GFP-tagged pollen to monitor pollen flow of transgenic plants. Hudson, L., C., Chamberlain, D., & Stewart, C., N. Molecular Ecology Notes, 1(4):321-324, 2001.
abstract   bibtex   
In this study, the pollen-active LAT59 promoter from tomato was used to express a green fluorescent protein (GFP) encoding gene in Nicotiana tabacum (tobacco) pollen. This promoter is preferentially expressed in anthers and pollen. Pollen in transgenic plants segregated in a 1 : 1 Mendelian ratio, and the plants were polymerase chain reaction (PCR)-positive. GFP- tagged pollen was developed as a tool for tracking the movement of transgenic plant pollen in the environment. Specifically, it should be a useful tool for characterizing the spatial distribution patterns of transgenic pollen, to determine pollination mechanisms, to monitor the effects on nontarget organisms, and to monitor gene flow in field conditions.
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 title = {GFP-tagged pollen to monitor pollen flow of transgenic plants},
 type = {article},
 year = {2001},
 pages = {321-324},
 volume = {1},
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 abstract = {In this study, the pollen-active LAT59 promoter from tomato was used to express a green fluorescent protein (GFP) encoding gene in Nicotiana tabacum (tobacco) pollen. This promoter is preferentially expressed in anthers and pollen. Pollen in transgenic plants segregated in a 1 : 1 Mendelian ratio, and the plants were polymerase chain reaction (PCR)-positive. GFP- tagged pollen was developed as a tool for tracking the movement of transgenic plant pollen in the environment. Specifically, it should be a useful tool for characterizing the spatial distribution patterns of transgenic pollen, to determine pollination mechanisms, to monitor the effects on nontarget organisms, and to monitor gene flow in field conditions.},
 bibtype = {article},
 author = {Hudson, L C and Chamberlain, D and Stewart, C N},
 journal = {Molecular Ecology Notes},
 number = {4}
}
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