Induction of endostatin expression in meniscal fibrochondrocytes by co-culture with endothelial cells. Hoberg, M., Schmidt, E. L., Tuerk, M., Stark, V., Aicher, W. K., & Rudert, M. Archives of orthopaedic and trauma surgery, 129(8):1137–1143, August, 2009. doi abstract bibtex INTRODUCTION: The ultimate goal after meniscus damage is the preservation of the original meniscal tissue, which is often impossible due to the limited healing capacity of meniscal lesions, especially in the avascular zone. Factors produced by endothelial cells of meniscal vessels may contribute to better wound healing in vascularized zones. We therefore investigated the expression of different angiogenic factors, growth hormones and cytokines in human fibrochondrocytes and in fibrochondrocytes upon co-culture with endothelial cells, to examine mechanisms of repair of meniscal injury in more detail and to investigate the potential use of endothelial cells in co-cultures for autologous meniscal repair utilizing tissue engineering technology. MATERIALS AND METHODS: Gene expression of SMAD-4, iNOS, IL-1beta, VEGF, MMP-1, MMP-3, MMP-13, aggrecan, biglycan, vimentin, collagen-I, -II, -III, -IV, -VI, -X, -XVIII, angiopoietin-1, angiopoietin-2, and thrombostatin-1 were investigated in fibrochondrocytes in comparison to cells in co-culture with human umbilical vein endothelial cells (HUVEC). The expression of endostatin was enumerated in cell supernatants. A proliferation assay was used to investigate the mitotic activity of the cells. RESULTS: In presence of HUVEC, meniscal fibrochondrocytes expressed SMAD-4, iNOS,
@article{hoberg_induction_2009,
title = {Induction of endostatin expression in meniscal fibrochondrocytes by co-culture with endothelial cells.},
volume = {129},
doi = {10.1007/s00402-008-0766-8},
abstract = {INTRODUCTION: The ultimate goal after meniscus damage is the preservation of the original meniscal tissue, which is often impossible due to the limited healing capacity of meniscal lesions, especially in the avascular zone. Factors produced by endothelial cells of meniscal vessels may contribute to better wound healing in vascularized zones. We therefore investigated the expression of different angiogenic factors, growth hormones and cytokines in human fibrochondrocytes and in fibrochondrocytes upon co-culture with endothelial cells, to examine mechanisms of repair of meniscal injury in more detail and to investigate the potential use of endothelial cells in co-cultures for autologous meniscal repair utilizing tissue engineering technology. MATERIALS AND METHODS: Gene expression of SMAD-4, iNOS, IL-1beta, VEGF, MMP-1, MMP-3, MMP-13, aggrecan, biglycan, vimentin, collagen-I, -II, -III, -IV, -VI, -X, -XVIII, angiopoietin-1, angiopoietin-2, and thrombostatin-1 were investigated in fibrochondrocytes in comparison to cells in co-culture with human umbilical vein endothelial cells (HUVEC). The expression of endostatin was enumerated in cell supernatants. A proliferation assay was used to investigate the mitotic activity of the cells. RESULTS: In presence of HUVEC, meniscal fibrochondrocytes expressed SMAD-4, iNOS,},
language = {eng},
number = {8},
journal = {Archives of orthopaedic and trauma surgery},
author = {Hoberg, Maik and Schmidt, Edda L. and Tuerk, Melanie and Stark, Viviane and Aicher, Wilhelm K. and Rudert, Maximilian},
month = aug,
year = {2009},
pmid = {18839188},
keywords = {Cell Proliferation, Cells, Cultured, Chondrocytes/*metabolism, Endostatins/*biosynthesis, Endothelial Cells, Humans, Menisci, Tibial/metabolism/*physiology, Regeneration/*physiology, Umbilical Veins/cytology},
pages = {1137--1143},
}
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We therefore investigated the expression of different angiogenic factors, growth hormones and cytokines in human fibrochondrocytes and in fibrochondrocytes upon co-culture with endothelial cells, to examine mechanisms of repair of meniscal injury in more detail and to investigate the potential use of endothelial cells in co-cultures for autologous meniscal repair utilizing tissue engineering technology. MATERIALS AND METHODS: Gene expression of SMAD-4, iNOS, IL-1beta, VEGF, MMP-1, MMP-3, MMP-13, aggrecan, biglycan, vimentin, collagen-I, -II, -III, -IV, -VI, -X, -XVIII, angiopoietin-1, angiopoietin-2, and thrombostatin-1 were investigated in fibrochondrocytes in comparison to cells in co-culture with human umbilical vein endothelial cells (HUVEC). The expression of endostatin was enumerated in cell supernatants. A proliferation assay was used to investigate the mitotic activity of the cells. RESULTS: In presence of HUVEC, meniscal fibrochondrocytes expressed SMAD-4, iNOS,","language":"eng","number":"8","journal":"Archives of orthopaedic and trauma surgery","author":[{"propositions":[],"lastnames":["Hoberg"],"firstnames":["Maik"],"suffixes":[]},{"propositions":[],"lastnames":["Schmidt"],"firstnames":["Edda","L."],"suffixes":[]},{"propositions":[],"lastnames":["Tuerk"],"firstnames":["Melanie"],"suffixes":[]},{"propositions":[],"lastnames":["Stark"],"firstnames":["Viviane"],"suffixes":[]},{"propositions":[],"lastnames":["Aicher"],"firstnames":["Wilhelm","K."],"suffixes":[]},{"propositions":[],"lastnames":["Rudert"],"firstnames":["Maximilian"],"suffixes":[]}],"month":"August","year":"2009","pmid":"18839188","keywords":"Cell Proliferation, Cells, Cultured, Chondrocytes/*metabolism, Endostatins/*biosynthesis, Endothelial Cells, Humans, Menisci, Tibial/metabolism/*physiology, Regeneration/*physiology, Umbilical Veins/cytology","pages":"1137–1143","bibtex":"@article{hoberg_induction_2009,\n\ttitle = {Induction of endostatin expression in meniscal fibrochondrocytes by co-culture with endothelial cells.},\n\tvolume = {129},\n\tdoi = {10.1007/s00402-008-0766-8},\n\tabstract = {INTRODUCTION: The ultimate goal after meniscus damage is the preservation of the original meniscal tissue, which is often impossible due to the limited healing capacity of meniscal lesions, especially in the avascular zone. Factors produced by endothelial cells of meniscal vessels may contribute to better wound healing in vascularized zones. We therefore investigated the expression of different angiogenic factors, growth hormones and cytokines in human fibrochondrocytes and in fibrochondrocytes upon co-culture with endothelial cells, to examine mechanisms of repair of meniscal injury in more detail and to investigate the potential use of endothelial cells in co-cultures for autologous meniscal repair utilizing tissue engineering technology. MATERIALS AND METHODS: Gene expression of SMAD-4, iNOS, IL-1beta, VEGF, MMP-1, MMP-3, MMP-13, aggrecan, biglycan, vimentin, collagen-I, -II, -III, -IV, -VI, -X, -XVIII, angiopoietin-1, angiopoietin-2, and thrombostatin-1 were investigated in fibrochondrocytes in comparison to cells in co-culture with human umbilical vein endothelial cells (HUVEC). The expression of endostatin was enumerated in cell supernatants. 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