Contribution of Amino Acid Catabolism to the Tissue Specific Persistence of Campylobacter jejuni in a Murine Colonization Model. Hofreuter, D., Mohr, J., Wensel, O., Rademacher, S., Schreiber, K., Schomburg, D., Gao, B., & Galán, J. E. PLoS ONE, 7(11):e50699, Public Library of Science, 11, 2012. ![Contribution of Amino Acid Catabolism to the Tissue Specific Persistence of Campylobacter jejuni in a Murine Colonization Model [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Campylobacter jejuni is a major cause of food-borne disease in industrialized countries. Carbohydrate utilization by C. jejuni is severely restricted, and knowledge about which substrates fuel C. jejuni infection and growth is limited. Some amino acids have been shown to serve as carbon sources both in vitro and in vivo. In the present study we investigated the contribution of serine and proline catabolism to the in vitro and in vivo growth of C. jejuni 81-176. We confirmed that the serine transporter SdaC and the serine ammonia-lyase SdaA are required for serine utilization, and demonstrated that a predicted proline permease PutP and a bifunctional proline/delta-1-pyrroline-5-carboxylate dehydrogenase PutA are required for proline utilization by C. jejuni 81-176. C. jejuni 81-176 mutants unable to utilize serine were shown to be severely defective for colonization of the intestine and systemic tissues in a mouse model of infection. In contrast, C. jejuni 81-176 mutants unable to utilize proline were only defective for intestinal colonization. These results further emphasize the importance of amino acid utilization in C. jejuni colonization of various tissues.
@article{10.1371/journal.pone.0050699,
abstract = {<p><italic>Campylobacter jejuni</italic> is a major cause of food-borne disease in industrialized countries. Carbohydrate utilization by <italic>C. jejuni</italic> is severely restricted, and knowledge about which substrates fuel <italic>C. jejuni</italic> infection and growth is limited. Some amino acids have been shown to serve as carbon sources both <italic>in vitro</italic> and <italic>in vivo</italic>. In the present study we investigated the contribution of serine and proline catabolism to the <italic>in</italic> <italic>vitro</italic> and <italic>in</italic> <italic>vivo</italic> growth of <italic>C</italic>. <italic>jejuni</italic> 81-176. We confirmed that the serine transporter SdaC and the serine ammonia-lyase SdaA are required for serine utilization, and demonstrated that a predicted proline permease PutP and a bifunctional proline/delta-1-pyrroline-5-carboxylate dehydrogenase PutA are required for proline utilization by <italic>C. jejuni</italic> 81-176. <italic>C. jejuni</italic> 81-176 mutants unable to utilize serine were shown to be severely defective for colonization of the intestine and systemic tissues in a mouse model of infection. In contrast, <italic>C. jejuni</italic> 81-176 mutants unable to utilize proline were only defective for intestinal colonization. These results further emphasize the importance of amino acid utilization in <italic>C. jejuni</italic> colonization of various tissues.</p>},
added-at = {2013-02-27T16:25:34.000+0100},
author = {Hofreuter, Dirk and Mohr, Juliane and Wensel, Olga and Rademacher, Sebastian and Schreiber, Kerstin and Schomburg, Dietmar and Gao, Beile and Galán, Jorge E.},
biburl = {https://www.bibsonomy.org/bibtex/256e7e364843bc0211b233a2ee78f9e2f/l3s},
doi = {10.1371/journal.pone.0050699},
interhash = {ff3cd8fcb87a4f4c76212937932a5cf9},
intrahash = {56e7e364843bc0211b233a2ee78f9e2f},
journal = {PLoS ONE},
keywords = {Campylobacter jejuni Murine Colonization},
month = {11},
number = 11,
pages = {e50699},
publisher = {Public Library of Science},
timestamp = {2013-02-27T16:25:34.000+0100},
title = {Contribution of Amino Acid Catabolism to the Tissue Specific Persistence of Campylobacter jejuni in a Murine Colonization Model},
url = {http://dx.doi.org/10.1371%2Fjournal.pone.0050699},
volume = 7,
year = 2012
}
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