How to build a time-gated luminescence microscope. Jin, D., Lu, Y., Leif, R. C., Yang, S., Rajendran, M., & Miller, L. W. Current Protocols in Cytometry / Editorial Board, J. Paul Robinson, Managing Editor ‥. [et Al.], 67:Unit 2.22., 2014. doi abstract bibtex The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.
@article{ jin_how_2014,
title = {How to build a time-gated luminescence microscope},
volume = {67},
issn = {1934-9300},
doi = {10.1002/0471142956.cy0222s67},
abstract = {The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.},
language = {eng},
journal = {Current Protocols in Cytometry / Editorial Board, J. Paul Robinson, Managing Editor ‥. [et Al.]},
author = {Jin, Dayong and Lu, Yiqing and Leif, Robert C. and Yang, Sean and Rajendran, Megha and Miller, Lawrence W.},
year = {2014},
pmid = {24510771},
pages = {Unit 2.22.}
}
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