@article{huang_isolation_2004,
	title = {Isolation of outer membrane of {Synechocystis} sp {PCC} 6803 and its proteomic characterization},
	volume = {3},
	issn = {1535-9476},
	doi = {10/dxr94c},
	abstract = {In this report, we describe a newly developed method for isolating outer membranes from Synechocystis sp. PCC 6803 cells. The purity of the outer membrane fraction was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. We investigated the protein composition of the outer membrane using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry followed by database identification. Forty-nine proteins were identified corresponding to 29 different gene products. All of the identified proteins have a putative N-terminal signal peptide. About 40\% of the proteins identified represent hypothetical proteins with unknown function. Among the proteins identified are a Toc75 homologue, a protein that was initially found in the outer envelope of chloroplasts in pea, as well as TolC, putative porins, and a pilus protein. Other proteins identified include ABC transporters and GumB, which has a suggested function in carbohydrate export. A number of proteases such as HtrA were also found in the outer membrane of Synechocystis sp. PCC 6803.},
	language = {English},
	number = {6},
	journal = {Molecular \& Cellular Proteomics},
	author = {Huang, F. and Hedman, E. and Funk, C. and Kieselbach, T. and Schroder, W. P. and Norling, B.},
	month = jun,
	year = {2004},
	note = {Place: Rockville
Publisher: Amer Soc Biochemistry Molecular Biology Inc
WOS:000222029100007},
	keywords = {crystal-structure, escherichia-coli, genetic-analysis, htra family, multidrug efflux, photosystem-ii, plasma-membranes, protein, pseudomonas-aeruginosa, thylakoid membranes},
	pages = {586--595},
}

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Forty-nine proteins were identified corresponding to 29 different gene products. All of the identified proteins have a putative N-terminal signal peptide. About 40% of the proteins identified represent hypothetical proteins with unknown function. Among the proteins identified are a Toc75 homologue, a protein that was initially found in the outer envelope of chloroplasts in pea, as well as TolC, putative porins, and a pilus protein. Other proteins identified include ABC transporters and GumB, which has a suggested function in carbohydrate export. A number of proteases such as HtrA were also found in the outer membrane of Synechocystis sp. PCC 6803.","language":"English","number":"6","journal":"Molecular & Cellular Proteomics","author":[{"propositions":[],"lastnames":["Huang"],"firstnames":["F."],"suffixes":[]},{"propositions":[],"lastnames":["Hedman"],"firstnames":["E."],"suffixes":[]},{"propositions":[],"lastnames":["Funk"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Kieselbach"],"firstnames":["T."],"suffixes":[]},{"propositions":[],"lastnames":["Schroder"],"firstnames":["W.","P."],"suffixes":[]},{"propositions":[],"lastnames":["Norling"],"firstnames":["B."],"suffixes":[]}],"month":"June","year":"2004","note":"Place: Rockville Publisher: Amer Soc Biochemistry Molecular Biology Inc WOS:000222029100007","keywords":"crystal-structure, escherichia-coli, genetic-analysis, htra family, multidrug efflux, photosystem-ii, plasma-membranes, protein, pseudomonas-aeruginosa, thylakoid membranes","pages":"586–595","bibtex":"@article{huang_isolation_2004,\n\ttitle = {Isolation of outer membrane of {Synechocystis} sp {PCC} 6803 and its proteomic characterization},\n\tvolume = {3},\n\tissn = {1535-9476},\n\tdoi = {10/dxr94c},\n\tabstract = {In this report, we describe a newly developed method for isolating outer membranes from Synechocystis sp. PCC 6803 cells. The purity of the outer membrane fraction was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. We investigated the protein composition of the outer membrane using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry followed by database identification. Forty-nine proteins were identified corresponding to 29 different gene products. All of the identified proteins have a putative N-terminal signal peptide. About 40\\% of the proteins identified represent hypothetical proteins with unknown function. Among the proteins identified are a Toc75 homologue, a protein that was initially found in the outer envelope of chloroplasts in pea, as well as TolC, putative porins, and a pilus protein. Other proteins identified include ABC transporters and GumB, which has a suggested function in carbohydrate export. A number of proteases such as HtrA were also found in the outer membrane of Synechocystis sp. PCC 6803.},\n\tlanguage = {English},\n\tnumber = {6},\n\tjournal = {Molecular \\& Cellular Proteomics},\n\tauthor = {Huang, F. and Hedman, E. and Funk, C. and Kieselbach, T. and Schroder, W. P. and Norling, B.},\n\tmonth = jun,\n\tyear = {2004},\n\tnote = {Place: Rockville\nPublisher: Amer Soc Biochemistry Molecular Biology Inc\nWOS:000222029100007},\n\tkeywords = {crystal-structure, escherichia-coli, genetic-analysis, htra family, multidrug efflux, photosystem-ii, plasma-membranes, protein, pseudomonas-aeruginosa, thylakoid membranes},\n\tpages = {586--595},\n}\n\n\n\n","author_short":["Huang, F.","Hedman, E.","Funk, C.","Kieselbach, T.","Schroder, W. 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