Effects of Combination of Ketamine with NorBNI on DREAM Protein and Ketamine-Induced Antinociceptive Effect in Formalin-Induced Inflammatory Pain. Idris, L., Suppian, R., & Ismail, Z.
Effects of Combination of Ketamine with NorBNI on DREAM Protein and Ketamine-Induced Antinociceptive Effect in Formalin-Induced Inflammatory Pain [link]Website  abstract   bibtex   
Downstream Regulatory Element Antagonist Modulator (DREAM) protein modulates pain by regulating c-Fos and prodynorphin genes transcription. The present study investigates the changes of DREAM protein expression in rat’s spinal cord and ketamine-induced antinociceptive effects upon the combining administration of ketamine and nor-binaltorphimine dihydrochloride (norBNI) following formalininduced pain. Male Sprague Dawley rats were divided into several groups: rats administered with normal saline (C), rats given only formalin injections (F), rats treated with preemptive administration of either norBNI (N+F) or ketamine (K+F) with formalin injections, and the combination of norBNI and ketamine (NK+F) administration with formalin injection. Formalin (5%) was injected subcutaneously to the rats’ hind paws, and the pain behavior was recorded for one hour. After two hours, the rats were sacrificed, and their spinal cords (L4-L5) were removed for western blot analysis. Results: Ketamine-induced antinociceptive effect on the pain behavior responses were apparently suppressed following the combination with norBNI (NK+F). However, there was no change in spinal DREAM protein levels detected between K+F and NK+F group. Conclusion: The suppression effect of the ketamine-induced antinociceptive effect was attenuated when combined with norBNI, though not through the modulation of genes transcriptional mechanism regulated by the DREAM protein.
@article{
 title = {Effects of Combination of Ketamine with NorBNI on DREAM Protein and Ketamine-Induced Antinociceptive Effect in Formalin-Induced Inflammatory Pain},
 type = {article},
 websites = {https://www.researchgate.net/profile/Idris_Long/publication/322398561_Effects_of_combination_of_ketamine_with_NorBNI_on_DREAM_protein_and_ketamine_induced_antinociceptive_effect_in_formalin_induced_pain/links/5a577676a6fdcc30f86f2720/Effects-of-combinatio},
 id = {5b366913-80ea-311b-b79c-46c02789a007},
 created = {2018-02-01T09:09:46.953Z},
 accessed = {2018-01-31},
 file_attached = {false},
 profile_id = {078d13e5-632a-3ebf-9e0e-51a0b2c2c66e},
 group_id = {d9389c6c-8ab5-3b8b-86ed-33db09ca0198},
 last_modified = {2018-02-24T20:09:02.584Z},
 tags = {OA},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {Idris},
 notes = {LB},
 private_publication = {false},
 abstract = {Downstream Regulatory Element Antagonist Modulator (DREAM) protein modulates pain by
regulating c-Fos and prodynorphin genes transcription. The present study investigates the changes of
DREAM protein expression in rat’s spinal cord and ketamine-induced antinociceptive effects upon the
combining administration of ketamine and nor-binaltorphimine dihydrochloride (norBNI) following formalininduced
pain. Male Sprague Dawley rats were divided into several groups: rats administered with normal
saline (C), rats given only formalin injections (F), rats treated with preemptive administration of either norBNI
(N+F) or ketamine (K+F) with formalin injections, and the combination of norBNI and ketamine (NK+F)
administration with formalin injection. Formalin (5%) was injected subcutaneously to the rats’ hind paws, and
the pain behavior was recorded for one hour. After two hours, the rats were sacrificed, and their spinal cords
(L4-L5) were removed for western blot analysis. Results: Ketamine-induced antinociceptive effect on the pain
behavior responses were apparently suppressed following the combination with norBNI (NK+F). However,
there was no change in spinal DREAM protein levels detected between K+F and NK+F group. Conclusion:
The suppression effect of the ketamine-induced antinociceptive effect was attenuated when combined with
norBNI, though not through the modulation of genes transcriptional mechanism regulated by the DREAM
protein. 
},
 bibtype = {article},
 author = {Idris, Long and Suppian, Rapeah and Ismail, Zalina}
}
Downloads: 0