@article{RN96,
   author = {Jacobs Iv, E. J. and Graybill, P. M. and Jana, A. and Agashe, A. and Nain, A. S. and Davalos, R. V.},
   title = {Engineering high post-electroporation viabilities and transfection efficiencies for elongated cells on suspended nanofiber networks},
   journal = {Bioelectrochemistry},
   volume = {152},
   pages = {108415},
   note = {1878-562x
Jacobs Iv, Edward J
Graybill, Philip M
Jana, Aniket
Agashe, Atharva
Nain, Amrinder S
Davalos, Rafael V
Journal Article
Netherlands
2023/04/04
Bioelectrochemistry. 2023 Aug;152:108415. doi: 10.1016/j.bioelechem.2023.108415. Epub 2023 Mar 21.},
   abstract = {The impact of cell shape on cell membrane permeabilization by pulsed electric fields is not fully understood. For certain applications, cell survival and recovery post-treatment is either desirable, as in gene transfection, electrofusion, and electrochemotherapy, or is undesirable, as in tumor and cardiac ablations. Understanding of how morphology affects cell viability post-electroporation may lead to improved electroporation methods. In this study, we use precisely aligned nanofiber networks within a microfluidic device to reproducibly generate elongated cells with controlled orientations to an applied electric field. We show that cell viability is significantly dependent on cell orientation, elongation, and spread. Further, these trends are dependent on the external buffer conductivity. Additionally, we see that cell survival for elongated cells is still supported by the standard pore model of electroporation. Lastly, we see that manipulating the cell orientation and shape can be leveraged for increased transfection efficiencies when compared to spherical cells. An improved understanding of cell shape and pulsation buffer conductivity may lead to improved methods for enhancing cell viability post-electroporation by engineering the cell morphology, cytoskeleton, and electroporation buffer conditions.},
   keywords = {Humans
*Nanofibers
Electroporation/methods
Transfection
*Electrochemotherapy/methods
*Neoplasms
Cell Survival
Cytoskeleton
Electroporation
Nanofibers
Pulsed Electric Fields
Viability},
   ISSN = {1567-5394},
   DOI = {10.1016/j.bioelechem.2023.108415},
   year = {2023},
   type = {Journal Article}
}

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V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Jacobs","Iv"],"firstnames":["E.","J."],"suffixes":[]},{"propositions":[],"lastnames":["Graybill"],"firstnames":["P.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Jana"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Agashe"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Nain"],"firstnames":["A.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Engineering high post-electroporation viabilities and transfection efficiencies for elongated cells on suspended nanofiber networks","journal":"Bioelectrochemistry","volume":"152","pages":"108415","note":"1878-562x Jacobs Iv, Edward J Graybill, Philip M Jana, Aniket Agashe, Atharva Nain, Amrinder S Davalos, Rafael V Journal Article Netherlands 2023/04/04 Bioelectrochemistry. 2023 Aug;152:108415. doi: 10.1016/j.bioelechem.2023.108415. Epub 2023 Mar 21.","abstract":"The impact of cell shape on cell membrane permeabilization by pulsed electric fields is not fully understood. For certain applications, cell survival and recovery post-treatment is either desirable, as in gene transfection, electrofusion, and electrochemotherapy, or is undesirable, as in tumor and cardiac ablations. Understanding of how morphology affects cell viability post-electroporation may lead to improved electroporation methods. In this study, we use precisely aligned nanofiber networks within a microfluidic device to reproducibly generate elongated cells with controlled orientations to an applied electric field. We show that cell viability is significantly dependent on cell orientation, elongation, and spread. Further, these trends are dependent on the external buffer conductivity. Additionally, we see that cell survival for elongated cells is still supported by the standard pore model of electroporation. Lastly, we see that manipulating the cell orientation and shape can be leveraged for increased transfection efficiencies when compared to spherical cells. An improved understanding of cell shape and pulsation buffer conductivity may lead to improved methods for enhancing cell viability post-electroporation by engineering the cell morphology, cytoskeleton, and electroporation buffer conditions.","keywords":"Humans *Nanofibers Electroporation/methods Transfection *Electrochemotherapy/methods *Neoplasms Cell Survival Cytoskeleton Electroporation Nanofibers Pulsed Electric Fields Viability","issn":"1567-5394","doi":"10.1016/j.bioelechem.2023.108415","year":"2023","bibtex":"@article{RN96,\n author = {Jacobs Iv, E. J. and Graybill, P. M. and Jana, A. and Agashe, A. and Nain, A. S. and Davalos, R. V.},\n title = {Engineering high post-electroporation viabilities and transfection efficiencies for elongated cells on suspended nanofiber networks},\n journal = {Bioelectrochemistry},\n volume = {152},\n pages = {108415},\n note = {1878-562x\nJacobs Iv, Edward J\nGraybill, Philip M\nJana, Aniket\nAgashe, Atharva\nNain, Amrinder S\nDavalos, Rafael V\nJournal Article\nNetherlands\n2023/04/04\nBioelectrochemistry. 2023 Aug;152:108415. doi: 10.1016/j.bioelechem.2023.108415. Epub 2023 Mar 21.},\n abstract = {The impact of cell shape on cell membrane permeabilization by pulsed electric fields is not fully understood. For certain applications, cell survival and recovery post-treatment is either desirable, as in gene transfection, electrofusion, and electrochemotherapy, or is undesirable, as in tumor and cardiac ablations. Understanding of how morphology affects cell viability post-electroporation may lead to improved electroporation methods. In this study, we use precisely aligned nanofiber networks within a microfluidic device to reproducibly generate elongated cells with controlled orientations to an applied electric field. We show that cell viability is significantly dependent on cell orientation, elongation, and spread. Further, these trends are dependent on the external buffer conductivity. Additionally, we see that cell survival for elongated cells is still supported by the standard pore model of electroporation. Lastly, we see that manipulating the cell orientation and shape can be leveraged for increased transfection efficiencies when compared to spherical cells. 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V."],"key":"RN96","id":"RN96","bibbaseid":"jacobsiv-graybill-jana-agashe-nain-davalos-engineeringhighpostelectroporationviabilitiesandtransfectionefficienciesforelongatedcellsonsuspendednanofibernetworks-2023","role":"author","urls":{},"keyword":["Humans *Nanofibers Electroporation/methods Transfection *Electrochemotherapy/methods *Neoplasms Cell Survival Cytoskeleton Electroporation Nanofibers Pulsed Electric Fields Viability"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["humans *nanofibers electroporation/methods transfection *electrochemotherapy/methods *neoplasms cell survival cytoskeleton electroporation nanofibers pulsed electric fields viability"],"search_terms":["engineering","high","post","electroporation","viabilities","transfection","efficiencies","elongated","cells","suspended","nanofiber","networks","jacobs iv","graybill","jana","agashe","nain","davalos"],"title":"Engineering high post-electroporation viabilities and transfection efficiencies for elongated cells on suspended nanofiber networks","year":2023}