Detection of Chalara Fraxinea from Tissue of Fraxinus Excelsior Using Species-Specific ITS Primers. Johansson, S. B. K., Vasaitis, R., Ihrmark, K., Barklund, P., & Stenlid, J. 40(2):111–115.
Detection of Chalara Fraxinea from Tissue of Fraxinus Excelsior Using Species-Specific ITS Primers [link]Paper  doi  abstract   bibtex   
Chalara fraxinea (teleomorph: Hymenoscyphus albidus) is known as a serious pathogen of Fraxinus excelsior, causing massive dieback of trees in Europe. The fungus is able to cause latent infections, and has been previously detected as an endophyte in asymptomatic tissues. Chalara fraxinea is a slow grower in culture, and is thus likely to be overgrown by faster growing fungi whenever pure culture isolations are being attempted. This study reports species-specific ITS primers allowing fast and reliable detection of the pathogen directly from infected tissues of F. excelsior.
@article{johanssonDetectionChalaraFraxinea2010,
  title = {Detection of {{Chalara}} Fraxinea from Tissue of {{Fraxinus}} Excelsior Using Species-Specific {{ITS}} Primers},
  author = {Johansson, S. B. K. and Vasaitis, R. and Ihrmark, K. and Barklund, P. and Stenlid, J.},
  date = {2010-04},
  journaltitle = {Forest Pathology},
  volume = {40},
  pages = {111--115},
  issn = {1437-4781},
  doi = {10.1111/j.1439-0329.2009.00614.x},
  url = {https://doi.org/10.1111/j.1439-0329.2009.00614.x},
  abstract = {Chalara fraxinea (teleomorph: Hymenoscyphus albidus) is known as a serious pathogen of Fraxinus excelsior, causing massive dieback of trees in Europe. The fungus is able to cause latent infections, and has been previously detected as an endophyte in asymptomatic tissues. Chalara fraxinea is a slow grower in culture, and is thus likely to be overgrown by faster growing fungi whenever pure culture isolations are being attempted. This study reports species-specific ITS primers allowing fast and reliable detection of the pathogen directly from infected tissues of F. excelsior.},
  keywords = {*imported-from-citeulike-INRMM,~INRMM-MiD:c-14110749,~to-add-doi-URL,chalara-fraxinea,forest-pests,forest-resources,fraxinus-excelsior},
  number = {2}
}
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