@article{jha_efficient_2015,
	title = {Efficient differentiation of cardiomyocytes from human pluripotent stem cells with growth factors.},
	volume = {1299},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/25836579},
	doi = {10.1007/978-1-4939-2572-8_9},
	abstract = {Human pluripotent stem cells have tremendous replicative capacity and demonstrated potential to generate functional cardiomyocytes. These cardiomyocytes represent a promising source for cell replacement therapy to treat heart disease and may serve as a useful tool for drug discovery and disease modeling. Efficient cardiomyocyte differentiation, a prerequisite for the application of stem cell-derived cardiomyocytes, can be achieved with a growth factor-guided method. Undifferentiated cells are sequentially treated with activin A and BMP4 in a serum-free and insulin-free medium and then maintained in a serum-free medium with insulin. This method yields as much as {\textgreater}75\% cardiomyocytes in the differentiation culture within 2 weeks, and the beating cardiomyocytes have expected molecular, cellular, and electrophysiological characteristics. In this chapter, we describe in detail the differentiation protocol and follow-up characterization focusing on immunocytochemistry, quantitative RT-PCR, and flow cytometry analysis.},
	language = {eng},
	journal = {Methods Mol Biol},
	author = {Jha, R and Xu, R-H and Xu, C},
	year = {2015},
	keywords = {Real-Time Polymerase Chain Reaction},
	pages = {115--131}
}

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