Genes in museum collections. Jaksch, K., Eschner, A., & Haring, E. In Johnsbach, Austria, August, 2014.
abstract   bibtex   
Museums harbour huge treasuries of new or old, regular or odd, common or rare animals – and therefore also a big collection of genes. This is quite vital as the molecular genetic approach in scientific studies is very important, but sometimes it is not possible to gain fresh material, because the animal is e.g. extinct nowadays, difficult to find or not native in a special area anymore. In the framework of a Synthesys 2 - JRA 5 project, we tested the usage of museum material for DNA-analyses in molluscs with two different extraction methods. In total we analysed specimens from 72 glasses harboured in our mollusc collection. comprising 20 different taxa of 4 classes. The years of collection of the chosen samples ranged from 1877 to 2002. To test the success of the DNA extraction, we amplified two short sections of the mitochondrial genome (COI and 16S rRNA genes). The gastropod taxa worked best with all tested primer sets, although the slug taxa yielded mostly bad results. The polyplacophors showed good results, the bivalves and the cephalopods worked sparsely. Concerning the age a higher percentage of the samples collected after 1900 showed positive results than the samples before 1900. Nevertheless, several samples aged about 120 years worked well, which is a quite surprising and important outcome of the project.
@inproceedings{jaksch_genes_2014,
	address = {Johnsbach, Austria},
	title = {Genes in museum collections},
	abstract = {Museums harbour huge treasuries of new or old, regular or odd, common or rare animals – and therefore also a big collection of genes. This is quite vital as the molecular genetic approach in scientific studies is very important, but sometimes it is not possible to gain fresh material, because the animal is e.g. extinct nowadays, difficult to find or not native in a special area anymore. In the framework of a Synthesys 2 - JRA 5 project, we tested the usage of museum material for DNA-analyses in molluscs with two different extraction methods. In total we analysed specimens from 72 glasses harboured in our mollusc collection. comprising 20 different taxa of 4 classes. The years of collection of the chosen samples ranged from 1877 to 2002. To test the success of the DNA extraction, we amplified two short sections of the mitochondrial genome (COI and 16S rRNA genes).
The gastropod taxa worked best with all tested primer sets, although the slug taxa yielded mostly bad results. The polyplacophors showed good results, the bivalves and the cephalopods worked sparsely. Concerning the age a higher percentage of the samples collected after 1900 showed positive results than the samples before 1900. Nevertheless, several samples aged about 120 years worked well, which is a quite surprising and important outcome of the project.},
	language = {English},
	author = {Jaksch, Katharina and Eschner, Anita and Haring, Elisabeth},
	month = aug,
	year = {2014},
}

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