Frequency, kinetics and determinants of viable SARS-CoV-2 in bioaerosols from ambulatory COVID-19 patients infected with the Beta, Delta or Omicron variants. Jaumdally, S, Tomasicchio, M, Pooran, A, Esmail, A, Kotze, A, Meier, S, Wilson, L, Oelofse, S, van der Merwe, C, Roomaney, A, Davids, M, Suliman, T, Joseph, R, Perumal, T, Scott, A, Shaw, M, Preiser, W, Williamson, C, Goga, A, Mayne, E, Gray, G, Moore, P, Sigal, A, Limberis, J, Metcalfe, J, & Dheda, K Nature Communications, 15(1):2003, Nature Publishing Group, mar, 2024. ![Frequency, kinetics and determinants of viable SARS-CoV-2 in bioaerosols from ambulatory COVID-19 patients infected with the Beta, Delta or Omicron variants [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Airborne transmission of SARS-CoV-2 aerosol remains contentious. Importantly, whether cough or breath-generated bioaerosols can harbor viable and replicating virus remains largely unclarified. We performed size-fractionated aerosol sampling (Andersen cascade impactor) and evaluated viral culturability in human cell lines (infectiousness), viral genetics, and host immunity in ambulatory participants with COVID-19. Sixty-one percent (27/44) and 50% (22/44) of participants emitted variant-specific culture-positive aerosols \textless10$μ$m and \textless5$μ$m, respectively, for up to 9 days after symptom onset. Aerosol culturability is significantly associated with lower neutralizing antibody titers, and suppression of transcriptomic pathways related to innate immunity and the humoral response. A nasopharyngeal Ct \textless17 rules-in \ 40% of aerosol culture-positives and identifies those who are probably highly infectious. A parsimonious three transcript blood-based biosignature is highly predictive of infectious aerosol generation (PPV \textgreater 95%). There is considerable heterogeneity in potential infectiousness i.e., only 29% of participants were probably highly infectious (produced culture-positive aerosols \textless5$μ$m at \ 6 days after symptom onset). These data, which comprehensively confirm variant-specific culturable SARS-CoV-2 in aerosol, inform the targeting of transmission-related interventions and public health containment strategies emphasizing improved ventilation. SARS-CoV-2 can be spread by aerosols. Here the authors show that between 50-60% of ambulatory COVID-19 patients exhale culturable virus and that this is associated with lower neutralizing antibody titers and suppression of immune related transcriptomic pathways.
@article{Jaumdally2024,
abstract = {Airborne transmission of SARS-CoV-2 aerosol remains contentious. Importantly, whether cough or breath-generated bioaerosols can harbor viable and replicating virus remains largely unclarified. We performed size-fractionated aerosol sampling (Andersen cascade impactor) and evaluated viral culturability in human cell lines (infectiousness), viral genetics, and host immunity in ambulatory participants with COVID-19. Sixty-one percent (27/44) and 50{\%} (22/44) of participants emitted variant-specific culture-positive aerosols {\textless}10$\mu$m and {\textless}5$\mu$m, respectively, for up to 9 days after symptom onset. Aerosol culturability is significantly associated with lower neutralizing antibody titers, and suppression of transcriptomic pathways related to innate immunity and the humoral response. A nasopharyngeal Ct {\textless}17 rules-in {\~{}}40{\%} of aerosol culture-positives and identifies those who are probably highly infectious. A parsimonious three transcript blood-based biosignature is highly predictive of infectious aerosol generation (PPV {\textgreater} 95{\%}). There is considerable heterogeneity in potential infectiousness i.e., only 29{\%} of participants were probably highly infectious (produced culture-positive aerosols {\textless}5$\mu$m at {\~{}}6 days after symptom onset). These data, which comprehensively confirm variant-specific culturable SARS-CoV-2 in aerosol, inform the targeting of transmission-related interventions and public health containment strategies emphasizing improved ventilation. SARS-CoV-2 can be spread by aerosols. Here the authors show that between 50-60{\%} of ambulatory COVID-19 patients exhale culturable virus and that this is associated with lower neutralizing antibody titers and suppression of immune related transcriptomic pathways.},
author = {Jaumdally, S and Tomasicchio, M and Pooran, A and Esmail, A and Kotze, A and Meier, S and Wilson, L and Oelofse, S and van der Merwe, C and Roomaney, A and Davids, M and Suliman, T and Joseph, R and Perumal, T and Scott, A and Shaw, M and Preiser, W and Williamson, C and Goga, A and Mayne, E and Gray, G and Moore, P and Sigal, A and Limberis, J and Metcalfe, J and Dheda, K},
doi = {10.1038/s41467-024-45400-1},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Jaumdally et al. - 2024 - Frequency, kinetics and determinants of viable SARS-CoV-2 in bioaerosols from ambulatory COVID-19 patients inf.pdf:pdf},
issn = {2041-1723},
journal = {Nature Communications},
keywords = {2,CoV,Diagnostic markers,Molecular medicine,OA,Respiratory tract diseases,SARS,fund{\_}not{\_}ack,original},
mendeley-tags = {OA,fund{\_}not{\_}ack,original},
month = {mar},
number = {1},
pages = {2003},
publisher = {Nature Publishing Group},
title = {{Frequency, kinetics and determinants of viable SARS-CoV-2 in bioaerosols from ambulatory COVID-19 patients infected with the Beta, Delta or Omicron variants}},
url = {https://www.nature.com/articles/s41467-024-45400-1},
volume = {15},
year = {2024}
}
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We performed size-fractionated aerosol sampling (Andersen cascade impactor) and evaluated viral culturability in human cell lines (infectiousness), viral genetics, and host immunity in ambulatory participants with COVID-19. Sixty-one percent (27/44) and 50% (22/44) of participants emitted variant-specific culture-positive aerosols \\textless10$μ$m and \\textless5$μ$m, respectively, for up to 9 days after symptom onset. Aerosol culturability is significantly associated with lower neutralizing antibody titers, and suppression of transcriptomic pathways related to innate immunity and the humoral response. A nasopharyngeal Ct \\textless17 rules-in \\ 40% of aerosol culture-positives and identifies those who are probably highly infectious. A parsimonious three transcript blood-based biosignature is highly predictive of infectious aerosol generation (PPV \\textgreater 95%). There is considerable heterogeneity in potential infectiousness i.e., only 29% of participants were probably highly infectious (produced culture-positive aerosols \\textless5$μ$m at \\ 6 days after symptom onset). These data, which comprehensively confirm variant-specific culturable SARS-CoV-2 in aerosol, inform the targeting of transmission-related interventions and public health containment strategies emphasizing improved ventilation. SARS-CoV-2 can be spread by aerosols. 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