@article{Jalani2015473,
  abstract = {Injectable hydrogels are extensively used in drug delivery and tissue engineering to administer drugs, genes, growth factors and live cells. We report a method to produce tough, in-situ thermogelling, non-toxic, injectable hydrogels made of chitosan and hyaluronic acid co-crosslinked with carboxytetramethylrhodamine-glycerophophate and genipin. The gels are highly homogeneous and form within 32 min, i.e., faster than gels crosslinked with either genipin or beta-glycerophophate. The shear strength of co-crosslinked hydrogels is 3.5 kPa, higher than any chitosan-based gel reported. Chondrocytes and nucleus pulposus cells thrive inside the gels and produce large amounts of collagen II. Injection in rats shows that the gels form in-vivo within a short time and remain well localized for more than one week while the rats remain healthy and active. The excellent mechanical properties, fast in-situ gelation, good biocompatibility and the ability to encapsulate live cells at physiological conditions make these hydrogels ideal for tissue engineering, especially cartilage regeneration. Homogenous, insitu thermogelling, highly tough, non-toxic injectable hydrogels from chitosan and hyaluronic acid co-crosslinked with beta-glycerophophate and genipin are produced. The co-crosslinked gels form faster than genipin-only crosslinked gels and are 2.5 times stronger than ionically crosslinked gels. Homogenously dispersed cells express enhanced amount of collagen II, showing the great potential of these gels for cartilage tissue engineering.},
  annote = {cited By 15},
  author = {Jalani, Ghulam and Rosenzweig, Derek H. and Makhoul, Georges and Abdalla, Sherif and Cecere, Renzo and Vetrone, Fiorenzo and Haglund, Lisbet and Cerruti, Marta},
  doi = {10.1002/mabi.201400406},
  issn = {16165195},
  journal = {Macromolecular Bioscience},
  keywords = {crosslinking,genipin,hydrogels,in-situ thermogelling,injectable},
  number = {4},
  pages = {473--480},
  title = {{Tough, in-situ thermogelling, injectable hydrogels for biomedical applications}},
  volume = {15},
  year = {2015}
  }