Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biphenyl mixture, BP-6.

Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biphenyl mixture, BP-6. Ibhazehiebo, K., Iwasaki, T., Okano-Uchida, T., Shimokawa, N., Ishizaki, Y., & Koibuchi, N. Neurotoxicology, 32(4):400–9, August, 2011.

Paper doi abstract bibtex

Polybrominated biphenyls (PBBs) are polyhalogenated, bioaccumulative flame retardant chemicals, which have been used in a variety of consumer and household products. They were accidentally introduced into the food chain in Michigan in 1973 and have remained a source of health concern. Studies have shown that exposure to PBB may cause adverse neurotoxic effects. We therefore examined the effects of BP-6, a PBB mixture, on thyroid hormone (TH) receptor (TR)-mediated transcription, on TH-induced Purkinje cell dendritogenesis, and on TH-induced cerebellar granule cell neurite extension. Our study shows that BP-6 suppressed TR-mediated transcription in CV-1 cells. Mammalian two-hybrid studies revealed that BP-6 did not inhibit coactivator binding to TR nor did it recruit corepressors to TR. Further examination using the liquid chemiluminescent DNA pull down assay revealed partial dissociation of TR from TH response element (TRE). In primary rat cerebellar culture, BP-6 significantly suppressed TH-induced dendrite arborization of Purkinje cells, and in reaggregate rat granule cell culture, impaired TH-induced neurite extension of granule cells. Taken together, our results indicate that BP-6 may disrupt TH homeostasis and consequently impair normal neuronal development.

@article{ibhazehiebo_suppression_2011,
	title = {Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biphenyl mixture, {BP}-6.},
	volume = {32},
	issn = {1872-9711},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/21396401},
	doi = {10.1016/j.neuro.2011.02.008},
	abstract = {Polybrominated biphenyls (PBBs) are polyhalogenated, bioaccumulative flame retardant chemicals, which have been used in a variety of consumer and household products. They were accidentally introduced into the food chain in Michigan in 1973 and have remained a source of health concern. Studies have shown that exposure to PBB may cause adverse neurotoxic effects. We therefore examined the effects of BP-6, a PBB mixture, on thyroid hormone (TH) receptor (TR)-mediated transcription, on TH-induced Purkinje cell dendritogenesis, and on TH-induced cerebellar granule cell neurite extension. Our study shows that BP-6 suppressed TR-mediated transcription in CV-1 cells. Mammalian two-hybrid studies revealed that BP-6 did not inhibit coactivator binding to TR nor did it recruit corepressors to TR. Further examination using the liquid chemiluminescent DNA pull down assay revealed partial dissociation of TR from TH response element (TRE). In primary rat cerebellar culture, BP-6 significantly suppressed TH-induced dendrite arborization of Purkinje cells, and in reaggregate rat granule cell culture, impaired TH-induced neurite extension of granule cells. Taken together, our results indicate that BP-6 may disrupt TH homeostasis and consequently impair normal neuronal development.},
	number = {4},
	journal = {Neurotoxicology},
	author = {Ibhazehiebo, Kingsley and Iwasaki, Toshiharu and Okano-Uchida, Takayuki and Shimokawa, Noriaki and Ishizaki, Yasuki and Koibuchi, Noriyuki},
	month = aug,
	year = {2011},
	pmid = {21396401},
