Stem cell profiling by nuclear magnetic resonance spectroscopy. Jansen, J. F., Shamblott, M. J., van Zijl, P. C., Lehtimaki, K. K., Bulte, J. W., Gearhart, J. D., & Hakumaki, J. M. Magn Reson Med, 56(3):666-70, 2006. Jansen, Jacobus F A Shamblott, Michael J van Zijl, Peter C M Lehtimaki, Kimmo K Bulte, Jeff W M Gearhart, John D Hakumaki, Juhana M eng P41 EB015909/EB/NIBIB NIH HHS/ R01 NS045062/NS/NINDS NIH HHS/ RR11115/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2006/07/22 09:00 Magn Reson Med. 2006 Sep;56(3):666-70. doi: 10.1002/mrm.20968.
Paper doi abstract bibtex 2 downloads The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myo-inositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.
@article{RN126,
author = {Jansen, J. F. and Shamblott, M. J. and van Zijl, P. C. and Lehtimaki, K. K. and Bulte, J. W. and Gearhart, J. D. and Hakumaki, J. M.},
title = {Stem cell profiling by nuclear magnetic resonance spectroscopy},
journal = {Magn Reson Med},
volume = {56},
number = {3},
pages = {666-70},
note = {Jansen, Jacobus F A
Shamblott, Michael J
van Zijl, Peter C M
Lehtimaki, Kimmo K
Bulte, Jeff W M
Gearhart, John D
Hakumaki, Juhana M
eng
P41 EB015909/EB/NIBIB NIH HHS/
R01 NS045062/NS/NINDS NIH HHS/
RR11115/RR/NCRR NIH HHS/
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
2006/07/22 09:00
Magn Reson Med. 2006 Sep;56(3):666-70. doi: 10.1002/mrm.20968.},
abstract = {The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myo-inositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.},
keywords = {Animals
Cell Line
Magnetic Resonance Spectroscopy/*methods
Mice
Neurons/*cytology/*metabolism
Phosphorylcholine/*analysis
Stem Cells/*cytology/*metabolism},
ISSN = {0740-3194 (Print)
0740-3194 (Linking)},
DOI = {10.1002/mrm.20968},
url = {http://www.ncbi.nlm.nih.gov/pubmed/16858672
https://onlinelibrary.wiley.com/doi/full/10.1002/mrm.20968},
year = {2006},
type = {Journal Article}
}
Downloads: 2
{"_id":"B4EveW3sB7nY8txoH","bibbaseid":"jansen-shamblott-vanzijl-lehtimaki-bulte-gearhart-hakumaki-stemcellprofilingbynuclearmagneticresonancespectroscopy-2006","downloads":2,"creationDate":"2018-08-03T14:29:50.116Z","title":"Stem cell profiling by nuclear magnetic resonance spectroscopy","author_short":["Jansen, J. F.","Shamblott, M. J.","van Zijl, P. C.","Lehtimaki, K. K.","Bulte, J. W.","Gearhart, J. D.","Hakumaki, J. M."],"year":2006,"bibtype":"article","biburl":"https://raw.githubusercontent.com/jansenjfa1/bibbase.github.io/master/jansenjfa.bib","bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Jansen"],"firstnames":["J.","F."],"suffixes":[]},{"propositions":[],"lastnames":["Shamblott"],"firstnames":["M.","J."],"suffixes":[]},{"propositions":["van"],"lastnames":["Zijl"],"firstnames":["P.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Lehtimaki"],"firstnames":["K.","K."],"suffixes":[]},{"propositions":[],"lastnames":["Bulte"],"firstnames":["J.","W."],"suffixes":[]},{"propositions":[],"lastnames":["Gearhart"],"firstnames":["J.","D."],"suffixes":[]},{"propositions":[],"lastnames":["Hakumaki"],"firstnames":["J.","M."],"suffixes":[]}],"title":"Stem cell profiling by nuclear magnetic resonance spectroscopy","journal":"Magn Reson Med","volume":"56","number":"3","pages":"666-70","note":"Jansen, Jacobus F A Shamblott, Michael J van Zijl, Peter C M Lehtimaki, Kimmo K Bulte, Jeff W M Gearhart, John D Hakumaki, Juhana M eng P41 EB015909/EB/NIBIB NIH HHS/ R01 NS045062/NS/NINDS NIH HHS/ RR11115/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2006/07/22 09:00 Magn Reson Med. 2006 Sep;56(3):666-70. doi: 10.1002/mrm.20968.","abstract":"The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myo-inositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.","keywords":"Animals