@ARTICLE{Isaev-Ivanov1983362,
author={Isaev-Ivanov, V.V. and Isaeva-Ivanova, L.S. and Kleǐner, A.R. and Odintsov, V.B. and Sidorov, O.I.},
title={Spectral division of conformational states of spin-labeled tRNA Phe from Escherichia coli [Spektral'noe razdelenie konformatsionnykh sostoianiǐ na spin-mechenoǐ tRNK Phe iz Escherichia coli.]},
journal={Molekulyarnaya Biologiya},
year={1983},
volume={17},
number={2},
pages={362-372},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020724858&partnerID=40&md5=2ed571f6fdc7bf24bfde8271a0fd7fec},
abstract={A spectral division method of two conformational states of spin-labeled macromolecules is presented. The method is suitable in conditions of highly anisotropic motion of spin label and is based on titration of experimental spectra of spin-labeled macromolecule by theoretical ones. Theoretical spectra simulation uses the Freed theory and spin-Hamiltonian parameters, derived from independent experiments. Nomogrammes and formula for calculation of order parameter Sz and correlation time tau c in temperature-viscosity experiment are available. The method was applied to spectral division of two conformational states of spin-labeled tRNAPhe from E. coli and spectral parameters Sz and tau c were obtained for both states. ESR spectra of these conformational states at t degree = 20 degrees differ strongly from one another by order parameter Sz. The first conformer, that is characterised by a greater order parameter has no globular conformational transition (in terms of changes of the hydrodynamic macromolecule radius) between 2 degrees and 20 degrees, but local conformational changes take place in this temperature region.},
correspondence_address1={Isaev-Ivanov, V.V.},
issn={00268984},
pubmed_id={6304493},
language={Russian},
abbrev_source_title={Mol Biol (Mosk)},
document_type={Article},
source={Scopus},
}

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The method is suitable in conditions of highly anisotropic motion of spin label and is based on titration of experimental spectra of spin-labeled macromolecule by theoretical ones. Theoretical spectra simulation uses the Freed theory and spin-Hamiltonian parameters, derived from independent experiments. Nomogrammes and formula for calculation of order parameter Sz and correlation time tau c in temperature-viscosity experiment are available. The method was applied to spectral division of two conformational states of spin-labeled tRNAPhe from E. coli and spectral parameters Sz and tau c were obtained for both states. ESR spectra of these conformational states at t degree = 20 degrees differ strongly from one another by order parameter Sz. The first conformer, that is characterised by a greater order parameter has no globular conformational transition (in terms of changes of the hydrodynamic macromolecule radius) between 2 degrees and 20 degrees, but local conformational changes take place in this temperature region.","correspondence_address1":"Isaev-Ivanov, V.V.","issn":"00268984","pubmed_id":"6304493","language":"Russian","abbrev_source_title":"Mol Biol (Mosk)","document_type":"Article","source":"Scopus","bibtex":"@ARTICLE{Isaev-Ivanov1983362,\r\nauthor={Isaev-Ivanov, V.V. and Isaeva-Ivanova, L.S. and Kleǐner, A.R. and Odintsov, V.B. and Sidorov, O.I.},\r\ntitle={Spectral division of conformational states of spin-labeled tRNA Phe from Escherichia coli [Spektral'noe razdelenie konformatsionnykh sostoianiǐ na spin-mechenoǐ tRNK Phe iz Escherichia coli.]},\r\njournal={Molekulyarnaya Biologiya},\r\nyear={1983},\r\nvolume={17},\r\nnumber={2},\r\npages={362-372},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020724858&partnerID=40&md5=2ed571f6fdc7bf24bfde8271a0fd7fec},\r\nabstract={A spectral division method of two conformational states of spin-labeled macromolecules is presented. The method is suitable in conditions of highly anisotropic motion of spin label and is based on titration of experimental spectra of spin-labeled macromolecule by theoretical ones. Theoretical spectra simulation uses the Freed theory and spin-Hamiltonian parameters, derived from independent experiments. Nomogrammes and formula for calculation of order parameter Sz and correlation time tau c in temperature-viscosity experiment are available. The method was applied to spectral division of two conformational states of spin-labeled tRNAPhe from E. coli and spectral parameters Sz and tau c were obtained for both states. ESR spectra of these conformational states at t degree = 20 degrees differ strongly from one another by order parameter Sz. The first conformer, that is characterised by a greater order parameter has no globular conformational transition (in terms of changes of the hydrodynamic macromolecule radius) between 2 degrees and 20 degrees, but local conformational changes take place in this temperature region.},\r\ncorrespondence_address1={Isaev-Ivanov, V.V.},\r\nissn={00268984},\r\npubmed_id={6304493},\r\nlanguage={Russian},\r\nabbrev_source_title={Mol Biol (Mosk)},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n","author_short":["Isaev-Ivanov, V.","Isaeva-Ivanova, L.","Kleǐner, A.","Odintsov, V.","Sidorov, O."],"key":"Isaev-Ivanov1983362","id":"Isaev-Ivanov1983362","bibbaseid":"isaevivanov-isaevaivanova-klener-odintsov-sidorov-spectraldivisionofconformationalstatesofspinlabeledtrnaphefromescherichiacolispektralnoerazdeleniekonformatsionnykhsostoianinaspinmechenotrnkpheizescherichiacoli-1983","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0020724858&partnerID=40&md5=2ed571f6fdc7bf24bfde8271a0fd7fec"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lbm_2019_10.txt","dataSources":["YYLjupmCazkNqWNEJ"],"keywords":[],"search_terms":["spectral","division","conformational","states","spin","labeled","trna","phe","escherichia","coli","spektral","noe","razdelenie","konformatsionnykh","sostoiani","spin","mecheno","trnk","phe","escherichia","coli","isaev-ivanov","isaeva-ivanova","kleǐner","odintsov","sidorov"],"title":"Spectral division of conformational states of spin-labeled tRNA Phe from Escherichia coli [Spektral'noe razdelenie konformatsionnykh sostoianiǐ na spin-mechenoǐ tRNK Phe iz Escherichia coli.]","year":1983}