Development of 23 novel polymorphic EST-SSR markers for the endangered relict conifer Metasequoia glyptostroboides. Jin, Y., Bi, Q., Guan, W., & Mao, J. Applications in Plant Sciences, 3(9):1500038, 2015. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.3732/apps.1500038![Development of 23 novel polymorphic EST-SSR markers for the endangered relict conifer Metasequoia glyptostroboides [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Premise of the study: Metasequoia glyptostroboides is an endangered relict conifer species endemic to China. In this study, expressed sequence tag–simple sequence repeat (EST-SSR) markers were developed using transcriptome mining for future genetic and functional studies. Methods and Results: We collected 97,565 unigene sequences generated by 454 pyrosequencing. A bioinformatics analysis identified 2087 unique and putative microsatellites, from which 96 novel microsatellite markers were developed. Fifty-three of the 96 primer sets successfully amplified clear fragments of the expected sizes; 23 of those loci were polymorphic. The number of alleles per locus ranged from two to eight, with an average of three, and the observed and expected heterozygosity values ranged from 0 to 1.0 and 0.117 to 0.813, respectively. Conclusions: These microsatellite loci will enrich the genetic resources to develop functional studies and conservation strategies for this endangered relict species.
@article{jin_development_2015,
title = {Development of 23 novel polymorphic {EST}-{SSR} markers for the endangered relict conifer {Metasequoia} glyptostroboides},
volume = {3},
issn = {2168-0450},
url = {https://onlinelibrary.wiley.com/doi/abs/10.3732/apps.1500038},
doi = {10.3732/apps.1500038},
abstract = {Premise of the study: Metasequoia glyptostroboides is an endangered relict conifer species endemic to China. In this study, expressed sequence tag–simple sequence repeat (EST-SSR) markers were developed using transcriptome mining for future genetic and functional studies. Methods and Results: We collected 97,565 unigene sequences generated by 454 pyrosequencing. A bioinformatics analysis identified 2087 unique and putative microsatellites, from which 96 novel microsatellite markers were developed. Fifty-three of the 96 primer sets successfully amplified clear fragments of the expected sizes; 23 of those loci were polymorphic. The number of alleles per locus ranged from two to eight, with an average of three, and the observed and expected heterozygosity values ranged from 0 to 1.0 and 0.117 to 0.813, respectively. Conclusions: These microsatellite loci will enrich the genetic resources to develop functional studies and conservation strategies for this endangered relict species.},
language = {en},
number = {9},
urldate = {2023-04-27},
journal = {Applications in Plant Sciences},
author = {Jin, Yuqing and Bi, Quanxin and Guan, Wenbin and Mao, Jian-Feng},
year = {2015},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.3732/apps.1500038},
keywords = {454 pyrosequencing, Cupressaceae, EST-SSR, Metasequoia glyptostroboides, microsatellite, relict plant},
pages = {1500038},
}
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