Attachment to laminin-111 facilitates transforming growth factor beta-induced expression of matrix metalloproteinase-3 in synovial fibroblasts. Hoberg, M., Rudert, M., Pap, T., Klein, G., Gay, S., & Aicher, W. K. Annals of the rheumatic diseases, 66(4):446–451, April, 2007. doi abstract bibtex BACKGROUND: In the synovial membrane of patients with rheumatoid arthritis (RA), a strong expression of laminins and matrix degrading proteases was reported. AIM: To investigate the regulation of matrix metalloproteinases (MMPs) in synovial fibroblasts (SFs) of patients with osteoarthritis (OA) and RA by attachment to laminin-1 (LM-111) and in the presence or absence of costimulatory signals provided by transforming growth factor beta (TGFbeta). METHODS: SFs were seeded in laminin-coated flasks and activated by addition of TGFbeta. The expression of genes was investigated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), immunocytochemistry and ELISA, and intracellular signalling pathways by immunoblotting, and by poisoning p38MAPK by SB203580, MEK-ERK by PD98059 and SMAD2 by A-83-01. RESULTS: Attachment of SF to LM-111 did not activate the expression of MMPs, but addition of TGFbeta induced a fivefold higher expression of MMP-3. Incubation of SF on LM-111 in the presence of TGFbeta induced a significant 12-fold higher expression of MMP-3 mRNA, and secretion of
@article{hoberg_attachment_2007,
title = {Attachment to laminin-111 facilitates transforming growth factor beta-induced expression of matrix metalloproteinase-3 in synovial fibroblasts.},
volume = {66},
doi = {10.1136/ard.2006.060228},
abstract = {BACKGROUND: In the synovial membrane of patients with rheumatoid arthritis (RA), a strong expression of laminins and matrix degrading proteases was reported. AIM: To investigate the regulation of matrix metalloproteinases (MMPs) in synovial fibroblasts (SFs) of patients with osteoarthritis (OA) and RA by attachment to laminin-1 (LM-111) and in the presence or absence of costimulatory signals provided by transforming growth factor beta (TGFbeta). METHODS: SFs were seeded in laminin-coated flasks and activated by addition of TGFbeta. The expression of genes was investigated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), immunocytochemistry and ELISA, and intracellular signalling pathways by immunoblotting, and by poisoning p38MAPK by SB203580, MEK-ERK by PD98059 and SMAD2 by A-83-01. RESULTS: Attachment of SF to LM-111 did not activate the expression of MMPs, but addition of TGFbeta induced a fivefold higher expression of MMP-3. Incubation of SF on LM-111 in the presence of TGFbeta induced a significant 12-fold higher expression of MMP-3 mRNA, and secretion of},
language = {eng},
number = {4},
journal = {Annals of the rheumatic diseases},
author = {Hoberg, Maik and Rudert, Maximilian and Pap, Thomas and Klein, Gerd and Gay, Steffen and Aicher, Wilhelm K.},
month = apr,
year = {2007},
pmid = {17124250},
pmcid = {PMC1856036},
keywords = {Aged, Arthritis, Rheumatoid/*enzymology/metabolism/pathology, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases/metabolism, Female, Fibroblasts/enzymology, Gene Expression Regulation, Enzymologic/drug effects, Humans, Laminin/*metabolism, Male, Matrix Metalloproteinase 3/genetics/*metabolism, Middle Aged, Phosphorylation, Recombinant Proteins/pharmacology, Reverse Transcriptase Polymerase Chain Reaction/methods, Signal Transduction, Synovial Membrane/*enzymology/pathology, Transforming Growth Factor beta/*pharmacology},
pages = {446--451},
}