	keywords = {Animals, Binding Sites, Cercopithecus aethiops, Cerebellum, Cerebellum: drug effects, Cerebellum: growth \& development, Cerebellum: metabolism, Cerebellum: pathology, Dendrites, Dendrites: drug effects, Dendrites: metabolism, Dendrites: pathology, Dose-Response Relationship, Down-Regulation, Drug, Flame Retardants: toxicity, Flame retardants, Genetic, Genetic: drug effects, Glucocorticoid, Glucocorticoid: drug effects, Glucocorticoid: metabolism, Neurites, Neurites: drug effects, Neurites: metabolism, Neurites: pathology, Neurogenesis, Neurogenesis: drug effects, Newborn, Polybrominated Biphenyls, Polybrominated Biphenyls: toxicity, Purkinje Cells, Purkinje Cells: drug effects, Purkinje Cells: metabolism, Purkinje Cells: pathology, Rats, Receptors, Response Elements, Response Elements: drug effects, Thyroid Hormone Receptors beta, Thyroid Hormone Receptors beta: drug effects, Thyroid Hormone Receptors beta: genetics, Thyroid Hormone Receptors beta: metabolism, Thyroxine, Thyroxine: metabolism, Transcription, Transfection, Triiodothyronine, Triiodothyronine: metabolism, Wistar, cell line},
	pages = {400--9},
}

Downloads: 0

{"_id":"PJDhz3qvSZtAwZG7F","bibbaseid":"ibhazehiebo-iwasaki-okanouchida-shimokawa-ishizaki-koibuchi-suppressionofthyroidhormonereceptormediatedtranscriptionanddisruptionofthyroidhormoneinducedcerebellarmorphogenesisbythepolybrominatedbiphenylmixturebp6-2011","author_short":["Ibhazehiebo, K.","Iwasaki, T.","Okano-Uchida, T.","Shimokawa, N.","Ishizaki, Y.","Koibuchi, N."],"bibdata":{"bibtype":"article","type":"article","title":"Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biphenyl mixture, BP-6.","volume":"32","issn":"1872-9711","url":"http://www.ncbi.nlm.nih.gov/pubmed/21396401","doi":"10.1016/j.neuro.2011.02.008","abstract":"Polybrominated biphenyls (PBBs) are polyhalogenated, bioaccumulative flame retardant chemicals, which have been used in a variety of consumer and household products. They were accidentally introduced into the food chain in Michigan in 1973 and have remained a source of health concern. Studies have shown that exposure to PBB may cause adverse neurotoxic effects. We therefore examined the effects of BP-6, a PBB mixture, on thyroid hormone (TH) receptor (TR)-mediated transcription, on TH-induced Purkinje cell dendritogenesis, and on TH-induced cerebellar granule cell neurite extension. Our study shows that BP-6 suppressed TR-mediated transcription in CV-1 cells. Mammalian two-hybrid studies revealed that BP-6 did not inhibit coactivator binding to TR nor did it recruit corepressors to TR. Further examination using the liquid chemiluminescent DNA pull down assay revealed partial dissociation of TR from TH response element (TRE). In primary rat cerebellar culture, BP-6 significantly suppressed TH-induced dendrite arborization of Purkinje cells, and in reaggregate rat granule cell culture, impaired TH-induced neurite extension of granule cells. Taken together, our results indicate that BP-6 may disrupt TH homeostasis and consequently impair normal neuronal development.","number":"4","journal":"Neurotoxicology","author":[{"propositions":[],"lastnames":["Ibhazehiebo"],"firstnames":["Kingsley"],"suffixes":[]},{"propositions":[],"lastnames":["Iwasaki"],"firstnames":["Toshiharu"],"suffixes":[]},{"propositions":[],"lastnames":["Okano-Uchida"],"firstnames":["Takayuki"],"suffixes":[]},{"propositions":[],"lastnames":["Shimokawa"],"firstnames":["Noriaki"],"suffixes":[]},{"propositions":[],"lastnames":["Ishizaki"],"firstnames":["Yasuki"],"suffixes":[]},{"propositions":[],"lastnames":["Koibuchi"],"firstnames":["Noriyuki"],"suffixes":[]}],"month":"August","year":"2011","pmid":"21396401","keywords":"Animals, Binding Sites, Cercopithecus aethiops, Cerebellum, Cerebellum: drug effects, Cerebellum: growth & development, Cerebellum: metabolism, Cerebellum: pathology, Dendrites, Dendrites: drug