@article{RN160,
   author = {Hyler, A. R. and Baudoin, N. C. and Brown, M. S. and Stremler, M. A. and Cimini, D. and Davalos, R. V. and Schmelz, E. M.},
   title = {Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability},
   journal = {PLoS One},
   volume = {13},
   number = {3},
   pages = {e0194170},
   note = {1932-6203
Hyler, Alexandra R
Orcid: 0000-0002-8698-4455
Baudoin, Nicolaas C
Brown, Megan S
Stremler, Mark A
Cimini, Daniela
Davalos, Rafael V
Schmelz, Eva M
Journal Article
Research Support, Non-U.S. Gov't
United States
2018/03/23
PLoS One. 2018 Mar 22;13(3):e0194170. doi: 10.1371/journal.pone.0194170. eCollection 2018.},
   abstract = {Ovarian cancer cells are exposed to physical stress in the peritoneal cavity during both tumor growth and dissemination. Ascites build-up in metastatic ovarian cancer further increases the exposure to fluid shear stress. Here, we used a murine, in vitro ovarian cancer progression model in parallel with immortalized human cells to investigate how ovarian cancer cells of increasing aggressiveness respond to [Formula: see text] of fluid-induced shear stress. This biophysical stimulus significantly reduced cell viability in all cells exposed, independent of disease stage. Fluid shear stress induced spheroid formation and altered cytoskeleton organization in more tumorigenic cell lines. While benign ovarian cells appeared to survive in higher numbers under the influence of fluid shear stress, they exhibited severe morphological changes and chromosomal instability. These results suggest that exposure of benign cells to low magnitude fluid shear stress can induce phenotypic changes that are associated with transformation and ovarian cancer progression. Moreover, exposure of tumorigenic cells to fluid shear stress enhanced anchorage-independent survival, suggesting a role in promoting invasion and metastasis.},
   keywords = {Animals
Cell Line, Tumor
Cell Survival
Cytoskeleton/*metabolism/pathology
Female
*Genomic Instability
Humans
Mice
Neoplasm Invasiveness
Neoplasm Metastasis
Ovarian Neoplasms/*metabolism/pathology
*Shear Strength
*Stress, Mechanical},
   ISSN = {1932-6203},
   DOI = {10.1371/journal.pone.0194170},
   year = {2018},
   type = {Journal Article}
}

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M."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Hyler"],"firstnames":["A.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Baudoin"],"firstnames":["N.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Brown"],"firstnames":["M.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Stremler"],"firstnames":["M.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Cimini"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Schmelz"],"firstnames":["E.","M."],"suffixes":[]}],"title":"Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability","journal":"PLoS One","volume":"13","number":"3","pages":"e0194170","note":"1932-6203 Hyler, Alexandra R Orcid: 0000-0002-8698-4455 Baudoin, Nicolaas C Brown, Megan S Stremler, Mark A Cimini, Daniela Davalos, Rafael V Schmelz, Eva M Journal Article Research Support, Non-U.S. Gov't United States 2018/03/23 PLoS One. 2018 Mar 22;13(3):e0194170. doi: 10.1371/journal.pone.0194170. eCollection 2018.","abstract":"Ovarian cancer cells are exposed to physical stress in the peritoneal cavity during both tumor growth and dissemination. Ascites build-up in metastatic ovarian cancer further increases the exposure to fluid shear stress. Here, we used a murine, in vitro ovarian cancer progression model in parallel with immortalized human cells to investigate how ovarian cancer cells of increasing aggressiveness respond to [Formula: see text] of fluid-induced shear stress. This biophysical stimulus significantly reduced cell viability in all cells exposed, independent of disease stage. Fluid shear stress induced spheroid formation and altered cytoskeleton organization in more tumorigenic cell lines. While benign ovarian cells appeared to survive in higher numbers under the influence of fluid shear stress, they exhibited severe morphological changes and chromosomal instability. These