Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients. Jugwanth, S., Gededzha, M. P, Mampeule, N., Zwane, N., David, A., Burgers, W. A, Blackburn, J. M, Grove, J. S, George, J. A, Sanne, I., Scott, L., Stevens, W., & Mayne, E. S PLOS ONE, 17(2):e0262442, Public Library of Science, feb, 2022. ![Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex In late December 2019, pneumonia cases of unknown origin were reported in Wuhan, China. This virus was named SARS-CoV2 and the clinical syndrome was named coronavirus disease 19 (COVID-19). South Africa, despite strict and early lockdown has the highest infection rate in Africa. A key component of South Africa's response to SARSCoV2 was the rapid scale-up of diagnostic testing. The Abbott SARS-CoV2 assay detects IgG antibodies against the Nucleocapsid (N) protein of the SARS-CoV2 virus. This study undertook to validate and evaluate performance criteria of the Abbott assay and to establish whether this assay would show clinical utility in our population. Positive patients (n = 391) and negative controls (n = 139) were included. The Architect-i and Alinity-i systems were analyzers that were used to perform the SARS-CoV-2 IgG assay. In-house ELISA was incorporated into the study as a confirmatory serology test. A total of number of 530 participants was tested, 87% were symptomatic with infection and 13% were asymptomatic. When compared to RT-qPCR, the sensitivity of Architect and Alinity SARS-CoV2 assays was 69.5% and 64.8%, respectively. Specificity for Architect and Alinity assays was 95% and 90.3%, respectively. The Abbott assay was also compared to in house ELISA assay, with sensitivity for the Architect and Alinity assays of 94.7% and 92.5%, respectively. Specificity for Abbott Alinity assays was 91.7% higher than Abbott Architect 88.1%. Based on the current findings testing of IgG after 14 days is recommended in South Africa and supports other studies performed around the world.
@article{Jugwanth2022,
abstract = {In late December 2019, pneumonia cases of unknown origin were reported in Wuhan, China. This virus was named SARS-CoV2 and the clinical syndrome was named coronavirus disease 19 (COVID-19). South Africa, despite strict and early lockdown has the highest infection rate in Africa. A key component of South Africa's response to SARSCoV2 was the rapid scale-up of diagnostic testing. The Abbott SARS-CoV2 assay detects IgG antibodies against the Nucleocapsid (N) protein of the SARS-CoV2 virus. This study undertook to validate and evaluate performance criteria of the Abbott assay and to establish whether this assay would show clinical utility in our population. Positive patients (n = 391) and negative controls (n = 139) were included. The Architect-i and Alinity-i systems were analyzers that were used to perform the SARS-CoV-2 IgG assay. In-house ELISA was incorporated into the study as a confirmatory serology test. A total of number of 530 participants was tested, 87{\%} were symptomatic with infection and 13{\%} were asymptomatic. When compared to RT-qPCR, the sensitivity of Architect and Alinity SARS-CoV2 assays was 69.5{\%} and 64.8{\%}, respectively. Specificity for Architect and Alinity assays was 95{\%} and 90.3{\%}, respectively. The Abbott assay was also compared to in house ELISA assay, with sensitivity for the Architect and Alinity assays of 94.7{\%} and 92.5{\%}, respectively. Specificity for Abbott Alinity assays was 91.7{\%} higher than Abbott Architect 88.1{\%}. Based on the current findings testing of IgG after 14 days is recommended in South Africa and supports other studies performed around the world.},
author = {Jugwanth, Sarika and Gededzha, Maemu P and Mampeule, Nakampe and Zwane, Nontobeko and David, Anura and Burgers, Wendy A and Blackburn, Jonathan M and Grove, Jurette S and George, Jaya A and Sanne, Ian and Scott, Lesley and Stevens, Wendy and Mayne, Elizabeth S},
doi = {10.1371/JOURNAL.PONE.0262442},
editor = {Wu, Han-Chung},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Jugwanth et al. - 2022 - Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients.pdf:pdf},
isbn = {1111111111},
issn = {1932-6203},
journal = {PLOS ONE},
keywords = {COVID 19,Enzyme-linked immunoassays,OA,OA{\_}PMC,Pandemics,Respiratory infections,SARS,SARS CoV 2,Serology,Virus testing,fund{\_}not{\_}ack,original},