effects, Dendrites: metabolism, Dendrites: pathology, Dose-Response Relationship, Down-Regulation, Drug, Flame Retardants: toxicity, Flame retardants, Genetic, Genetic: drug effects, Glucocorticoid, Glucocorticoid: drug effects, Glucocorticoid: metabolism, Neurites, Neurites: drug effects, Neurites: metabolism, Neurites: pathology, Neurogenesis, Neurogenesis: drug effects, Newborn, Polybrominated Biphenyls, Polybrominated Biphenyls: toxicity, Purkinje Cells, Purkinje Cells: drug effects, Purkinje Cells: metabolism, Purkinje Cells: pathology, Rats, Receptors, Response Elements, Response Elements: drug effects, Thyroid Hormone Receptors beta, Thyroid Hormone Receptors beta: drug effects, Thyroid Hormone Receptors beta: genetics, Thyroid Hormone Receptors beta: metabolism, Thyroxine, Thyroxine: metabolism, Transcription, Transfection, Triiodothyronine, Triiodothyronine: metabolism, Wistar, cell line","pages":"400–9","bibtex":"@article{ibhazehiebo_suppression_2011,\n\ttitle = {Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biphenyl mixture, {BP}-6.},\n\tvolume = {32},\n\tissn = {1872-9711},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/21396401},\n\tdoi = {10.1016/j.neuro.2011.02.008},\n\tabstract = {Polybrominated biphenyls (PBBs) are polyhalogenated, bioaccumulative flame retardant chemicals, which have been used in a variety of consumer and household products. They were accidentally introduced into the food chain in Michigan in 1973 and have remained a source of health concern. Studies have shown that exposure to PBB may cause adverse neurotoxic effects. We therefore examined the effects of BP-6, a PBB mixture, on thyroid hormone (TH) receptor (TR)-mediated transcription, on TH-induced Purkinje cell dendritogenesis, and on TH-induced cerebellar granule cell neurite extension. Our study shows that BP-6 suppressed TR-mediated transcription in CV-1 cells. Mammalian two-hybrid studies revealed that BP-6 did not inhibit coactivator binding to TR nor did it recruit corepressors to TR. Further examination using the liquid chemiluminescent DNA pull down assay revealed partial dissociation of TR from TH response element (TRE). In primary rat cerebellar culture, BP-6 significantly suppressed TH-induced dendrite arborization of Purkinje cells, and in reaggregate rat granule cell culture, impaired TH-induced neurite extension of granule cells. Taken together, our results indicate that BP-6 may disrupt TH homeostasis and consequently impair normal neuronal development.},\n\tnumber = {4},\n\tjournal = {Neurotoxicology},\n\tauthor = {Ibhazehiebo, Kingsley and Iwasaki, Toshiharu and Okano-Uchida, Takayuki and Shimokawa, Noriaki and Ishizaki, Yasuki and Koibuchi, Noriyuki},\n\tmonth = aug,\n\tyear = {2011},\n\tpmid = {21396401},\n\tkeywords = {Animals, Binding Sites, Cercopithecus aethiops, Cerebellum, Cerebellum: drug effects, Cerebellum: growth \\& development, Cerebellum: metabolism, Cerebellum: pathology, Dendrites, Dendrites: drug effects, Dendrites: metabolism, Dendrites: pathology, Dose-Response Relationship, Down-Regulation, Drug, Flame Retardants: toxicity, Flame retardants, Genetic, Genetic: drug effects, Glucocorticoid, Glucocorticoid: drug effects, Glucocorticoid: metabolism, Neurites, Neurites: drug effects, Neurites: metabolism, Neurites: pathology, Neurogenesis, Neurogenesis: drug effects, Newborn, Polybrominated Biphenyls, Polybrominated Biphenyls: toxicity, Purkinje Cells, Purkinje Cells: drug effects, Purkinje Cells: metabolism, Purkinje Cells: pathology, Rats, Receptors, Response Elements, Response Elements: drug effects, Thyroid Hormone Receptors beta, Thyroid Hormone Receptors beta: drug