@ARTICLE{Janssen2015-wk,
  title    = "Antibody activation using {DNA-based} logic gates",
  author   = "Janssen, Brian M G and van Rosmalen, Martijn and van Beek, Lotte
              and Merkx, Maarten",
  abstract = "Oligonucleotide-based molecular circuits offer the exciting
              possibility to introduce autonomous signal processing in
              biomedicine, synthetic biology, and molecular diagnostics. Here
              we introduce bivalent peptide-DNA conjugates as generic,
              noncovalent, and easily applicable molecular locks that allow the
              control of antibody activity using toehold-mediated strand
              displacement reactions. Employing yeast as a cellular model
              system, reversible control of antibody targeting is demonstrated
              with low nM concentrations of peptide-DNA locks and
              oligonucleotide displacer strands. Introduction of two different
              toehold strands on the peptide-DNA lock allowed signal
              integration of two different inputs, yielding logic OR- and
              AND-gates. The range of molecular inputs could be further
              extended to protein-based triggers by using protein-binding
              aptamers.",
  journal  = "Angew Chem Int Ed Engl",
  volume   =  54,
  number   =  8,
  pages    = "2530--2533",
  month    =  jan,
  year     =  2015,
  address  = "Germany",
  keywords = "DNA nanotechnology; antibodies; aptamers; molecular computing;
              peptide-oligonucleotide conjugates",
  language = "en"
}

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