mendeley-tags = {OA,OA{\_}PMC,fund{\_}not{\_}ack,original},
month = {feb},
number = {2},
pages = {e0262442},
pmid = {35120133},
publisher = {Public Library of Science},
title = {{Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients}},
url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262442},
volume = {17},
year = {2022}
}
Downloads: 0
{"_id":"23vM32ZLEXhMWYW4d","bibbaseid":"jugwanth-gededzha-mampeule-zwane-david-burgers-blackburn-grove-etal-performanceoftheabbottsarscov2iggserologicalassayinsouthafrican2patients-2022","author_short":["Jugwanth, S.","Gededzha, M. P","Mampeule, N.","Zwane, N.","David, A.","Burgers, W. A","Blackburn, J. M","Grove, J. S","George, J. A","Sanne, I.","Scott, L.","Stevens, W.","Mayne, E. S"],"bibdata":{"bibtype":"article","type":"article","abstract":"In late December 2019, pneumonia cases of unknown origin were reported in Wuhan, China. This virus was named SARS-CoV2 and the clinical syndrome was named coronavirus disease 19 (COVID-19). South Africa, despite strict and early lockdown has the highest infection rate in Africa. A key component of South Africa's response to SARSCoV2 was the rapid scale-up of diagnostic testing. The Abbott SARS-CoV2 assay detects IgG antibodies against the Nucleocapsid (N) protein of the SARS-CoV2 virus. This study undertook to validate and evaluate performance criteria of the Abbott assay and to establish whether this assay would show clinical utility in our population. Positive patients (n = 391) and negative controls (n = 139) were included. The Architect-i and Alinity-i systems were analyzers that were used to perform the SARS-CoV-2 IgG assay. In-house ELISA was incorporated into the study as a confirmatory serology test. A total of number of 530 participants was tested, 87% were symptomatic with infection and 13% were asymptomatic. When compared to RT-qPCR, the sensitivity of Architect and Alinity SARS-CoV2 assays was 69.5% and 64.8%, respectively. Specificity for Architect and Alinity assays was 95% and 90.3%, respectively. The Abbott assay was also compared to in house ELISA assay, with sensitivity for the Architect and Alinity assays of 94.7% and 92.5%, respectively. Specificity for Abbott Alinity assays was 91.7% higher than Abbott Architect 88.1%. Based on the current findings testing of IgG after 14 days is recommended in South Africa and supports other studies performed around the world.","author":[{"propositions":[],"lastnames":["Jugwanth"],"firstnames":["Sarika"],"suffixes":[]},{"propositions":[],"lastnames":["Gededzha"],"firstnames":["Maemu","P"],"suffixes":[]},{"propositions":[],"lastnames":["Mampeule"],"firstnames":["Nakampe"],"suffixes":[]},{"propositions":[],"lastnames":["Zwane"],"firstnames":["Nontobeko"],"suffixes":[]},{"propositions":[],"lastnames":["David"],"firstnames":["Anura"],"suffixes":[]},{"propositions":[],"lastnames":["Burgers"],"firstnames":["Wendy","A"],"suffixes":[]},{"propositions":[],"lastnames":["Blackburn"],"firstnames":["Jonathan","M"],"suffixes":[]},{"propositions":[],"lastnames":["Grove"],"firstnames":["Jurette","S"],"suffixes":[]},{"propositions":[],"lastnames":["George"],"firstnames":["Jaya","A"],"suffixes":[]},{"propositions":[],"lastnames":["Sanne"],"firstnames":["Ian"],"suffixes":[]},{"propositions":[],"lastnames":["Scott"],"firstnames":["Lesley"],"suffixes":[]},{"propositions":[],"lastnames":["Stevens"],"firstnames":["Wendy"],"suffixes":[]},{"propositions":[],"lastnames":["Mayne"],"firstnames":["Elizabeth","S"],"suffixes":[]}],"doi":"10.1371/JOURNAL.PONE.0262442","editor":[{"propositions":[],"lastnames":["Wu"],"firstnames":["Han-Chung"],"suffixes":[]}],"file":":C$\\$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Jugwanth et al. - 2022 - Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients.pdf:pdf","isbn":"1111111111","issn":"1932-6203","journal":"PLOS ONE","keywords":"COVID 19,Enzyme-linked immunoassays,OA,OA_PMC,Pandemics,Respiratory infections,SARS,SARS CoV 2,Serology,Virus testing,fund_not_ack,original","mendeley-tags":"OA,OA_PMC,fund_not_ack,original","month":"feb","number":"2","pages":"e0262442","pmid":"35120133","publisher":"Public Library of Science","title":"Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients","url":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262442","volume":"17","year":"2022","bibtex":"@article{Jugwanth2022,\r\nabstract = {In late December 2019, pneumonia cases of unknown origin were reported in Wuhan, China. This virus was named SARS-CoV2 and the clinical syndrome was named coronavirus disease 19 (COVID-19). South Africa, despite strict and early lockdown has the highest infection rate in Africa. A key component of South Africa's response to SARSCoV2 was the rapid scale-up of diagnostic testing. The Abbott SARS-CoV2 assay detects IgG antibodies against the Nucleocapsid (N) protein of the SARS-CoV2 virus. This study undertook to validate and evaluate performance criteria of the Abbott assay and to establish whether this assay would show clinical utility in our population. Positive patients (n = 391) and negative controls (n = 139) were included. The Architect-i and Alinity-i systems were analyzers that were used to perform the SARS-CoV-2 IgG assay. In-house ELISA was incorporated into the study as a confirmatory serology test. A total of number of 530 participants was tested, 87{\\%} were symptomatic with infection and 13{\\%} were asymptomatic. When compared to RT-qPCR, the sensitivity of Architect and Alinity SARS-CoV2 assays was 69.5{\\%} and 64.8{\\%}, respectively. Specificity for Architect and Alinity assays was 95{\\%} and 90.3{\\%}, respectively. The Abbott assay was also compared to in house ELISA assay, with sensitivity for the Architect and Alinity assays of 94.7{\\%} and 92.5{\\%}, respectively. Specificity for Abbott Alinity assays was 91.7{\\%} higher than Abbott Architect 88.1{\\%}. Based on the current findings testing of IgG after 14 days is recommended in South Africa and supports other studies performed around the world.},\r\nauthor = {Jugwanth, Sarika and Gededzha, Maemu P and Mampeule, Nakampe and Zwane, Nontobeko and David, Anura and Burgers, Wendy A and Blackburn, Jonathan M and Grove, Jurette S and George, Jaya A and Sanne, Ian and Scott, Lesley and Stevens, Wendy and Mayne, Elizabeth S},\r\ndoi = {10.1371/JOURNAL.PONE.0262442},\r\neditor = {Wu, Han-Chung},\r\nfile = {:C$\\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Jugwanth et al. - 2022 - Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients.pdf:pdf},\r\nisbn = {1111111111},\r\nissn = {1932-6203},\r\njournal = {PLOS ONE},\r\nkeywords = {COVID 19,Enzyme-linked immunoassays,OA,OA{\\_}PMC,Pandemics,Respiratory infections,SARS,SARS CoV 2,Serology,Virus testing,fund{\\_}not{\\_}ack,original},\r\nmendeley-tags = {OA,OA{\\_}PMC,fund{\\_}not{\\_}ack,original},\r\nmonth = {feb},\r\nnumber = {2},\r\npages = {e0262442},\r\npmid = {35120133},\r\npublisher = {Public Library of Science},\r\ntitle = {{Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients}},\r\nurl = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262442},\r\nvolume = {17},\r\nyear = {2022}\r\n}\r\n","author_short":["Jugwanth, S.","Gededzha, M. P","Mampeule, N.","Zwane, N.","David, A.","Burgers, W. A","Blackburn, J. M","Grove, J. S","George, J. A","Sanne, I.","Scott, L.","Stevens, W.","Mayne, E. S"],"editor_short":["Wu, H."],"key":"Jugwanth2022","id":"Jugwanth2022","bibbaseid":"jugwanth-gededzha-mampeule-zwane-david-burgers-blackburn-grove-etal-performanceoftheabbottsarscov2iggserologicalassayinsouthafrican2patients-2022","role":"author","urls":{"Paper":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262442"},"keyword":["COVID 19","Enzyme-linked immunoassays","OA","OA_PMC","Pandemics","Respiratory infections","SARS","SARS CoV 2","Serology","Virus testing","fund_not_ack","original"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://drive.google.com/uc?export=download&id=1-JLqZ7RwZ3VC2d6ErLGHAtOeMRS_7GCz","dataSources":["Krmt6gt9ktB2s6ARh","6GMFi5DCojy3jHY44","zSYPkp7qteCEAzB8y"],"keywords":["covid 19","enzyme-linked immunoassays","oa","oa_pmc","pandemics","respiratory infections","sars","sars cov 2","serology","virus testing","fund_not_ack","original"],"search_terms":["performance","abbott","sars","cov","igg","serological","assay","south","african","patients","jugwanth","gededzha","mampeule","zwane","david","burgers","blackburn","grove","george","sanne","scott","stevens","mayne"],"title":"Performance of the Abbott SARS-CoV-2 IgG serological assay in South African 2 patients","year":2022}