Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography–Mass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) Evaluated against Alternative Methods. Janssens, G., Mangelinckx, S., Courtheyn, D., De Kimpe, N., Matthijs, B., & Le Bizec, B. 63(34):7574–7581.
Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography–Mass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) Evaluated against Alternative Methods [link]Paper  doi  abstract   bibtex   
The administration of synthetic homologues of naturally occurring steroids can be demonstrated by measuring 13C/12C isotopic ratios of their urinary metabolites. Gas chromatography−mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) was used in this study to appraise in a global approach isotopic deviations of two 17βtestosterone metabolites (17α-testosterone and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol) together with those of 5-androstene-3β,17α-diol as endogenous reference compound (ERC). Intermediate precisions of 0.35‰, 1.05‰, 0.35‰, and 0.21‰, respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17β-testosterone propionate and 17β-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17β-estradiol treatment up to 24 days. For 17β-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. The capability of GC-MS/C/IRMS to demonstrate natural steroid abuse for urinary steroids was eventually compared to those of mass spectrometry (LC-MS/MS) when measuring intact steroid esters in blood and hair.
@article{janssens_simultaneous_2015,
	title = {Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography–Mass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry ({GC}-{MS}/C/{IRMS}) Evaluated against Alternative Methods},
	volume = {63},
	issn = {0021-8561, 1520-5118},
	url = {http://pubs.acs.org/doi/10.1021/acs.jafc.5b02746},
	doi = {10.1021/acs.jafc.5b02746},
	abstract = {The administration of synthetic homologues of naturally occurring steroids can be demonstrated by measuring 13C/12C isotopic ratios of their urinary metabolites. Gas chromatography−mass spectrometry/combustion/isotope ratio mass spectrometry ({GC}-{MS}/C/{IRMS}) was used in this study to appraise in a global approach isotopic deviations of two 17βtestosterone metabolites (17α-testosterone and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol) together with those of 5-androstene-3β,17α-diol as endogenous reference compound ({ERC}). Intermediate precisions of 0.35‰, 1.05‰, 0.35‰, and 0.21‰, respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17β-testosterone propionate and 17β-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17β-estradiol treatment up to 24 days. For 17β-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. The capability of {GC}-{MS}/C/{IRMS} to demonstrate natural steroid abuse for urinary steroids was eventually compared to those of mass spectrometry ({LC}-{MS}/{MS}) when measuring intact steroid esters in blood and hair.},
	pages = {7574--7581},
	number = {34},
	journaltitle = {Journal of Agricultural and Food Chemistry},
	author = {Janssens, Geert and Mangelinckx, Sven and Courtheyn, Dirk and De Kimpe, Norbert and Matthijs, Bert and Le Bizec, Bruno},
	urldate = {2019-03-29},
	date = {2015-09-02},
	langid = {english},
	file = {Janssens et al. - 2015 - Simultaneous Detection of Androgen and Estrogen Ab.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\B82XNF86\\Janssens et al. - 2015 - Simultaneous Detection of Androgen and Estrogen Ab.pdf:application/pdf}
}
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