Identification and Biochemical Characterization of anArabidopsis Indole-3-acetic Acid Glucosyltransferase*

Identification and Biochemical Characterization of anArabidopsis Indole-3-acetic Acid Glucosyltransferase*. Jackson, R. G., Lim, E., Li, Y., Kowalczyk, M., Sandberg, G., Hoggett, J., Ashford, D. A., & Bowles, D. J. Journal of Biological Chemistry, 276(6):4350–4356, February, 2001.

Paper doi abstract bibtex

Biochemical characterization of recombinant gene products following a phylogenetic analysis of the UDP-glucosyltransferase (UGT) multigene family ofArabidopsis has identified one enzyme (UGT84B1) with high activity toward the plant hormone indole-3-acetic acid (IAA) and three related enzymes (UGT84B2, UGT75B1, and UGT75B2) with trace activities. The identity of the IAA conjugate has been confirmed to be 1-O-indole acetyl glucose ester. A sequence annotated as a UDP-glucose:IAA glucosyltransferase (IAA-UGT) in theArabidopsis genome and expressed sequence tag data bases given its similarity to the maize iaglu gene sequence showed no activity toward IAA. This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester inArabidopsis.

@article{jackson_identification_2001,
	title = {Identification and {Biochemical} {Characterization} of {anArabidopsis} {Indole}-3-acetic {Acid} {Glucosyltransferase}*},
	volume = {276},
	issn = {0021-9258},
	url = {https://www.sciencedirect.com/science/article/pii/S0021925818464423},
	doi = {10/d3cc4r},
	abstract = {Biochemical characterization of recombinant gene products following a phylogenetic analysis of the UDP-glucosyltransferase (UGT) multigene family ofArabidopsis has identified one enzyme (UGT84B1) with high activity toward the plant hormone indole-3-acetic acid (IAA) and three related enzymes (UGT84B2, UGT75B1, and UGT75B2) with trace activities. The identity of the IAA conjugate has been confirmed to be 1-O-indole acetyl glucose ester. A sequence annotated as a UDP-glucose:IAA glucosyltransferase (IAA-UGT) in theArabidopsis genome and expressed sequence tag data bases given its similarity to the maize iaglu gene sequence showed no activity toward IAA. This study describes the first biochemical analysis of a recombinant IAA-UGT and provides the foundation for future genetic approaches to understand the role of 1-O-indole acetyl glucose ester inArabidopsis.},
	language = {en},
	number = {6},
	urldate = {2021-11-02},
	journal = {Journal of Biological Chemistry},
	author = {Jackson, Rosamond G. and Lim, Eng-Kiat and Li, Yi and Kowalczyk, Mariusz and Sandberg, Göran and Hoggett, Jim and Ashford, David A. and Bowles, Dianna J.},
	month = feb,
	year = {2001},
	pages = {4350--4356},
}

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