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AIM: To investigate the regulation of matrix metalloproteinases (MMPs) in synovial fibroblasts (SFs) of patients with osteoarthritis (OA) and RA by attachment to laminin-1 (LM-111) and in the presence or absence of costimulatory signals provided by transforming growth factor beta (TGFbeta). METHODS: SFs were seeded in laminin-coated flasks and activated by addition of TGFbeta. The expression of genes was investigated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), immunocytochemistry and ELISA, and intracellular signalling pathways by immunoblotting, and by poisoning p38MAPK by SB203580, MEK-ERK by PD98059 and SMAD2 by A-83-01. RESULTS: Attachment of SF to LM-111 did not activate the expression of MMPs, but addition of TGFbeta induced a fivefold higher expression of MMP-3. Incubation of SF on LM-111 in the presence of TGFbeta induced a significant 12-fold higher expression of MMP-3 mRNA, and secretion of","language":"eng","number":"4","journal":"Annals of the rheumatic diseases","author":[{"propositions":[],"lastnames":["Hoberg"],"firstnames":["Maik"],"suffixes":[]},{"propositions":[],"lastnames":["Rudert"],"firstnames":["Maximilian"],"suffixes":[]},{"propositions":[],"lastnames":["Pap"],"firstnames":["Thomas"],"suffixes":[]},{"propositions":[],"lastnames":["Klein"],"firstnames":["Gerd"],"suffixes":[]},{"propositions":[],"lastnames":["Gay"],"firstnames":["Steffen"],"suffixes":[]},{"propositions":[],"lastnames":["Aicher"],"firstnames":["Wilhelm","K."],"suffixes":[]}],"month":"April","year":"2007","pmid":"17124250","pmcid":"PMC1856036","keywords":"Aged, Arthritis, Rheumatoid/*enzymology/metabolism/pathology, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases/metabolism, Female, Fibroblasts/enzymology, Gene Expression Regulation, Enzymologic/drug effects, Humans, Laminin/*metabolism, Male, Matrix Metalloproteinase 3/genetics/*metabolism, Middle Aged, Phosphorylation, Recombinant Proteins/pharmacology, Reverse Transcriptase Polymerase Chain Reaction/methods, Signal Transduction, Synovial Membrane/*enzymology/pathology, Transforming Growth Factor beta/*pharmacology","pages":"446–451","bibtex":"@article{hoberg_attachment_2007,\n\ttitle = {Attachment to laminin-111 facilitates transforming growth factor beta-induced expression of matrix metalloproteinase-3 in synovial fibroblasts.},\n\tvolume = {66},\n\tdoi = {10.1136/ard.2006.060228},\n\tabstract = {BACKGROUND: In the synovial membrane of patients with rheumatoid arthritis (RA), a strong expression of laminins and matrix degrading proteases was reported. AIM: To investigate the regulation of matrix metalloproteinases (MMPs) in synovial fibroblasts (SFs) of patients with osteoarthritis (OA) and RA by attachment to laminin-1 (LM-111) and in the presence or absence of costimulatory signals provided by transforming growth factor beta (TGFbeta). METHODS: SFs were seeded in laminin-coated flasks and activated by addition of TGFbeta. The expression of genes was investigated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), immunocytochemistry and ELISA, and intracellular signalling pathways by immunoblotting, and by poisoning p38MAPK by SB203580, MEK-ERK by PD98059 and SMAD2 by A-83-01. RESULTS: Attachment of SF to LM-111 did not activate the expression of MMPs, but addition of TGFbeta induced a fivefold higher expression of MMP-3. Incubation of SF on LM-111 in the presence of TGFbeta induced a significant 12-fold higher expression of MMP-3 mRNA, and secretion of},\n\tlanguage = {eng},\n\tnumber = {4},\n\tjournal = {Annals of the rheumatic diseases},\n\tauthor = {Hoberg, Maik and Rudert, Maximilian and Pap, Thomas and Klein, Gerd and Gay, Steffen and Aicher, Wilhelm K.},\n\tmonth = apr,\n\tyear = {2007},\n\tpmid = {17124250},\n\tpmcid = {PMC1856036},\n\tkeywords = {Aged, Arthritis, Rheumatoid/*enzymology/metabolism/pathology, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases/metabolism, Female, Fibroblasts/enzymology, Gene Expression Regulation, Enzymologic/drug effects, Humans, Laminin/*metabolism, Male, Matrix Metalloproteinase 3/genetics/*metabolism, Middle Aged, Phosphorylation, Recombinant Proteins/pharmacology, Reverse Transcriptase Polymerase Chain Reaction/methods, Signal Transduction, Synovial Membrane/*enzymology/pathology, Transforming Growth Factor beta/*pharmacology},\n\tpages = {446--451},\n}\n\n","author_short":["Hoberg, M.","Rudert, M.","Pap, T.","Klein, G.","Gay, S.","Aicher, W. 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