@article{jang_rutin_2014,
	title = {Rutin improves functional outcome via reducing the elevated matrix metalloproteinase-9 level in a photothrombotic focal ischemic model of rats},
	volume = {339},
	issn = {1878-5883},
	doi = {10.1016/j.jns.2014.01.024},
	abstract = {BACKGROUND: Blood-brain barrier (BBB) disruption mediated by proteases plays a pivotal role in neural tissue damage after acute ischemic stroke. In an animal stroke model, the activation of matrix metalloproteinases (MMPs), especially MMP-9, was significantly increased and it showed potential association with blood-brain barrier (BBB) disruption and cerebral edema. Theoretically, it is expected that early blockade of expression and activation of MMP-9 after ischemic stroke provides neuroprotective effects from secondary neural tissue damage. This study was aimed to determine the ability of rutin to influence MMP-9 expression, activity and BBB disruption using a photothrombotic focal ischemic model in rats.
METHODS: Adult male Sprague-Dawley rats, weighing between 250 and 300 g (aged 8 weeks) received focal cerebral ischemia by photothrombosis using Rose Bengal (RB) and cold light. Injured animals were divided into two groups; one group received 50mg/kg of rutin intraperitoneally, starting 1h after injury and at 12h intervals for 3 days, while animals in the control group received weight-adjusted doses of saline vehicle over the same period. In each group, the expressions and activities of MMP-9 were assessed by Western blot and gelatin zymography at 6, 24, 48, and 72 h after photothrombotic insult. The effects of rutin on BBB disruption and functional outcomes were also determined.
RESULTS: Western blot and zymographic analysis showed up-regulated MMP-9 expression and activity in the ischemic cortex. The expression and activity of MMP-9 were significantly elevated at 6h after photothrombotic insult, which remained up-regulated for at least until 72 h after injury. In the rutin-treated group, MMP-9 expression and activity were significantly attenuated at 6, 24, and 48 h compared to the control group. Relative to the control group, BBB permeability was significantly reduced in the rutin-treated group. The results of the rotarod test revealed that rutin treatment significantly improved functional outcomes.
CONCLUSIONS: Rutin treatment starting 1h after injury attenuated BBB disruption during photothrombotic focal ischemia, which was partly, at least, achieved through inhibitory effects on MMP-9 expression and activity. The results of this study suggest that rutin might be useful in clinical trials aimed to improve the outcome of patients suffering from acute ischemic stroke.},
	language = {eng},
	number = {1-2},
	journal = {Journal of the Neurological Sciences},
	author = {Jang, Jae-Won and Lee, Jung-Kil and Hur, Hyuk and Kim, Tae-Wan and Joo, Sung-Pil and Piao, Min-Sheng},
	month = apr,
	year = {2014},
	pmid = {24507948},
	note = {00016 },
	keywords = {Animals, Blood-Brain Barrier, Brain Ischemia, Cerebral ischemia, Disease Models, Animal, Enzyme Inhibitors, Funcional outcome, Intracranial Thrombosis, Male, Matrix Metalloproteinase 9, Matrix metalloproteinases, Photic Stimulation, Photothrombosis, Rat, Rats, Rats, Sprague-Dawley, Recovery of Function, Rose Bengal, Rutin, Treatment Outcome},
	pages = {75--80}
}

{"_id":"3Cg8r4HdQboLPQeJx","bibbaseid":"jang-lee-hur-kim-joo-piao-rutinimprovesfunctionaloutcomeviareducingtheelevatedmatrixmetalloproteinase9levelinaphotothromboticfocalischemicmodelofrats-2014","downloads":0,"creationDate":"2016-12-16T08:43:40.772Z","title":"Rutin improves functional outcome via reducing the elevated matrix metalloproteinase-9 level in a photothrombotic focal ischemic model of rats","author_short":["Jang, J.","Lee, J.","Hur, H.","Kim, T.","Joo, S.","Piao, M."],"year":2014,"bibtype":"article","biburl":"http://bibbase.org/zotero/caplille","bibdata":{"bibtype":"article","type":"article","title":"Rutin improves functional outcome via reducing the elevated matrix metalloproteinase-9 level in a photothrombotic focal ischemic model of