results suggest that exposure of benign cells to low magnitude fluid shear stress can induce phenotypic changes that are associated with transformation and ovarian cancer progression. Moreover, exposure of tumorigenic cells to fluid shear stress enhanced anchorage-independent survival, suggesting a role in promoting invasion and metastasis.","keywords":"Animals Cell Line, Tumor Cell Survival Cytoskeleton/*metabolism/pathology Female *Genomic Instability Humans Mice Neoplasm Invasiveness Neoplasm Metastasis Ovarian Neoplasms/*metabolism/pathology *Shear Strength *Stress, Mechanical","issn":"1932-6203","doi":"10.1371/journal.pone.0194170","year":"2018","bibtex":"@article{RN160,\n author = {Hyler, A. R. and Baudoin, N. C. and Brown, M. S. and Stremler, M. A. and Cimini, D. and Davalos, R. V. and Schmelz, E. M.},\n title = {Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability},\n journal = {PLoS One},\n volume = {13},\n number = {3},\n pages = {e0194170},\n note = {1932-6203\nHyler, Alexandra R\nOrcid: 0000-0002-8698-4455\nBaudoin, Nicolaas C\nBrown, Megan S\nStremler, Mark A\nCimini, Daniela\nDavalos, Rafael V\nSchmelz, Eva M\nJournal Article\nResearch Support, Non-U.S. Gov't\nUnited States\n2018/03/23\nPLoS One. 2018 Mar 22;13(3):e0194170. doi: 10.1371/journal.pone.0194170. eCollection 2018.},\n abstract = {Ovarian cancer cells are exposed to physical stress in the peritoneal cavity during both tumor growth and dissemination. Ascites build-up in metastatic ovarian cancer further increases the exposure to fluid shear stress. Here, we used a murine, in vitro ovarian cancer progression model in parallel with immortalized human cells to investigate how ovarian cancer cells of increasing aggressiveness respond to [Formula: see text] of fluid-induced shear stress. This biophysical stimulus significantly reduced cell viability in all cells exposed, independent of disease stage. Fluid shear stress induced spheroid formation and altered cytoskeleton organization in more tumorigenic cell lines. While benign ovarian cells appeared to survive in higher numbers under the influence of fluid shear stress, they exhibited severe morphological changes and chromosomal instability. These results suggest that exposure of benign cells to low magnitude fluid shear stress can induce phenotypic changes that are associated with transformation and ovarian cancer progression. Moreover, exposure of tumorigenic cells to fluid shear stress enhanced anchorage-independent survival, suggesting a role in promoting invasion and metastasis.},\n keywords = {Animals\nCell Line, Tumor\nCell Survival\nCytoskeleton/*metabolism/pathology\nFemale\n*Genomic Instability\nHumans\nMice\nNeoplasm Invasiveness\nNeoplasm Metastasis\nOvarian Neoplasms/*metabolism/pathology\n*Shear Strength\n*Stress, Mechanical},\n ISSN = {1932-6203},\n DOI = {10.1371/journal.pone.0194170},\n year = {2018},\n type = {Journal Article}\n}\n\n","author_short":["Hyler, A. R.","Baudoin, N. C.","Brown, M. S.","Stremler, M. A.","Cimini, D.","Davalos, R. V.","Schmelz, E. M."],"key":"RN160","id":"RN160","bibbaseid":"hyler-baudoin-brown-stremler-cimini-davalos-schmelz-fluidshearstressimpactsovariancancercellviabilitysubcellularorganizationandpromotesgenomicinstability-2018","role":"author","urls":{},"keyword":["Animals Cell Line","Tumor Cell Survival Cytoskeleton/*metabolism/pathology Female *Genomic Instability Humans Mice Neoplasm Invasiveness Neoplasm Metastasis Ovarian Neoplasms/*metabolism/pathology *Shear Strength *Stress","Mechanical"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["animals cell line","tumor cell survival cytoskeleton/*metabolism/pathology female *genomic instability humans mice neoplasm invasiveness neoplasm metastasis ovarian neoplasms/*metabolism/pathology *shear strength *stress","mechanical"],"search_terms":["fluid","shear","stress","impacts","ovarian","cancer","cell","viability","subcellular","organization","promotes","genomic","instability","hyler","baudoin","brown","stremler","cimini","davalos","schmelz"],"title":"Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability","year":2018}