@article{RN241,
   author = {James, C. D. and Reuel, N. and Lee, E. S. and Davalos, R. V. and Mani, S. S. and Carroll-Portillo, A. and Rebeil, R. and Martino, A. and Apblett, C. A.},
   title = {Impedimetric and optical interrogation of single cells in a microfluidic device for real-time viability and chemical response assessment},
   journal = {Biosens Bioelectron},
   volume = {23},
   number = {6},
   pages = {845-51},
   note = {James, Conrad D
Reuel, Nigel
Lee, Eunice S
Davalos, Rafael V
Mani, Seethambal S
Carroll-Portillo, Amanda
Rebeil, Roberto
Martino, Anthony
Apblett, Christopher A
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
England
2007/10/16
Biosens Bioelectron. 2008 Jan 18;23(6):845-51. doi: 10.1016/j.bios.2007.08.022. Epub 2007 Sep 6.},
   abstract = {We report here a non-invasive, reversible method for interrogating single cells in a microfluidic flow-through system. Impedance spectroscopy of cells held at a micron-sized pore under negative pressure is demonstrated and used to determine the presence and viability of the captured cell. The cell capture pore is optimized for electrical response and mechanical interfacing to a cell using a deposited layer of parylene. Changes in the mechanical interface between the cell and the chip due to chemical exposure or environmental changes can also be assessed. Here, we monitored the change in adhesion/spreading of RAW264.7 macrophages in response to the immune stimulant lipopolysaccharide (LPS). This method enables selective, reversible, and quantitative long-term impedance measurements on single cells. The fully sealed electrofluidic assembly is compatible with long-term cell culturing, and could be modified to incorporate single cell lysis and subsequent intracellular separation and analysis.},
   keywords = {Cell Survival
Cells, Cultured
Electric Impedance
Lipopolysaccharides/pharmacology
Macrophages/drug effects/*physiology
*Microfluidic Analytical Techniques
Optics and Photonics
Spectrum Analysis},
   ISSN = {0956-5663 (Print)
0956-5663},
   DOI = {10.1016/j.bios.2007.08.022},
   year = {2008},
   type = {Journal Article}
}

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A."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["James"],"firstnames":["C.","D."],"suffixes":[]},{"propositions":[],"lastnames":["Reuel"],"firstnames":["N."],"suffixes":[]},{"propositions":[],"lastnames":["Lee"],"firstnames":["E.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Mani"],"firstnames":["S.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Carroll-Portillo"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Rebeil"],"firstnames":["R."],"suffixes":[]},{"propositions":[],"lastnames":["Martino"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Apblett"],"firstnames":["C.","A."],"suffixes":[]}],"title":"Impedimetric and optical interrogation of single cells in a microfluidic device for real-time viability and chemical response assessment","journal":"Biosens Bioelectron","volume":"23","number":"6","pages":"845-51","note":"James, Conrad D Reuel, Nigel Lee, Eunice S Davalos, Rafael V Mani, Seethambal S Carroll-Portillo, Amanda Rebeil, Roberto Martino, Anthony Apblett, Christopher A Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2007/10/16 Biosens Bioelectron. 2008 Jan 18;23(6):845-51. doi: 10.1016/j.bios.2007.08.022. Epub 2007 Sep 6.","abstract":"We report here a non-invasive, reversible method for interrogating single cells in a microfluidic flow-through system. Impedance spectroscopy of cells held at a micron-sized pore under negative pressure is demonstrated and used to determine the presence and viability of the captured cell. The cell capture pore is optimized for electrical response and mechanical interfacing to a cell using a deposited layer of parylene. Changes in the mechanical interface between the cell and the chip due to chemical exposure or environmental changes can also be assessed. Here, we monitored the change in adhesion/spreading of RAW264.7 macrophages in response to the immune stimulant lipopolysaccharide (LPS). This method enables selective, reversible, and quantitative long-term impedance measurements on single cells. The fully sealed electrofluidic assembly is compatible with long-term cell culturing, and could be modified to incorporate single cell lysis and subsequent intracellular separation and analysis.","keywords":"Cell Survival Cells, Cultured Electric Impedance Lipopolysaccharides/pharmacology Macrophages/drug effects/*physiology *Microfluidic Analytical Techniques Optics and Photonics Spectrum Analysis","issn":"0956-5663 (Print) 0956-5663","doi":"10.1016/j.bios.2007.08.022","year":"2008","bibtex":"@article{RN241,\n author = {James, C. D. and Reuel, N. and Lee, E. S. and Davalos, R. V. and Mani, S. S. and Carroll-Portillo, A. and Rebeil, R. and Martino, A. and Apblett, C. A.},\n title = {Impedimetric and optical interrogation of single cells in a microfluidic device for real-time viability and chemical response assessment},\n journal = {Biosens Bioelectron},\n volume = {23},\n number = {6},\n pages = {845-51},\n note = {James, Conrad D\nReuel, Nigel\nLee, Eunice S\nDavalos, Rafael V\nMani, Seethambal S\nCarroll-Portillo, Amanda\nRebeil, Roberto\nMartino, Anthony\nApblett, Christopher A\nJournal Article\nResearch Support, Non-U.S. Gov't\nResearch Support, U.S. Gov't, Non-P.H.S.\nEngland\n2007/10/16\nBiosens Bioelectron. 2008 Jan 18;23(6):845-51. doi: 10.1016/j.bios.2007.08.022. Epub 2007 Sep 6.},\n abstract = {We report here a non-invasive, reversible method for interrogating single cells in a microfluidic flow-through system. Impedance spectroscopy of cells held at a micron-sized pore under negative pressure is demonstrated and used to determine the presence and viability of the captured cell. The cell capture pore is optimized for electrical response and mechanical interfacing to a cell using a deposited layer of parylene. Changes in the mechanical interface between the cell and the chip due to chemical exposure or environmental changes can also be assessed. Here, we monitored the change in adhesion/spreading of RAW264.7 macrophages in response to the immune stimulant lipopolysaccharide (LPS). This method enables selective, reversible, and quantitative long-term impedance measurements on single cells. The fully sealed electrofluidic assembly is compatible with long-term cell culturing, and could be modified to incorporate single cell lysis and subsequent intracellular separation and analysis.},\n keywords = {Cell Survival\nCells, Cultured\nElectric Impedance\nLipopolysaccharides/pharmacology\nMacrophages/drug effects/*physiology\n*Microfluidic Analytical Techniques\nOptics and Photonics\nSpectrum Analysis},\n ISSN = {0956-5663 (Print)\n0956-5663},\n DOI = {10.1016/j.bios.2007.08.022},\n year = {2008},\n type = {Journal Article}\n}\n\n","author_short":["James, C. D.","Reuel, N.","Lee, E. S.","Davalos, R. V.","Mani, S. S.","Carroll-Portillo, A.","Rebeil, R.","Martino, A.","Apblett, C. 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