The use of gas chromatography–mass spectrometry/combustion/isotope ratio mass spectrometry to demonstrate progesterone treatment in bovines. Janssens, G., Mangelinckx, S., Courtheyn, D., De Kimpe, N., Matthijs, B., & Le Bizec, B. 1449:129–140.
The use of gas chromatography–mass spectrometry/combustion/isotope ratio mass spectrometry to demonstrate progesterone treatment in bovines [link]Paper  doi  abstract   bibtex   
Currently, no analytical method is available to demonstrate progesterone administration in biological samples collected in rearing animals, and therefore, tracking the abuse of this popular growth promoter is arduous. In this study, a method is presented to reveal progesterone (PG) treatment on the basis of carbon isotope measurement of 5␤-pregnane-3␣, 20␣-diol (BAA-PD), a major PG metabolite excreted in bovine urine, by gas chromatography–mass spectrometry/combustion/isotope ratio mass spectrometry (GC–MS/C/IRMS). 5-Androstene-3␤,17␣-diol (AEdiol) is used as endogenous reference compound. Intermediate precisions (n = 11) of 0.56‰ and 0.68‰ have been determined for AEdiol and BAA-PD, respectively. The analytical method was used for the very first time to successfully differentiate urine samples collected in treated and untreated animals.
@article{janssens_use_2016,
	title = {The use of gas chromatography–mass spectrometry/combustion/isotope ratio mass spectrometry to demonstrate progesterone treatment in bovines},
	volume = {1449},
	issn = {00219673},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0021967316305295},
	doi = {10.1016/j.chroma.2016.04.074},
	abstract = {Currently, no analytical method is available to demonstrate progesterone administration in biological samples collected in rearing animals, and therefore, tracking the abuse of this popular growth promoter is arduous. In this study, a method is presented to reveal progesterone ({PG}) treatment on the basis of carbon isotope measurement of 5␤-pregnane-3␣, 20␣-diol ({BAA}-{PD}), a major {PG} metabolite excreted in bovine urine, by gas chromatography–mass spectrometry/combustion/isotope ratio mass spectrometry ({GC}–{MS}/C/{IRMS}). 5-Androstene-3␤,17␣-diol ({AEdiol}) is used as endogenous reference compound. Intermediate precisions (n = 11) of 0.56‰ and 0.68‰ have been determined for {AEdiol} and {BAA}-{PD}, respectively. The analytical method was used for the very first time to successfully differentiate urine samples collected in treated and untreated animals.},
	pages = {129--140},
	journaltitle = {Journal of Chromatography A},
	author = {Janssens, Geert and Mangelinckx, Sven and Courtheyn, Dirk and De Kimpe, Norbert and Matthijs, Bert and Le Bizec, Bruno},
	urldate = {2019-03-29},
	date = {2016-06},
	langid = {english},
	file = {Janssens et al. - 2016 - The use of gas chromatography–mass spectrometryco.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\ILXZLXE7\\Janssens et al. - 2016 - The use of gas chromatography–mass spectrometryco.pdf:application/pdf}
}
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