Role of P‐450 aromatase in sex determination of the diamondback terrapin, Malaclemys terrapin

Role of P‐450 aromatase in sex determination of the diamondback terrapin, Malaclemys terrapin. Jeyasuria, P., Roosenburg, W. M., & Place, A. R. Journal of Experimental Zoology, 270(1):95–111, September, 1994.

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Sex determination in the diamondback terrapin, Malaclemys terrapin, is temperature-dependent. Eggs incubated a t 31"C, and above, hatch in approximately 45 days as females. Eggs incubated below 27°C hatch in about 60 days as males. Sex is not reversible after hatching. Nest temperatures in the wild can be as low as 20°C and as high as 37°C with as much as a 10°C die1 cycle. The shortest incubation time measured in nature was 56 days and the longest approaching 120 days. Nests in our study site produced predominantly (\textgreater 95%) male hatchlings. Treatment of developing embryos with estrogen produces females at male producing temperatures while treatment with fadrozole (a nonsteroidal aromatase inhibitor) induces partial male-like gonads. Treatment with a steroidal aromatase inhibitor (4-hydroxyandrostenedione, 4-OHA) had no effect on sex determination. Both fadrozole and 4-OHA are potent competitive inhibitors (K, approximately 40-50 nM) for terrapin in vitro arornatase activity. These findings are consistent with aromatase expression being a key step in sex determination of terrapins. We have cloned a partial single copy P-450 aromatase from the terrapin using a cDNA library constructed from ovarian mRNA. This partial clone is highly homologous t o other vertebrate aromatases. 01994 Wiley-Liss, Inc.

@article{jeyasuria_role_1994,
	title = {Role of {P}‐450 aromatase in sex determination of the diamondback terrapin, \textit{{Malaclemys} terrapin}},
	volume = {270},
	copyright = {http://onlinelibrary.wiley.com/termsAndConditions\#vor},
	issn = {0022-104X, 1097-010X},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/jez.1402700111},
	doi = {10.1002/jez.1402700111},
	abstract = {Sex determination in the diamondback terrapin, Malaclemys terrapin, is temperature-dependent. Eggs incubated a t 31"C, and above, hatch in approximately 45 days as females. Eggs incubated below 27°C hatch in about 60 days as males. Sex is not reversible after hatching. Nest temperatures in the wild can be as low as 20°C and as high as 37°C with as much as a 10°C die1 cycle. The shortest incubation time measured in nature was 56 days and the longest approaching 120 days. Nests in our study site produced predominantly ({\textgreater} 95\%) male hatchlings. Treatment of developing embryos with estrogen produces females at male producing temperatures while treatment with fadrozole (a nonsteroidal aromatase inhibitor) induces partial male-like gonads. Treatment with a steroidal aromatase inhibitor (4-hydroxyandrostenedione, 4-OHA) had no effect on sex determination. Both fadrozole and 4-OHA are potent competitive inhibitors (K, approximately 40-50 nM) for terrapin in vitro arornatase activity. These findings are consistent with aromatase expression being a key step in sex determination of terrapins. We have cloned a partial single copy P-450 aromatase from the terrapin using a cDNA library constructed from ovarian mRNA. This partial clone is highly homologous t o other vertebrate aromatases. 01994 Wiley-Liss, Inc.},
	language = {en},
	number = {1},
	urldate = {2025-05-23},
	journal = {Journal of Experimental Zoology},
	author = {Jeyasuria, Pancharatnam and Roosenburg, Willem M. and Place, Allen R.},
	month = sep,
	year = {1994},
	pages = {95--111},
}

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