effects, Thyroid Hormone Receptors beta: genetics, Thyroid Hormone Receptors beta: metabolism, Thyroxine, Thyroxine: metabolism, Transcription, Transfection, Triiodothyronine, Triiodothyronine: metabolism, Wistar, cell line},\n\tpages = {400--9},\n}\n\n","author_short":["Ibhazehiebo, K.","Iwasaki, T.","Okano-Uchida, T.","Shimokawa, N.","Ishizaki, Y.","Koibuchi, N."],"key":"ibhazehiebo_suppression_2011","id":"ibhazehiebo_suppression_2011","bibbaseid":"ibhazehiebo-iwasaki-okanouchida-shimokawa-ishizaki-koibuchi-suppressionofthyroidhormonereceptormediatedtranscriptionanddisruptionofthyroidhormoneinducedcerebellarmorphogenesisbythepolybrominatedbiphenylmixturebp6-2011","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/21396401"},"keyword":["Animals","Binding Sites","Cercopithecus aethiops","Cerebellum","Cerebellum: drug effects","Cerebellum: growth & development","Cerebellum: metabolism","Cerebellum: pathology","Dendrites","Dendrites: drug effects","Dendrites: metabolism","Dendrites: pathology","Dose-Response Relationship","Down-Regulation","Drug","Flame Retardants: toxicity","Flame retardants","Genetic","Genetic: drug effects","Glucocorticoid","Glucocorticoid: drug effects","Glucocorticoid: metabolism","Neurites","Neurites: drug effects","Neurites: metabolism","Neurites: pathology","Neurogenesis","Neurogenesis: drug effects","Newborn","Polybrominated Biphenyls","Polybrominated Biphenyls: toxicity","Purkinje Cells","Purkinje Cells: drug effects","Purkinje Cells: metabolism","Purkinje Cells: pathology","Rats","Receptors","Response Elements","Response Elements: drug effects","Thyroid Hormone Receptors beta","Thyroid Hormone Receptors beta: drug effects","Thyroid Hormone Receptors beta: genetics","Thyroid Hormone Receptors beta: metabolism","Thyroxine","Thyroxine: metabolism","Transcription","Transfection","Triiodothyronine","Triiodothyronine: metabolism","Wistar","cell line"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"http://bibbase.org/zotero/mdedeo","dataSources":["SSfxPwd7XbkRtnZ9f"],"keywords":["animals","binding sites","cercopithecus aethiops","cerebellum","cerebellum: drug effects","cerebellum: growth & development","cerebellum: metabolism","cerebellum: pathology","dendrites","dendrites: drug effects","dendrites: metabolism","dendrites: pathology","dose-response relationship","down-regulation","drug","flame retardants: toxicity","flame retardants","genetic","genetic: drug effects","glucocorticoid","glucocorticoid: drug effects","glucocorticoid: metabolism","neurites","neurites: drug effects","neurites: metabolism","neurites: pathology","neurogenesis","neurogenesis: drug effects","newborn","polybrominated biphenyls","polybrominated biphenyls: toxicity","purkinje cells","purkinje cells: drug effects","purkinje cells: metabolism","purkinje cells: pathology","rats","receptors","response elements","response elements: drug effects","thyroid hormone receptors beta","thyroid hormone receptors beta: drug effects","thyroid hormone receptors beta: genetics","thyroid hormone receptors beta: metabolism","thyroxine","thyroxine: metabolism","transcription","transfection","triiodothyronine","triiodothyronine: metabolism","wistar","cell line"],"search_terms":["suppression","thyroid","hormone","receptor","mediated","transcription","disruption","thyroid","hormone","induced","cerebellar","morphogenesis","polybrominated","biphenyl","mixture","ibhazehiebo","iwasaki","okano-uchida","shimokawa","ishizaki","koibuchi"],"title":"Suppression of thyroid hormone receptor-mediated transcription and disruption of thyroid hormone-induced cerebellar morphogenesis by the polybrominated biphenyl mixture, BP-6.","year":2011}