In vitro fertilization efficiency in coral Acropora digitifera

In vitro fertilization efficiency in coral Acropora digitifera. Iguchi, A., Morita, M., Nakajima, Y., Nishikawa, A., & Miller, D. Zygote, 17(3):225–227, August, 2009.

Paper doi abstract bibtex

Summary We performed fertilization experiments with Acropora digitifera , which is one of the dominant scleractinian corals in the Ryukyu Archipelago, Japan, to determine optimal conditions for in vitro manipulations. Our result suggests that conspecific fertilization is essentially complete within 30 min under the experimental conditions used in usual fertilization experiments in corals. Previous in vitro experiments (1 × 10 5 –10 6 sperm/ml, 4–8 h) are likely to have overestimated the efficiency of fertilization of Acropora spp. in the field. Therefore, we suggest that incubation periods shorter than those used to date (i.e. complete exclusion of sperm 1 h after their addition) would be more appropriate for the estimation of fertilization rates in corals.

@article{iguchi_vitro_2009,
	title = {\textit{{In} vitro} fertilization efficiency in coral \textit{{Acropora} digitifera}},
	volume = {17},
	issn = {0967-1994, 1469-8730},
	url = {https://www.cambridge.org/core/product/identifier/S096719940900519X/type/journal_article},
	doi = {10.1017/S096719940900519X},
	abstract = {Summary
            
              We performed fertilization experiments with
              Acropora digitifera
              , which is one of the dominant scleractinian corals in the Ryukyu Archipelago, Japan, to determine optimal conditions for
              in vitro
              manipulations. Our result suggests that conspecific fertilization is essentially complete within 30 min under the experimental conditions used in usual fertilization experiments in corals. Previous
              in vitro
              experiments (1 × 10
              5
              –10
              6
              sperm/ml, 4–8 h) are likely to have overestimated the efficiency of fertilization of
              Acropora
              spp. in the field. Therefore, we suggest that incubation periods shorter than those used to date (i.e. complete exclusion of sperm 1 h after their addition) would be more appropriate for the estimation of fertilization rates in corals.},
	language = {en},
	number = {3},
	urldate = {2021-07-27},
	journal = {Zygote},
	author = {Iguchi, Akira and Morita, Masaya and Nakajima, Yuichi and Nishikawa, Akira and Miller, David},
	month = aug,
	year = {2009},
	pages = {225--227},
}

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