rats","volume":"339","issn":"1878-5883","doi":"10.1016/j.jns.2014.01.024","abstract":"BACKGROUND: Blood-brain barrier (BBB) disruption mediated by proteases plays a pivotal role in neural tissue damage after acute ischemic stroke. In an animal stroke model, the activation of matrix metalloproteinases (MMPs), especially MMP-9, was significantly increased and it showed potential association with blood-brain barrier (BBB) disruption and cerebral edema. Theoretically, it is expected that early blockade of expression and activation of MMP-9 after ischemic stroke provides neuroprotective effects from secondary neural tissue damage. This study was aimed to determine the ability of rutin to influence MMP-9 expression, activity and BBB disruption using a photothrombotic focal ischemic model in rats. METHODS: Adult male Sprague-Dawley rats, weighing between 250 and 300 g (aged 8 weeks) received focal cerebral ischemia by photothrombosis using Rose Bengal (RB) and cold light. Injured animals were divided into two groups; one group received 50mg/kg of rutin intraperitoneally, starting 1h after injury and at 12h intervals for 3 days, while animals in the control group received weight-adjusted doses of saline vehicle over the same period. In each group, the expressions and activities of MMP-9 were assessed by Western blot and gelatin zymography at 6, 24, 48, and 72 h after photothrombotic insult. The effects of rutin on BBB disruption and functional outcomes were also determined. RESULTS: Western blot and zymographic analysis showed up-regulated MMP-9 expression and activity in the ischemic cortex. The expression and activity of MMP-9 were significantly elevated at 6h after photothrombotic insult, which remained up-regulated for at least until 72 h after injury. In the rutin-treated group, MMP-9 expression and activity were significantly attenuated at 6, 24, and 48 h compared to the control group. Relative to the control group, BBB permeability was significantly reduced in the rutin-treated group. The results of the rotarod test revealed that rutin treatment significantly improved functional outcomes. CONCLUSIONS: Rutin treatment starting 1h after injury attenuated BBB disruption during photothrombotic focal ischemia, which was partly, at least, achieved through inhibitory effects on MMP-9 expression and activity. The results of this study suggest that rutin might be useful in clinical trials aimed to improve the outcome of patients suffering from acute ischemic stroke.","language":"eng","number":"1-2","journal":"Journal of the Neurological Sciences","author":[{"propositions":[],"lastnames":["Jang"],"firstnames":["Jae-Won"],"suffixes":[]},{"propositions":[],"lastnames":["Lee"],"firstnames":["Jung-Kil"],"suffixes":[]},{"propositions":[],"lastnames":["Hur"],"firstnames":["Hyuk"],"suffixes":[]},{"propositions":[],"lastnames":["Kim"],"firstnames":["Tae-Wan"],"suffixes":[]},{"propositions":[],"lastnames":["Joo"],"firstnames":["Sung-Pil"],"suffixes":[]},{"propositions":[],"lastnames":["Piao"],"firstnames":["Min-Sheng"],"suffixes":[]}],"month":"April","year":"2014","pmid":"24507948","note":"00016 ","keywords":"Animals, Blood-Brain Barrier, Brain Ischemia, Cerebral ischemia, Disease Models, Animal, Enzyme Inhibitors, Funcional outcome, Intracranial Thrombosis, Male, Matrix Metalloproteinase 9, Matrix metalloproteinases, Photic Stimulation, Photothrombosis, Rat, Rats, Rats, Sprague-Dawley, Recovery of Function, Rose Bengal, Rutin, Treatment Outcome","pages":"75--80","bibtex":"@article{jang_rutin_2014,\n\ttitle = {Rutin improves functional outcome via reducing the elevated matrix metalloproteinase-9 level in a photothrombotic focal ischemic model of rats},\n\tvolume = {339},\n\tissn = {1878-5883},\n\tdoi = {10.1016/j.jns.2014.01.024},\n\tabstract = {BACKGROUND: Blood-brain barrier (BBB) disruption mediated by proteases plays a pivotal role in neural tissue damage after acute ischemic stroke. In an animal stroke model, the activation of matrix metalloproteinases (MMPs), especially MMP-9, was significantly increased and it showed potential association with blood-brain barrier (BBB) disruption and cerebral edema. Theoretically, it is expected that early blockade of expression and activation of MMP-9 after ischemic stroke provides neuroprotective effects from secondary neural tissue damage. This study was aimed to determine the ability of rutin to influence MMP-9 expression, activity and BBB disruption using a photothrombotic focal ischemic model in rats.