Simulated Microgravity and 3D Culture Enhance Induction, Viability, Proliferation and Differentiation of Cardiac Progenitors from Human Pluripotent Stem Cells. Jha, R, Wu, Q, Singh, M, Preininger, M., Han, P, Ding, G, Cho, H., Jo, H, Maher, K., Wagner, M., & Xu, C Sci Rep, 6:30956.
Simulated Microgravity and 3D Culture Enhance Induction, Viability, Proliferation and Differentiation of Cardiac Progenitors from Human Pluripotent Stem Cells. [link]Paper  doi  abstract   bibtex   
Efficient generation of cardiomyocytes from human pluripotent stem cells is critical for their regenerative applications. Microgravity and 3D culture can profoundly modulate cell proliferation and survival. Here, we engineered microscale progenitor cardiac spheres from human pluripotent stem cells and exposed the spheres to simulated microgravity using a random positioning machine for 3 days during their differentiation to cardiomyocytes. This process resulted in the production of highly enriched cardiomyocytes (99% purity) with high viability (90%) and expected functional properties, with a 1.5 to 4-fold higher yield of cardiomyocytes from each undifferentiated stem cell as compared with 3D-standard gravity culture. Increased induction, proliferation and viability of cardiac progenitors as well as up-regulation of genes associated with proliferation and survival at the early stage of differentiation were observed in the 3D culture under simulated microgravity. Therefore, a combination of 3D culture and simulated microgravity can be used to efficiently generate highly enriched cardiomyocytes.
@article{jha_simulated_nodate,
	title = {Simulated {Microgravity} and 3D {Culture} {Enhance} {Induction}, {Viability}, {Proliferation} and {Differentiation} of {Cardiac} {Progenitors} from {Human} {Pluripotent} {Stem} {Cells}.},
	volume = {6},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/27492371},
	doi = {10.1038/srep30956},
	abstract = {Efficient generation of cardiomyocytes from human pluripotent stem cells is critical for their regenerative applications. Microgravity and 3D culture can profoundly modulate cell proliferation and survival. Here, we engineered microscale progenitor cardiac spheres from human pluripotent stem cells and exposed the spheres to simulated microgravity using a random positioning machine for 3 days during their differentiation to cardiomyocytes. This process resulted in the production of highly enriched cardiomyocytes (99\% purity) with high viability (90\%) and expected functional properties, with a 1.5 to 4-fold higher yield of cardiomyocytes from each undifferentiated stem cell as compared with 3D-standard gravity culture. Increased induction, proliferation and viability of cardiac progenitors as well as up-regulation of genes associated with proliferation and survival at the early stage of differentiation were observed in the 3D culture under simulated microgravity. Therefore, a combination of 3D culture and simulated microgravity can be used to efficiently generate highly enriched cardiomyocytes.},
	language = {eng},
	journal = {Sci Rep},
	author = {Jha, R and Wu, Q and Singh, M and Preininger, MK and Han, P and Ding, G and Cho, HC and Jo, H and Maher, KO and Wagner, MB and Xu, C},
	keywords = {Weightlessness Simulation},
	pages = {30956}
}
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