Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography–Mass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) Evaluated against Alternative Methods. Janssens, G., Mangelinckx, S., Courtheyn, D., De Kimpe, N., Matthijs, B., & Le Bizec, B. 63(34):7574–7581. Paper doi abstract bibtex The administration of synthetic homologues of naturally occurring steroids can be demonstrated by measuring 13C/12C isotopic ratios of their urinary metabolites. Gas chromatography−mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) was used in this study to appraise in a global approach isotopic deviations of two 17βtestosterone metabolites (17α-testosterone and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol) together with those of 5-androstene-3β,17α-diol as endogenous reference compound (ERC). Intermediate precisions of 0.35‰, 1.05‰, 0.35‰, and 0.21‰, respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17β-testosterone propionate and 17β-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17β-estradiol treatment up to 24 days. For 17β-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. The capability of GC-MS/C/IRMS to demonstrate natural steroid abuse for urinary steroids was eventually compared to those of mass spectrometry (LC-MS/MS) when measuring intact steroid esters in blood and hair.
@article{janssens_simultaneous_2015,
title = {Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography–Mass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry ({GC}-{MS}/C/{IRMS}) Evaluated against Alternative Methods},
volume = {63},
issn = {0021-8561, 1520-5118},
url = {http://pubs.acs.org/doi/10.1021/acs.jafc.5b02746},
doi = {10.1021/acs.jafc.5b02746},
abstract = {The administration of synthetic homologues of naturally occurring steroids can be demonstrated by measuring 13C/12C isotopic ratios of their urinary metabolites. Gas chromatography−mass spectrometry/combustion/isotope ratio mass spectrometry ({GC}-{MS}/C/{IRMS}) was used in this study to appraise in a global approach isotopic deviations of two 17βtestosterone metabolites (17α-testosterone and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol) together with those of 5-androstene-3β,17α-diol as endogenous reference compound ({ERC}). Intermediate precisions of 0.35‰, 1.05‰, 0.35‰, and 0.21‰, respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17β-testosterone propionate and 17β-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17β-estradiol treatment up to 24 days. For 17β-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. The capability of {GC}-{MS}/C/{IRMS} to demonstrate natural steroid abuse for urinary steroids was eventually compared to those of mass spectrometry ({LC}-{MS}/{MS}) when measuring intact steroid esters in blood and hair.},
pages = {7574--7581},
number = {34},
journaltitle = {Journal of Agricultural and Food Chemistry},
author = {Janssens, Geert and Mangelinckx, Sven and Courtheyn, Dirk and De Kimpe, Norbert and Matthijs, Bert and Le Bizec, Bruno},
urldate = {2019-03-29},
date = {2015-09-02},
langid = {english},
file = {Janssens et al. - 2015 - Simultaneous Detection of Androgen and Estrogen Ab.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\B82XNF86\\Janssens et al. - 2015 - Simultaneous Detection of Androgen and Estrogen Ab.pdf:application/pdf}
}
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Gas chromatography−mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) was used in this study to appraise in a global approach isotopic deviations of two 17βtestosterone metabolites (17α-testosterone and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol) together with those of 5-androstene-3β,17α-diol as endogenous reference compound (ERC). Intermediate precisions of 0.35‰, 1.05‰, 0.35‰, and 0.21‰, respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17β-testosterone propionate and 17β-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17β-estradiol treatment up to 24 days. For 17β-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. The capability of GC-MS/C/IRMS to demonstrate natural steroid abuse for urinary steroids was eventually compared to those of mass spectrometry (LC-MS/MS) when measuring intact steroid esters in blood and hair.","pages":"7574–7581","number":"34","journaltitle":"Journal of Agricultural and Food Chemistry","author":[{"propositions":[],"lastnames":["Janssens"],"firstnames":["Geert"],"suffixes":[]},{"propositions":[],"lastnames":["Mangelinckx"],"firstnames":["Sven"],"suffixes":[]},{"propositions":[],"lastnames":["Courtheyn"],"firstnames":["Dirk"],"suffixes":[]},{"propositions":[],"lastnames":["De","Kimpe"],"firstnames":["Norbert"],"suffixes":[]},{"propositions":[],"lastnames":["Matthijs"],"firstnames":["Bert"],"suffixes":[]},{"propositions":[],"lastnames":["Le","Bizec"],"firstnames":["Bruno"],"suffixes":[]}],"urldate":"2019-03-29","date":"2015-09-02","langid":"english","file":"Janssens et al. - 2015 - Simultaneous Detection of Androgen and Estrogen Ab.pdf:C\\:\\\\Users\\\\ygu\\\\Documents\\¶CPOR066_YGU\\\\YGU\\\\Zotero\\\\storage\\\\B82XNF86\\\\Janssens et al. - 2015 - Simultaneous Detection of Androgen and Estrogen Ab.pdf:application/pdf","bibtex":"@article{janssens_simultaneous_2015,\n\ttitle = {Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography–Mass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry ({GC}-{MS}/C/{IRMS}) Evaluated against Alternative Methods},\n\tvolume = {63},\n\tissn = {0021-8561, 1520-5118},\n\turl = {http://pubs.acs.org/doi/10.1021/acs.jafc.5b02746},\n\tdoi = {10.1021/acs.jafc.5b02746},\n\tabstract = {The administration of synthetic homologues of naturally occurring steroids can be demonstrated by measuring 13C/12C isotopic ratios of their urinary metabolites. Gas chromatography−mass spectrometry/combustion/isotope ratio mass spectrometry ({GC}-{MS}/C/{IRMS}) was used in this study to appraise in a global approach isotopic deviations of two 17βtestosterone metabolites (17α-testosterone and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol) together with those of 5-androstene-3β,17α-diol as endogenous reference compound ({ERC}). Intermediate precisions of 0.35‰, 1.05‰, 0.35‰, and 0.21‰, respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17β-testosterone propionate and 17β-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17β-estradiol treatment up to 24 days. For 17β-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. 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