Selective targeting of parallel G-quadruplex structure using L-RNA aptamer. Ji, D., Yuan, J., Chen, S., Tan, J., & Kwok, C. Nucleic Acids Research, October, 2023. ![Selective targeting of parallel G-quadruplex structure using L-RNA aptamer [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Abstract G-quadruplexes (G4) are special nucleic acid structures with diverse conformational polymorphisms. Selective targeting of G-quadruplex conformations and regulating their biological functions provide promising therapeutic intervention. Despite the large repertoire of G4-binding tools, only a limited number of them can specifically target a particular G4 conformation. Here, we introduce a novel method, G4-SELEX-Seq and report the development of the first L-RNA aptamer, L-Apt12-6, with high binding selectivity to parallel G4 over other nucleic acid structures. Using parallel dG4 c-kit 1 as an example, we demonstrate the strong binding affinity between L-Apt12-6 and c-kit 1 dG4 in vitro and in cells, and notably report the applications of L-Apt12-6 in controlling DNA replication and gene expression. Our results suggest that L-Apt12-6 is a valuable tool for targeting parallel G-quadruplex conformation and regulating G4-mediated biological processes. Furthermore, G4-SELEX-Seq can be used as a general platform for G4-targeting L-RNA aptamers selection and should be applicable to other nucleic acid structures.
@article{ji_selective_2023,
title = {Selective targeting of parallel {G}-quadruplex structure using {L}-{RNA} aptamer},
issn = {0305-1048, 1362-4962},
url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad900/7327056},
doi = {10.1093/nar/gkad900},
abstract = {Abstract
G-quadruplexes (G4) are special nucleic acid structures with diverse conformational polymorphisms. Selective targeting of G-quadruplex conformations and regulating their biological functions provide promising therapeutic intervention. Despite the large repertoire of G4-binding tools, only a limited number of them can specifically target a particular G4 conformation. Here, we introduce a novel method, G4-SELEX-Seq and report the development of the first L-RNA aptamer, L-Apt12-6, with high binding selectivity to parallel G4 over other nucleic acid structures. Using parallel dG4 c-kit 1 as an example, we demonstrate the strong binding affinity between L-Apt12-6 and c-kit 1 dG4 in vitro and in cells, and notably report the applications of L-Apt12-6 in controlling DNA replication and gene expression. Our results suggest that L-Apt12-6 is a valuable tool for targeting parallel G-quadruplex conformation and regulating G4-mediated biological processes. Furthermore, G4-SELEX-Seq can be used as a general platform for G4-targeting L-RNA aptamers selection and should be applicable to other nucleic acid structures.},
language = {en},
urldate = {2023-11-03},
journal = {Nucleic Acids Research},
author = {Ji, Danyang and Yuan, Jia-Hao and Chen, Shuo-Bin and Tan, Jia-Heng and Kwok, Chun Kit},
month = oct,
year = {2023},
pages = {gkad900},
}
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