@article{
 title = {High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI.},
 type = {article},
 year = {2013},
 identifiers = {[object Object]},
 keywords = {Arabidopsis,Arabidopsis: genetics,Cytosine,Cytosine: metabolism,DNA Methylation,DNA Restriction Enzymes,DNA Restriction Enzymes: metabolism,High-Throughput Nucleotide Sequencing},
 pages = {56},
 volume = {14},
 websites = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3718668&tool=pmcentrez&rendertype=abstract},
 publisher = {BioMed Central},
 id = {7fcb10cd-82e2-329b-87fd-5dd2ee741805},
 created = {2017-10-14T10:53:33.669Z},
 accessed = {2016-07-01},
 file_attached = {false},
 profile_id = {57cbaa4c-3609-3597-b91c-bd12e56638fb},
 group_id = {b97159aa-8fdc-3227-aa16-9de80bf090dd},
 last_modified = {2017-10-14T10:53:33.669Z},
 read = {true},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {Huang2013a},
 private_publication = {false},
 abstract = {BACKGROUND: As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role in many biological processes. Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection.\n\nRESULTS: In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method.\n\nCONCLUSIONS: According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.},
 bibtype = {article},
 author = {Huang, Xiaojun and Lu, Hanlin and Wang, Jun-Wen and Xu, Liqin and Liu, Siyang and Sun, Jihua and Gao, Fei},
 journal = {BMC genetics},
 number = {1}
}

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Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection.\\n\\nRESULTS: In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method.\\n\\nCONCLUSIONS: According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.","bibtype":"article","author":"Huang, Xiaojun and Lu, Hanlin and Wang, Jun-Wen and Xu, Liqin and Liu, Siyang and Sun, Jihua and Gao, Fei","journal":"BMC genetics","number":"1","bibtex":"@article{\n title = {High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI.},\n type = {article},\n year = {2013},\n identifiers = {[object Object]},\n keywords = {Arabidopsis,Arabidopsis: genetics,Cytosine,Cytosine: metabolism,DNA Methylation,DNA Restriction Enzymes,DNA Restriction Enzymes: metabolism,High-Throughput Nucleotide Sequencing},\n pages = {56},\n volume = {14},\n websites = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3718668&tool=pmcentrez&rendertype=abstract},\n publisher = {BioMed Central},\n id = {7fcb10cd-82e2-329b-87fd-5dd2ee741805},\n created = {2017-10-14T10:53:33.669Z},\n accessed = {2016-07-01},\n file_attached = {false},\n profile_id = {57cbaa4c-3609-3597-b91c-bd12e56638fb},\n group_id = {b97159aa-8fdc-3227-aa16-9de80bf090dd},\n last_modified = {2017-10-14T10:53:33.669Z},\n read = {true},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n citation_key = {Huang2013a},\n private_publication = {false},\n abstract = {BACKGROUND: As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role in many biological processes. Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection.\\n\\nRESULTS: In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method.\\n\\nCONCLUSIONS: According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.},\n bibtype = {article},\n author = {Huang, Xiaojun and Lu, Hanlin and Wang, Jun-Wen and Xu, Liqin and Liu, Siyang and Sun, Jihua and Gao, Fei},\n journal = {BMC genetics},\n number = {1}\n}","author_short":["Huang, X.","Lu, H.","Wang, J.","Xu, L.","Liu, S.","Sun, J.","Gao, F."],"urls":{"Website":"http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3718668&tool=pmcentrez&rendertype=abstract"},"bibbaseid":"huang-lu-wang-xu-liu-sun-gao-highthroughputsequencingofmethylatedcytosineenrichedbymodificationdependentrestrictionendonucleasemspji-2013","role":"author","keyword":["Arabidopsis","Arabidopsis: genetics","Cytosine","Cytosine: metabolism","DNA Methylation","DNA Restriction Enzymes","DNA Restriction Enzymes: metabolism","High-Throughput Nucleotide Sequencing"],"downloads":0},"search_terms":["high","throughput","sequencing","methylated","cytosine","enriched","modification","dependent","restriction","endonuclease","mspji","huang","lu","wang","xu","liu","sun","gao"],"keywords":["arabidopsis","arabidopsis: genetics","cytosine","cytosine: metabolism","dna methylation","dna restriction enzymes","dna restriction enzymes: metabolism","high-throughput nucleotide sequencing"],"authorIDs":[]}