@article{igamberdiev_capacity_2000,
	title = {Capacity for {NADPH}/{NADP} turnover in the cytosol of barley seed endosperm: {The} role of {NADPH}-dependent hydroxypyruvate reductase},
	volume = {38},
	issn = {0981-9428},
	shorttitle = {Capacity for {NADPH}/{NADP} turnover in the cytosol of barley seed endosperm},
	url = {https://www.sciencedirect.com/science/article/pii/S0981942800011876},
	doi = {10/dps4mx},
	abstract = {Barley (Hordeum vulgare L.) endosperm from developing seeds was found to contain relatively high activities of cytosolic NAD(P)H-dependent hydroxypyruvate reductase (HPR-2) and isocitrate dehydrogenase (ICDH). In contrast, activities of peroxisomal NADH-dependent hydroxypyruvate reductase (HPR-1) and glycolate oxidase as well as cytosolic NAD(P)H-dependent glyoxylate reductase were very low or absent in the endosperm both during maturation and seed germination, indicating the lack of a complete glycolate cycle in this tissue. In addition, activities of cytosolic glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase were low or absent in the endosperm. The endosperm HPR-2 exhibited similar properties to those of an earlier described HPR-2 from green leaves, e.g. activities with both hydroxypyruvate and glyoxylate, utilization of both NADPH and NADH as cofactors, and a strong uncompetitive inhibition by oxalate (Ki in the order of micromolar). In etiolated leaves, both HPR-1 and HPR-2 were present with the same activity as in green leaves, indicating that the lack of HPR-1 in the endosperm is not a general feature of non-photosynthetic tissues. We conclude that the endosperm has considerable capacity for cytosolic NADP/NADPH cycling via HPR-2 and ICDH, the former being possibly involved in the utilization of a serine-derived carbon.},
	language = {en},
	number = {10},
	urldate = {2021-11-08},
	journal = {Plant Physiology and Biochemistry},
	author = {Igamberdiev, Abir U. and Kleczkowski, Leszek A.},
	month = oct,
	year = {2000},
	keywords = {cytosol, glyoxylate, hydroxypyruvate reductase, seed germination, starch synthesis, sucrose synthesis},
	pages = {747--753},
}

{"_id":"T5tJC5Pp5sbkdhH8a","bibbaseid":"igamberdiev-kleczkowski-capacityfornadphnadpturnoverinthecytosolofbarleyseedendospermtheroleofnadphdependenthydroxypyruvatereductase-2000","author_short":["Igamberdiev, A. U.","Kleczkowski, L. A."],"bibdata":{"bibtype":"article","type":"article","title":"Capacity for NADPH/NADP turnover in the cytosol of barley seed endosperm: The role of NADPH-dependent hydroxypyruvate reductase","volume":"38","issn":"0981-9428","shorttitle":"Capacity for NADPH/NADP turnover in the cytosol of barley seed endosperm","url":"https://www.sciencedirect.com/science/article/pii/S0981942800011876","doi":"10/dps4mx","abstract":"Barley (Hordeum vulgare L.) endosperm from developing seeds was found to contain relatively high activities of cytosolic NAD(P)H-dependent hydroxypyruvate reductase (HPR-2) and isocitrate dehydrogenase (ICDH). In contrast, activities of peroxisomal NADH-dependent hydroxypyruvate reductase (HPR-1) and glycolate oxidase as well as cytosolic NAD(P)H-dependent glyoxylate reductase were very low or absent in the endosperm both during maturation and seed germination, indicating the lack of a complete glycolate cycle in this tissue. In addition, activities of cytosolic glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase were low or absent in the endosperm. The endosperm HPR-2 exhibited similar properties to those of an earlier described HPR-2 from green leaves, e.g. activities with both hydroxypyruvate and glyoxylate, utilization of both NADPH and NADH as cofactors, and a strong uncompetitive inhibition by oxalate (Ki in the order of micromolar). In etiolated leaves, both HPR-1 and HPR-2 were present with the same activity as in green leaves, indicating that the lack of HPR-1 in the endosperm is not a general feature of non-photosynthetic tissues. We conclude that the endosperm has considerable capacity for cytosolic NADP/NADPH cycling via HPR-2 and ICDH, the former being possibly involved in the utilization of a serine-derived carbon.","language":"en","number":"10","urldate":"2021-11-08","journal":"Plant Physiology and Biochemistry","author":[{"propositions":[],"lastnames":["Igamberdiev"],"firstnames":["Abir","U."],"suffixes":[]},{"propositions":[],"lastnames":["Kleczkowski"],"firstnames":["Leszek","A."],"suffixes":[]}],"month":"October","year":"2000","keywords":"cytosol, glyoxylate, hydroxypyruvate reductase, seed germination, starch synthesis, sucrose synthesis","pages":"747–753","bibtex":"@article{igamberdiev_capacity_2000,\n\ttitle = {Capacity for {NADPH}/{NADP} turnover in the cytosol of barley seed endosperm: {The} role of {NADPH}-dependent hydroxypyruvate reductase},\n\tvolume = {38},\n\tissn = {0981-9428},\n\tshorttitle = {Capacity for {NADPH}/{NADP} turnover in the cytosol of barley seed endosperm},\n\turl = {https://www.sciencedirect.com/science/article/pii/S0981942800011876},\n\tdoi = {10/dps4mx},\n\tabstract = {Barley (Hordeum vulgare L.) endosperm from developing seeds was found to contain relatively high activities of cytosolic NAD(P)H-dependent hydroxypyruvate reductase (HPR-2) and isocitrate dehydrogenase (ICDH). In contrast, activities of peroxisomal NADH-dependent hydroxypyruvate reductase (HPR-1) and glycolate oxidase as well as cytosolic NAD(P)H-dependent glyoxylate reductase were very low or absent in the endosperm both during maturation and seed germination, indicating the lack of a complete glycolate cycle in this tissue. In addition, activities of cytosolic glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase were low or absent in the endosperm. The endosperm HPR-2 exhibited similar properties to those of an earlier described HPR-2 from green leaves, e.g. activities with both hydroxypyruvate and glyoxylate, utilization of both NADPH and NADH as cofactors, and a strong uncompetitive inhibition by oxalate (Ki in the order of micromolar). In etiolated leaves, both HPR-1 and HPR-2 were present with the same activity as in green leaves, indicating that the lack of HPR-1 in the endosperm is not a general feature of non-photosynthetic tissues. 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A."],"key":"igamberdiev_capacity_2000","id":"igamberdiev_capacity_2000","bibbaseid":"igamberdiev-kleczkowski-capacityfornadphnadpturnoverinthecytosolofbarleyseedendospermtheroleofnadphdependenthydroxypyruvatereductase-2000","role":"author","urls":{"Paper":"https://www.sciencedirect.com/science/article/pii/S0981942800011876"},"keyword":["cytosol","glyoxylate","hydroxypyruvate reductase","seed germination","starch synthesis","sucrose synthesis"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["nNTiC6NxTbGDoRMPx","3bgRArTPQuMzE5oHo","Tu3jPdZyJF3j547xT","9cGcv2t8pRzC92kzs","3zTPPmKj8BiTcpc6C"],"keywords":["cytosol","glyoxylate","hydroxypyruvate reductase","seed germination","starch synthesis","sucrose synthesis"],"search_terms":["capacity","nadph","nadp","turnover","cytosol","barley","seed","endosperm","role","nadph","dependent","hydroxypyruvate","reductase","igamberdiev","kleczkowski"],"title":"Capacity for NADPH/NADP turnover in the cytosol of barley seed endosperm: The role of NADPH-dependent hydroxypyruvate reductase","year":2000}