Engineering high post-electroporation viabilities and transfection efficiencies for elongated cells on suspended nanofiber networks

Engineering high post-electroporation viabilities and transfection efficiencies for elongated cells on suspended nanofiber networks. Jacobs, E. J., Graybill, P. M., Jana, A., Agashe, A., Nain, A. S., & Davalos, R. V. BIOELECTROCHEMISTRY, ELSEVIER SCIENCE SA, PO BOX 564, 1001 LAUSANNE, SWITZERLAND, 2023 AUG, 2023.
doi abstract bibtex

The impact of cell shape on cell membrane permeabilization by pulsed electric fields is not fully understood. For certain applications, cell survival and recovery post-treatment is either desirable, as in gene transfection, electrofusion, and electrochemotherapy, or is undesirable, as in tumor and cardiac ablations. Understanding of how morphology affects cell viability post-electroporation may lead to improved electroporation methods. In this study, we use precisely aligned nanofiber networks within a microfluidic device to reproducibly generate elongated cells with controlled orientations to an applied electric field. We show that cell viability is significantly dependent on cell orientation, elongation, and spread. Further, these trends are dependent on the external buffer conductivity. Additionally, we see that cell survival for elongated cells is still supported by the standard pore model of electroporation. Lastly, we see that manipulating the cell orientation and shape can be leveraged for increased transfection efficiencies when compared to spherical cells. An improved understanding of cell shape and pulsation buffer conductivity may lead to improved methods for enhancing cell viability post-electroporation by engineering the cell morphology, cytoskeleton, and electroporation buffer conditions.

@article{ WOS:000967527900001,
Author = {Jacobs, Edward J. and Graybill, Philip M. and Jana, Aniket and Agashe,
   Atharva and Nain, Amrinder S. and Davalos, Rafael V.},
Title = {Engineering high post-electroporation viabilities and transfection
   efficiencies for elongated cells on suspended nanofiber networks},
Journal = {BIOELECTROCHEMISTRY},
Year = {2023},
Volume = {152},
Month = {2023 AUG},
Abstract = {The impact of cell shape on cell membrane permeabilization by pulsed
   electric fields is not fully understood. For certain applications, cell
   survival and recovery post-treatment is either desirable, as in gene
   transfection, electrofusion, and electrochemotherapy, or is undesirable,
   as in tumor and cardiac ablations. Understanding of how morphology
   affects cell viability post-electroporation may lead to improved
   electroporation methods. In this study, we use precisely aligned
   nanofiber networks within a microfluidic device to reproducibly generate
   elongated cells with controlled orientations to an applied electric
   field. We show that cell viability is significantly dependent on cell
   orientation, elongation, and spread. Further, these trends are dependent
   on the external buffer conductivity. Additionally, we see that cell
   survival for elongated cells is still supported by the standard pore
   model of electroporation. Lastly, we see that manipulating the cell
   orientation and shape can be leveraged for increased transfection
   efficiencies when compared to spherical cells. An improved understanding
   of cell shape and pulsation buffer conductivity may lead to improved
   methods for enhancing cell viability post-electroporation by engineering
   the cell morphology, cytoskeleton, and electroporation buffer
   conditions.},
Publisher = {ELSEVIER SCIENCE SA},
Address = {PO BOX 564, 1001 LAUSANNE, SWITZERLAND},
Type = {Article; Early Access},
Language = {English},
Affiliation = {Davalos, RV (Corresponding Author), Virginia Tech, Dept Biomed Engn \& Mech, Blacksburg, VA 24061 USA.
   Nain, AS (Corresponding Author), Virginia Tech, Dept Mech Engn, Blacksburg, VA 24061 USA.
   Jacobs, Edward J.; Davalos, Rafael V., Virginia Tech, Dept Biomed Engn \& Mech, Blacksburg, VA 24061 USA.
   Graybill, Philip M.; Jana, Aniket; Agashe, Atharva; Nain, Amrinder S., Virginia Tech, Dept Mech Engn, Blacksburg, VA 24061 USA.},
DOI = {10.1016/j.bioelechem.2023.108415},
EarlyAccessDate = {APR 2023},
Article-Number = {108415},
ISSN = {1567-5394},
EISSN = {1878-562X},
Keywords = {Pulsed Electric Fields; Electroporation; Nanofibers; Viability;
   Transfection; Cytoskeleton},
Keywords-Plus = {IRREVERSIBLE ELECTROPORATION; THEORETICAL-ANALYSIS; MAMMALIAN-CELLS;
   SINGLE; ELECTROPERMEABILIZATION; ELECTROCHEMOTHERAPY; CONDUCTIVITY;
   ABLATION},
Research-Areas = {Biochemistry \& Molecular Biology; Life Sciences \& Biomedicine - Other
   Topics; Biophysics; Electrochemistry},
Web-of-Science-Categories  = {Biochemistry \& Molecular Biology; Biology; Biophysics; Electrochemistry},
Author-Email = {nain@vt.edu
   davalos@vt.edu},
Affiliations = {Virginia Polytechnic Institute \& State University; Virginia Polytechnic
   Institute \& State University},
ResearcherID-Numbers = {Graybill, Philip M/K-7651-2017},
Funding-Acknowledgement = {NIH PO1 {[}PO1CA207206]; National Science Foundation {[}1762634,
   2119949]; Spinneret-based Tunable Engineered Parameters (STEP) Lab,
   Virginia Tech; Institute of Critical Technologies and Sciences (ICTAS);
   Macromolecules Innovation Institute at Virginia Tech},
Funding-Text = {This research was funded in part by the NIH PO1 (PO1CA207206) and
   completed through the collaboration between the BEMS lab and STEP lab at
   Virginia Tech. A.S.N. acknowledges support from the National Science
   Foundation (grants 1762634 and 2119949). The opinions, findings, and
   conclusions, or recommendations expressed are those of the author(s) and
   do not necessarily reflect the views of the National Science Foundation.
   A.S.N. acknowledges support from members of the Spinneret-based Tunable
   Engineered Parameters (STEP) Lab, Virginia Tech, and thanks the
   Institute of Critical Technologies and Sciences (ICTAS) and
   Macromolecules Innovation Institute at Virginia Tech for the support to
   conduct this study.},
Number-of-Cited-References = {59},
Times-Cited = {2},
Usage-Count-Last-180-days = {5},
Usage-Count-Since-2013 = {11},
Journal-ISO = {Bioelectrochemistry},
Doc-Delivery-Number = {D3CD5},
Web-of-Science-Index = {Science Citation Index Expanded (SCI-EXPANDED)},
Unique-ID = {WOS:000967527900001},
DA = {2024-03-03},
}

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