\nMETHODS: Adult male Sprague-Dawley rats, weighing between 250 and 300 g (aged 8 weeks) received focal cerebral ischemia by photothrombosis using Rose Bengal (RB) and cold light. Injured animals were divided into two groups; one group received 50mg/kg of rutin intraperitoneally, starting 1h after injury and at 12h intervals for 3 days, while animals in the control group received weight-adjusted doses of saline vehicle over the same period. In each group, the expressions and activities of MMP-9 were assessed by Western blot and gelatin zymography at 6, 24, 48, and 72 h after photothrombotic insult. The effects of rutin on BBB disruption and functional outcomes were also determined.\nRESULTS: Western blot and zymographic analysis showed up-regulated MMP-9 expression and activity in the ischemic cortex. The expression and activity of MMP-9 were significantly elevated at 6h after photothrombotic insult, which remained up-regulated for at least until 72 h after injury. In the rutin-treated group, MMP-9 expression and activity were significantly attenuated at 6, 24, and 48 h compared to the control group. Relative to the control group, BBB permeability was significantly reduced in the rutin-treated group. The results of the rotarod test revealed that rutin treatment significantly improved functional outcomes.\nCONCLUSIONS: Rutin treatment starting 1h after injury attenuated BBB disruption during photothrombotic focal ischemia, which was partly, at least, achieved through inhibitory effects on MMP-9 expression and activity. The results of this study suggest that rutin might be useful in clinical trials aimed to improve the outcome of patients suffering from acute ischemic stroke.},\n\tlanguage = {eng},\n\tnumber = {1-2},\n\tjournal = {Journal of the Neurological Sciences},\n\tauthor = {Jang, Jae-Won and Lee, Jung-Kil and Hur, Hyuk and Kim, Tae-Wan and Joo, Sung-Pil and Piao, Min-Sheng},\n\tmonth = apr,\n\tyear = {2014},\n\tpmid = {24507948},\n\tnote = {00016 },\n\tkeywords = {Animals, Blood-Brain Barrier, Brain Ischemia, Cerebral ischemia, Disease Models, Animal, Enzyme Inhibitors, Funcional outcome, Intracranial Thrombosis, Male, Matrix Metalloproteinase 9, Matrix metalloproteinases, Photic Stimulation, Photothrombosis, Rat, Rats, Rats, Sprague-Dawley, Recovery of Function, Rose Bengal, Rutin, Treatment Outcome},\n\tpages = {75--80}\n}\n\n","author_short":["Jang, J.","Lee, J.","Hur, H.","Kim, T.","Joo, S.","Piao, M."],"key":"jang_rutin_2014","id":"jang_rutin_2014","bibbaseid":"jang-lee-hur-kim-joo-piao-rutinimprovesfunctionaloutcomeviareducingtheelevatedmatrixmetalloproteinase9levelinaphotothromboticfocalischemicmodelofrats-2014","role":"author","urls":{},"keyword":["Animals","Blood-Brain Barrier","Brain Ischemia","Cerebral ischemia","Disease Models","Animal","Enzyme Inhibitors","Funcional outcome","Intracranial Thrombosis","Male","Matrix Metalloproteinase 9","Matrix metalloproteinases","Photic Stimulation","Photothrombosis","Rat","Rats","Rats","Sprague-Dawley","Recovery of Function","Rose Bengal","Rutin","Treatment Outcome"],"downloads":0,"html":""},"search_terms":["rutin","improves","functional","outcome","via","reducing","elevated","matrix","metalloproteinase","level","photothrombotic","focal","ischemic","model","rats","jang","lee","hur","kim","joo","piao"],"keywords":["animals","blood-brain barrier","brain ischemia","cerebral ischemia","disease models","animal","enzyme inhibitors","funcional outcome","intracranial thrombosis","male","matrix metalloproteinase 9","matrix metalloproteinases","photic stimulation","photothrombosis","rat","rats","rats","sprague-dawley","recovery of function","rose bengal","rutin","treatment outcome"],"authorIDs":[],"dataSources":["pPXo2k5Qsf43yyWNB"]}