@article{simura_plant_2018,
	title = {Plant {Hormonomics}: {Multiple} {Phytohormone} {Profiling} by {Targeted} {Metabolomics}},
	volume = {177},
	issn = {1532-2548},
	shorttitle = {Plant {Hormonomics}},
	url = {https://academic.oup.com/plphys/article/177/2/476/6117035},
	doi = {10/gdrpsw},
	abstract = {Abstract
            Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.},
	language = {en},
	number = {2},
	urldate = {2021-06-07},
	journal = {Plant Physiology},
	author = {Šimura, Jan and Antoniadi, Ioanna and Široká, Jitka and Tarkowská, Danu¡e and Strnad, Miroslav and Ljung, Karin and Novák, Ondřej},
	month = jun,
	year = {2018},
	pages = {476--489},
}

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