Cryo Electron Tomography with Volta Phase Plate Reveals Novel Structural Foundations of the 96-Nm Axonemal Repeat in the Pathogen Trypanosoma Brucei. Imhof, S., Zhang, J., Wang, H., Bui, K. H., Nguyen, H., Atanasov, I., Hui, W. H, Yang, S. K., Zhou, Z H., & Hill, K. L eLife, 8:e52058, eLife Sciences Publications, Ltd, November, 2019. doi abstract bibtex The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from Trypanosoma brucei, a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 Å for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in T. brucei reflects adaptations to support unique motility needs in diverse host environments.
@article{imhofCryoElectronTomography2019,
title = {Cryo Electron Tomography with Volta Phase Plate Reveals Novel Structural Foundations of the 96-Nm Axonemal Repeat in the Pathogen {{Trypanosoma}} Brucei},
author = {Imhof, Simon and Zhang, Jiayan and Wang, Hui and Bui, Khanh Huy and Nguyen, Hoangkim and Atanasov, Ivo and Hui, Wong H and Yang, Shun Kai and Zhou, Z Hong and Hill, Kent L},
editor = {Carter, Andrew P and Kuriyan, John and Engel, Benjamin D and Morriswood, Brooke},
year = {2019},
month = nov,
journal = {eLife},
volume = {8},
pages = {e52058},
publisher = {eLife Sciences Publications, Ltd},
issn = {2050-084X},
doi = {10.7554/eLife.52058},
urldate = {2024-06-13},
abstract = {The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from Trypanosoma brucei, a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 {\AA} for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in T. brucei reflects adaptations to support unique motility needs in diverse host environments.},
keywords = {axoneme,cilium,dynein,motility,parasite,trypanosome},
file = {C:\Users\shervinnia\Zotero\storage\8F774EJW\Imhof et al. - 2019 - Cryo electron tomography with volta phase plate re.pdf}
}
Downloads: 0
{"_id":"axEhb54cajWuSW6bi","bibbaseid":"imhof-zhang-wang-bui-nguyen-atanasov-hui-yang-etal-cryoelectrontomographywithvoltaphaseplaterevealsnovelstructuralfoundationsofthe96nmaxonemalrepeatinthepathogentrypanosomabrucei-2019","author_short":["Imhof, S.","Zhang, J.","Wang, H.","Bui, K. H.","Nguyen, H.","Atanasov, I.","Hui, W. H","Yang, S. K.","Zhou, Z H.","Hill, K. L"],"bibdata":{"bibtype":"article","type":"article","title":"Cryo Electron Tomography with Volta Phase Plate Reveals Novel Structural Foundations of the 96-Nm Axonemal Repeat in the Pathogen Trypanosoma Brucei","author":[{"propositions":[],"lastnames":["Imhof"],"firstnames":["Simon"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Jiayan"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Hui"],"suffixes":[]},{"propositions":[],"lastnames":["Bui"],"firstnames":["Khanh","Huy"],"suffixes":[]},{"propositions":[],"lastnames":["Nguyen"],"firstnames":["Hoangkim"],"suffixes":[]},{"propositions":[],"lastnames":["Atanasov"],"firstnames":["Ivo"],"suffixes":[]},{"propositions":[],"lastnames":["Hui"],"firstnames":["Wong","H"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["Shun","Kai"],"suffixes":[]},{"propositions":[],"lastnames":["Zhou"],"firstnames":["Z","Hong"],"suffixes":[]},{"propositions":[],"lastnames":["Hill"],"firstnames":["Kent","L"],"suffixes":[]}],"editor":[{"propositions":[],"lastnames":["Carter"],"firstnames":["Andrew","P"],"suffixes":[]},{"propositions":[],"lastnames":["Kuriyan"],"firstnames":["John"],"suffixes":[]},{"propositions":[],"lastnames":["Engel"],"firstnames":["Benjamin","D"],"suffixes":[]},{"propositions":[],"lastnames":["Morriswood"],"firstnames":["Brooke"],"suffixes":[]}],"year":"2019","month":"November","journal":"eLife","volume":"8","pages":"e52058","publisher":"eLife Sciences Publications, Ltd","issn":"2050-084X","doi":"10.7554/eLife.52058","urldate":"2024-06-13","abstract":"The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from Trypanosoma brucei, a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 Å for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in T. brucei reflects adaptations to support unique motility needs in diverse host environments.","keywords":"axoneme,cilium,dynein,motility,parasite,trypanosome","file":"C:\\Users\\shervinnia\\Zotero\\storage\\8F774EJW\\Imhof et al. - 2019 - Cryo electron tomography with volta phase plate re.pdf","bibtex":"@article{imhofCryoElectronTomography2019,\n title = {Cryo Electron Tomography with Volta Phase Plate Reveals Novel Structural Foundations of the 96-Nm Axonemal Repeat in the Pathogen {{Trypanosoma}} Brucei},\n author = {Imhof, Simon and Zhang, Jiayan and Wang, Hui and Bui, Khanh Huy and Nguyen, Hoangkim and Atanasov, Ivo and Hui, Wong H and Yang, Shun Kai and Zhou, Z Hong and Hill, Kent L},\n editor = {Carter, Andrew P and Kuriyan, John and Engel, Benjamin D and Morriswood, Brooke},\n year = {2019},\n month = nov,\n journal = {eLife},\n volume = {8},\n pages = {e52058},\n publisher = {eLife Sciences Publications, Ltd},\n issn = {2050-084X},\n doi = {10.7554/eLife.52058},\n urldate = {2024-06-13},\n abstract = {The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from Trypanosoma brucei, a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 {\\AA} for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in T. brucei reflects adaptations to support unique motility needs in diverse host environments.},\n keywords = {axoneme,cilium,dynein,motility,parasite,trypanosome},\n file = {C:\\Users\\shervinnia\\Zotero\\storage\\8F774EJW\\Imhof et al. - 2019 - Cryo electron tomography with volta phase plate re.pdf}\n}\n\n","author_short":["Imhof, S.","Zhang, J.","Wang, H.","Bui, K. H.","Nguyen, H.","Atanasov, I.","Hui, W. H","Yang, S. K.","Zhou, Z H.","Hill, K. L"],"editor_short":["Carter, A. P","Kuriyan, J.","Engel, B. D","Morriswood, B."],"key":"imhofCryoElectronTomography2019","id":"imhofCryoElectronTomography2019","bibbaseid":"imhof-zhang-wang-bui-nguyen-atanasov-hui-yang-etal-cryoelectrontomographywithvoltaphaseplaterevealsnovelstructuralfoundationsofthe96nmaxonemalrepeatinthepathogentrypanosomabrucei-2019","role":"author","urls":{},"keyword":["axoneme","cilium","dynein","motility","parasite","trypanosome"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/2MtabhipmJFGQ7n9n","dataSources":["mrv2RSxFMmgGBMu4y"],"keywords":["axoneme","cilium","dynein","motility","parasite","trypanosome"],"search_terms":["cryo","electron","tomography","volta","phase","plate","reveals","novel","structural","foundations","axonemal","repeat","pathogen","trypanosoma","brucei","imhof","zhang","wang","bui","nguyen","atanasov","hui","yang","zhou","hill"],"title":"Cryo Electron Tomography with Volta Phase Plate Reveals Novel Structural Foundations of the 96-Nm Axonemal Repeat in the Pathogen Trypanosoma Brucei","year":2019}