Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate. Jin, M., Bothfeld, W., Austin, S., Sato, T., K., La Reau, A., Li, H., Foston, M., Gunawan, C., LeDuc, R., D., Quensen, J., F., Mcgee, M., Uppugundla, N., Higbee, A., Ranatunga, R., Donald, C., W., Bone, G., Ragauskas, A., J., Tiedje, J., M., Noguera, D., R., Dale, B., E., Zhang, Y., & Balan, V. Bioresource Technology, 147:212-220, 2013.
Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate [link]Website  abstract   bibtex   
To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate. © 2013 Elsevier Ltd.
@article{
 title = {Effect of storage conditions on the stability and fermentability of enzymatic lignocellulosic hydrolysate},
 type = {article},
 year = {2013},
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 keywords = {PY2},
 pages = {212-220},
 volume = {147},
 websites = {http://www.sciencedirect.com/science/article/pii/S0960852413012479},
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 abstract = {To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX™ (Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4°C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate. © 2013 Elsevier Ltd.},
 bibtype = {article},
 author = {Jin, Mingjie and Bothfeld, William and Austin, Samantha and Sato, Trey K. and La Reau, Alex and Li, Haibo and Foston, Marcus and Gunawan, Christa and LeDuc, Richard D. and Quensen, John F. and Mcgee, Mick and Uppugundla, Nirmal and Higbee, Alan and Ranatunga, Ruwan and Donald, Charles W. and Bone, Gwen and Ragauskas, Arthur J. and Tiedje, James M. and Noguera, Daniel R. and Dale, Bruce E. and Zhang, Yaoping and Balan, Venkatesh},
 journal = {Bioresource Technology}
}

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