Cell Line Magnetic Resonance Spectroscopy/*methods Mice Neurons/*cytology/*metabolism Phosphorylcholine/*analysis Stem Cells/*cytology/*metabolism","issn":"0740-3194 (Print) 0740-3194 (Linking)","doi":"10.1002/mrm.20968","url":"http://www.ncbi.nlm.nih.gov/pubmed/16858672 https://onlinelibrary.wiley.com/doi/full/10.1002/mrm.20968","year":"2006","bibtex":"@article{RN126,\n author = {Jansen, J. F. and Shamblott, M. J. and van Zijl, P. C. and Lehtimaki, K. K. and Bulte, J. W. and Gearhart, J. D. and Hakumaki, J. M.},\n title = {Stem cell profiling by nuclear magnetic resonance spectroscopy},\n journal = {Magn Reson Med},\n volume = {56},\n number = {3},\n pages = {666-70},\n note = {Jansen, Jacobus F A\nShamblott, Michael J\nvan Zijl, Peter C M\nLehtimaki, Kimmo K\nBulte, Jeff W M\nGearhart, John D\nHakumaki, Juhana M\neng\nP41 EB015909/EB/NIBIB NIH HHS/\nR01 NS045062/NS/NINDS NIH HHS/\nRR11115/RR/NCRR NIH HHS/\nResearch Support, N.I.H., Extramural\nResearch Support, Non-U.S. Gov't\n2006/07/22 09:00\nMagn Reson Med. 2006 Sep;56(3):666-70. doi: 10.1002/mrm.20968.},\n abstract = {The classification of embryonic and adult stem cells, including their derivatives, is still limited, and often these cells are best defined by their functional properties. Recent gene array studies have yielded contradictory results. Also, very little is known about the metabolic properties of these exciting cells. In this study, proton (1H) NMR spectroscopy was used to identify the major low-molecular-weight metabolites in murine embryonic stem cells (ESC) and their neural stem cell (NSC) derivatives. ESC are characterized by an unusually low number of NMR-detectable metabolites, high phosphocholine (PC) content, and nondetectable glycerophosphocholine (GPC). The metabolic profiles of NSC resemble glial cells and oligodendrocyte progenitors, but with considerably higher PC, GPC, and myo-inositol content. The results suggest that NMR spectroscopy in vitro can provide markers to study the effects of differentiation on cell metabolism, and potentially to assess stem cell preparations for differentiation status.},\n keywords = {Animals\nCell Line\nMagnetic Resonance Spectroscopy/*methods\nMice\nNeurons/*cytology/*metabolism\nPhosphorylcholine/*analysis\nStem Cells/*cytology/*metabolism},\n ISSN = {0740-3194 (Print)\n0740-3194 (Linking)},\n DOI = {10.1002/mrm.20968},\n url = {http://www.ncbi.nlm.nih.gov/pubmed/16858672\nhttps://onlinelibrary.wiley.com/doi/full/10.1002/mrm.20968},\n year = {2006},\n type = {Journal Article}\n}\n\n","author_short":["Jansen, J. F.","Shamblott, M. J.","van Zijl, P. C.","Lehtimaki, K. K.","Bulte, J. W.","Gearhart, J. D.","Hakumaki, J. M."],"key":"RN126","id":"RN126","bibbaseid":"jansen-shamblott-vanzijl-lehtimaki-bulte-gearhart-hakumaki-stemcellprofilingbynuclearmagneticresonancespectroscopy-2006","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/16858672 https://onlinelibrary.wiley.com/doi/full/10.1002/mrm.20968"},"keyword":["Animals Cell Line Magnetic Resonance Spectroscopy/*methods Mice Neurons/*cytology/*metabolism Phosphorylcholine/*analysis Stem Cells/*cytology/*metabolism"],"metadata":{"authorlinks":{"jansen, j":"https://www.jansenjfa.com/publications/"}},"downloads":2,"html":""},"search_terms":["stem","cell","profiling","nuclear","magnetic","resonance","spectroscopy","jansen","shamblott","van zijl","lehtimaki","bulte","gearhart","hakumaki"],"keywords":["animals cell line magnetic resonance spectroscopy/*methods mice neurons/*cytology/*metabolism phosphorylcholine/*analysis stem cells/*cytology/*metabolism"],"authorIDs":["TEAWLnKpXtQE8hs6C"],"dataSources":["TCkfRWJAZvbLAZi29"]}