both the leaf and stem/fruit samples were the most active, and at a dose of 200~Î¼g/ml, the capacity to form a biofilm was limited to a level equivalent to that of the sarA mutant controls. Further examination of the impact of these fractions on S. aureus growth revealed that biofilm inhibition by the leaf butanol fraction was due to its bacteriostatic activity. The stem/fruit butanol fraction, however, showed a limited impact on growth, thus demonstrating that biofilm inhibition in this case is not related to the bacteriostatic activity of the extract. Our evaluation of a medicinal plant used in Mediterranean ethnotherapies for infectious disease has demonstrated significant activity in the inhibition of staphylococcal biofilm formation with a mechanism unrelated to staphylococcal growth inhibition. These results contribute towards validation of this botanical remedy and form the groundwork for future studies in the search for novel biofilm inhibiting drugs.

@article{hobbyQuercusCerrisExtracts2012,
  title = {Quercus Cerris Extracts Limit {{Staphylococcus}} Aureus Biofilm Formation},
  author = {Hobby, Gerren H. and Quave, Cassandra L. and Nelson, Katie and Compadre, Cesar M. and Beenken, Karen E. and Smeltzer, Mark S.},
  date = {2012-12},
  journaltitle = {Journal of Ethnopharmacology},
  volume = {144},
  pages = {812--815},
  issn = {0378-8741},
  doi = {10.1016/j.jep.2012.10.042},
  url = {https://doi.org/10.1016/j.jep.2012.10.042},
  abstract = {Quercus cerris L., Fagaceae has been used in traditional Mediterranean medicine for numerous purposes, including anti-infective therapies for diarrhea and wound care. To evaluate the anti-staphylococcal activity of fractions of ethanolic extracts of Q. cerris leaf and stem/fruit samples in models for biofilm and growth inhibition. Ethanolic extracts of Q. cerris leaves and stems/fruits were prepared, resuspended in water and fractioned by successively partitioning with hexane, ethyl acetate and butanol. The ability of the fractions to inhibit Staphylococcus aureus biofilm formation was tested using static crystal violet staining methods and confocal laser scanning microscopy. Growth studies were conducted to determine if the diminished capacity to form a biofilm was related to growth inhibition. The butanol extracts of both the leaf and stem/fruit samples were the most active, and at a dose of 200~Î¼g/ml, the capacity to form a biofilm was limited to a level equivalent to that of the sarA mutant controls. Further examination of the impact of these fractions on S. aureus growth revealed that biofilm inhibition by the leaf butanol fraction was due to its bacteriostatic activity. The stem/fruit butanol fraction, however, showed a limited impact on growth, thus demonstrating that biofilm inhibition in this case is not related to the bacteriostatic activity of the extract. Our evaluation of a medicinal plant used in Mediterranean ethnotherapies for infectious disease has demonstrated significant activity in the inhibition of staphylococcal biofilm formation with a mechanism unrelated to staphylococcal growth inhibition. These results contribute towards validation of this botanical remedy and form the groundwork for future studies in the search for novel biofilm inhibiting drugs.},
  keywords = {*imported-from-citeulike-INRMM,~INRMM-MiD:c-11619286,biofilm,quercus-cerris,quercus-spp,staphylococcus-aureus},
  number = {3}
}

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Further examination of the impact of these fractions on S. aureus growth revealed that biofilm inhibition by the leaf butanol fraction was due to its bacteriostatic activity. The stem/fruit butanol fraction, however, showed a limited impact on growth, thus demonstrating that biofilm inhibition in this case is not related to the bacteriostatic activity of the extract. Our evaluation of a medicinal plant used in Mediterranean ethnotherapies for infectious disease has demonstrated significant activity in the inhibition of staphylococcal biofilm formation with a mechanism unrelated to staphylococcal growth inhibition. 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