Double-Stranded Ligation Assay for the Rapid Multiplex Quantification of MicroRNAs. Hofmann, S., Huang, Y., Paulicka, P., Kappel, A., Katus, H. A, Keller, A., Meder, B., Stähler, C. F., & Gumbrecht, W. Analytical chemistry, 87:12104–12111, December, 2015. doi abstract bibtex MicroRNAs are auspicious candidates for a new generation of biomarkers. The detection of microRNA panels in body fluids promises early diagnosis of many diseases, including cancer or acute coronary syndrome. For a fast, sensitive, and specific detection of microRNA panels on the bedside, medical point-of-care systems that measure those biomarkers are required. As microchips are promising technical tools for a robust signal measurement at biochemical interfaces, we developed an assay for the electrochemical multiplex quantification of microRNAs on a CMOS chip with interdigitated gold electrode sensor positions. The method is based on the formation of a tripartite hybridization complex and subsequent both-sided ligation of the target nucleic acid to a reporter and capture strand. With a time to results of 30 min, the reported assay achieves a limit of detection below 1 pM, at a specificity down to single mismatch discrimination. It also offers very good signal dynamics between 1 pM and 1 nM, thus, allowing reliable quantification of the detected microRNAs and easy implementation into automated devices due to a simple workflow.
@Article{Hofmann2015,
author = {Hofmann, Stefan and Huang, Yiwei and Paulicka, Peter and Kappel, Andreas and Katus, Hugo A and Keller, Andreas and Meder, Benjamin and Stähler, Cord Friedrich and Gumbrecht, Walter},
title = {Double-Stranded Ligation Assay for the Rapid Multiplex Quantification of MicroRNAs.},
journal = {Analytical chemistry},
year = {2015},
volume = {87},
pages = {12104--12111},
month = dec,
issn = {1520-6882},
abstract = {MicroRNAs are auspicious candidates for a new generation of biomarkers. The detection of microRNA panels in body fluids promises early diagnosis of many diseases, including cancer or acute coronary syndrome. For a fast, sensitive, and specific detection of microRNA panels on the bedside, medical point-of-care systems that measure those biomarkers are required. As microchips are promising technical tools for a robust signal measurement at biochemical interfaces, we developed an assay for the electrochemical multiplex quantification of microRNAs on a CMOS chip with interdigitated gold electrode sensor positions. The method is based on the formation of a tripartite hybridization complex and subsequent both-sided ligation of the target nucleic acid to a reporter and capture strand. With a time to results of 30 min, the reported assay achieves a limit of detection below 1 pM, at a specificity down to single mismatch discrimination. It also offers very good signal dynamics between 1 pM and 1 nM, thus, allowing reliable quantification of the detected microRNAs and easy implementation into automated devices due to a simple workflow. },
chemicals = {MicroRNAs},
citation-subset = {IM},
completed = {2016-09-01},
country = {United States},
doi = {10.1021/acs.analchem.5b02850},
issn-linking = {0003-2700},
issue = {24},
keywords = {Genetic Techniques; Limit of Detection; MicroRNAs, analysis, genetics; Molecular Diagnostic Techniques; Time Factors},
nlm-id = {0370536},
owner = {NLM},
pmid = {26574152},
pubmodel = {Print-Electronic},
pubstatus = {ppublish},
revised = {2015-12-15},
}
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