@article{RN120,
   author = {Hyler, A. R. and Hong, D. and Davalos, R. V. and Swami, N. S. and Schmelz, E. M.},
   title = {A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency},
   journal = {Electrophoresis},
   volume = {42},
   number = {12-13},
   pages = {1366-1377},
   note = {1522-2683
Hyler, Alexandra R
Orcid: 0000-0002-8698-4455
Hong, Daly
Davalos, Rafael V
Swami, Nathan S
Schmelz, Eva M
R21 CA173092/CA/NCI NIH HHS/United States
Journal Article
Research Support, Non-U.S. Gov't
Germany
2021/03/10
Electrophoresis. 2021 Jul;42(12-13):1366-1377. doi: 10.1002/elps.202000324. Epub 2021 Apr 15.},
   abstract = {Cell separation has become a critical diagnostic, research, and treatment tool for personalized medicine. Despite significant advances in cell separation, most widely used applications require the use of multiple, expensive antibodies to known markers in order to identify subpopulations of cells for separation. Dielectrophoresis (DEP) provides a biophysical separation technique that can target cell subpopulations based on phenotype without labels and return native cells for downstream analysis. One challenge in employing any DEP device is the sample being separated must be transferred into an ultralow conductivity medium, which can be detrimental in retaining cells' native phenotypes for separation. Here, we measured properties of traditional DEP reagents and determined that after just 1-2 h of exposure and subsequent culture, cells' viability was significantly reduced below 50%. We developed and tested a novel buffer (Cyto Buffer) that achieved 6 weeks of stable shelf-life and demonstrated significantly improved viability and physiological properties. We then determined the impact of Cyto Buffer on cells' dielectric properties and morphology and found that cells retained properties more similar to that of their native media. Finally, we vetted Cyto Buffer's usability on a cell separation platform (Cyto R1) to determine combined efficacy for cell separations. Here, more than 80% of cells from different cell lines were recovered and were determined to be >70% viable following exposure to Cyto Buffer, flow stimulation, electromanipulation, and downstream collection and growth. The developed buffer demonstrated improved opportunities for electrical cell manipulation, enrichment, and recovery for next generation cell separations.},
   keywords = {Cell Line
Cell Separation
Cell Survival
Culture Media
*Electric Conductivity
Electrophoresis
Buffer conductivity
Dielectrophoresis
Electromanipulation
Microfluidics},
   ISSN = {0173-0835},
   DOI = {10.1002/elps.202000324},
   year = {2021},
   type = {Journal Article}
}

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M."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Hyler"],"firstnames":["A.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Hong"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Swami"],"firstnames":["N.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Schmelz"],"firstnames":["E.","M."],"suffixes":[]}],"title":"A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency","journal":"Electrophoresis","volume":"42","number":"12-13","pages":"1366-1377","note":"1522-2683 Hyler, Alexandra R Orcid: 0000-0002-8698-4455 Hong, Daly Davalos, Rafael V Swami, Nathan S Schmelz, Eva M R21 CA173092/CA/NCI NIH HHS/United States Journal Article Research Support, Non-U.S. Gov't Germany 2021/03/10 Electrophoresis. 2021 Jul;42(12-13):1366-1377. doi: 10.1002/elps.202000324. Epub 2021 Apr 15.","abstract":"Cell separation has become a critical diagnostic, research, and treatment tool for personalized medicine. Despite significant advances in cell separation, most widely used applications require the use of multiple, expensive antibodies to known markers in order to identify subpopulations of cells for separation. Dielectrophoresis (DEP) provides a biophysical separation technique that can target cell subpopulations based on phenotype without labels and return native cells for downstream analysis. One challenge in employing any DEP device is the sample being separated must be transferred into an ultralow conductivity medium, which can be detrimental in retaining cells' native phenotypes for separation. Here, we measured properties of traditional DEP reagents and determined that after just 1-2 h of exposure and subsequent culture, cells' viability was significantly reduced below 50%. We developed and tested a novel buffer (Cyto Buffer) that achieved 6 weeks of stable shelf-life and demonstrated significantly improved viability and physiological properties. We then determined the impact of Cyto Buffer on cells' dielectric properties and morphology and found that cells retained properties more similar to that of their native media. Finally, we vetted Cyto Buffer's usability on a cell separation platform (Cyto R1) to determine combined efficacy for cell separations. Here, more than 80% of cells from different cell lines were recovered and were determined to be >70% viable following exposure to Cyto Buffer, flow stimulation, electromanipulation, and downstream collection and growth. The developed buffer demonstrated improved opportunities for electrical cell manipulation, enrichment, and recovery for next generation cell separations.","keywords":"Cell Line Cell Separation Cell Survival Culture Media *Electric Conductivity Electrophoresis Buffer conductivity Dielectrophoresis Electromanipulation Microfluidics","issn":"0173-0835","doi":"10.1002/elps.202000324","year":"2021","bibtex":"@article{RN120,\n author = {Hyler, A. R. and Hong, D. and Davalos, R. V. and Swami, N. S. and Schmelz, E. M.},\n title = {A novel ultralow conductivity electromanipulation buffer improves cell viability and enhances dielectrophoretic consistency},\n journal = {Electrophoresis},\n volume = {42},\n number = {12-13},\n pages = {1366-1377},\n note = {1522-2683\nHyler, Alexandra R\nOrcid: 0000-0002-8698-4455\nHong, Daly\nDavalos, Rafael V\nSwami, Nathan S\nSchmelz, Eva M\nR21 CA173092/CA/NCI NIH HHS/United States\nJournal Article\nResearch Support, Non-U.S. Gov't\nGermany\n2021/03/10\nElectrophoresis. 2021 Jul;42(12-13):1366-1377. doi: 10.1002/elps.202000324. Epub 2021 Apr 15.},\n abstract = {Cell separation has become a critical diagnostic, research, and treatment tool for personalized medicine. Despite significant advances in cell separation, most widely used applications require the use of multiple, expensive antibodies to known markers in order to identify subpopulations of cells for separation. Dielectrophoresis (DEP) provides a biophysical separation technique that can target cell subpopulations based on phenotype without labels and return native cells for downstream analysis. One challenge in employing any DEP device is the sample being separated must be transferred into an ultralow conductivity medium, which can be detrimental in retaining cells' native phenotypes for separation. Here, we measured properties of traditional DEP reagents and determined that after just 1-2 h of exposure and subsequent culture, cells' viability was significantly reduced below 50%. We developed and tested a novel buffer (Cyto Buffer) that achieved 6 weeks of stable shelf-life and demonstrated significantly improved viability and physiological properties. We then determined the impact of Cyto Buffer on cells' dielectric properties and morphology and found that cells retained properties more similar to that of their native media. Finally, we vetted Cyto Buffer's usability on a cell separation platform (Cyto R1) to determine combined efficacy for cell separations. Here, more than 80% of cells from different cell lines were recovered and were determined to be >70% viable following exposure to Cyto Buffer, flow stimulation, electromanipulation, and downstream collection and growth. The developed buffer demonstrated improved opportunities for electrical cell manipulation, enrichment, and recovery for next generation cell separations.},\n keywords = {Cell Line\nCell Separation\nCell Survival\nCulture Media\n*Electric Conductivity\nElectrophoresis\nBuffer conductivity\nDielectrophoresis\nElectromanipulation\nMicrofluidics},\n ISSN = {0173-0835},\n DOI = {10.1002/elps.202000324},\n year = {2021},\n type = {Journal Article}\n}\n\n","author_short":["Hyler, A. R.","Hong, D.","Davalos, R. V.","Swami, N. S.","Schmelz, E. 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