@article{juyo_evaluacion_2011,
	title = {Evaluación de marcadores moleculares asociados con resistencia a gota ({Phytophthora} infestans {L}.) en papas diploides y tetraploides},
	volume = {13},
	copyright = {Copyright (c) 2011 Revista Colombiana de Biotecnología},
	issn = {1909-8758},
	url = {https://revistas.unal.edu.co/index.php/biotecnologia/article/view/27917},
	abstract = {Título en ingles: Evaluation of molecular markers associated with resistance to late blight (Phytophthora infestans L.)  in diploid and tetraploid potatoes   Resumen La papa, cultivo de importancia a nivel mundial es gravemente afectado por gota, enfermedad ocasionada por el oomycete Phytophthora infestans. Actualmente la forma más efectiva para combatir la enfermedad es mediante el desarrollo de cultivares resistentes al patógeno. Para esto, una estrategia es identificar genes que confieran resistencia al patógeno, para lo cual se buscan marcadores asociados con el carácter de resistencia. En este estudio se evaluaron marcadores moleculares tipo SCAR (Sequence Characterized Amplified Region): CosA, GP179, BA47f2 y Prp1 asociados con resistencia a P. infestans y el gen de resistencia R1, en 22 cultivares tetraploides pertenecientes a la subespecie andigena y cinco especies silvestres. Se evaluó el polimorfismo y se determinó si los alelos polimórficos permitían diferenciar genotipos resistentes de susceptibles. Se comparó el  tamaño de los fragmentos obtenidos con los fragmentos esperados asociados con resistencia de acuerdo a reportes. El análisis se realizó considerando presencia/ausencia de los fragmentos: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600,y Prp1900.Los resultadosindicaron que en los cultivares tetraploides y silvestres, se presentaron polimorfismos en todos los marcadores evaluados, con excepción del marcador GP179. No se encontró correlación entre el rasgo de resistencia y los alelos. Los resultados de este estudio muestran que hay repuesta diferencial a los marcadores entre las subsp. tuberosum y subsp. Andigena.   Palabras clave: Phytophthora infestans, resistencia a gota, marcadores diagnóstico, Solanum tuberosum subsp. Andigena. Abstract Potato is an important worldwide crop seriously affected by late blight disease caused by the oomycete Phytophthora infestans. Currently, the most effective way to control the disease is developing resistant cultivars to the pathogen by identifying genes that confer resistance to the pathogen. For this purpose it is important to find molecular markers associated with the trait. In this study, the SCAR (Sequence Characterized Amplified Region) markers: CosA, GP179, BA47f2 y Prp1, associated with late blight and the resistant gen R1 were evaluated in 22 tetraploid cultivars from subspecie andigena and five wild potato species. Polymorphism was evaluated and it was evaluated if polymorphic alleles allow differentiating resistant from susceptible genotypes. The fragment length for each marker was compared to the allele size reported associated to resistance. The analysis considered the presence/absence of the fragments: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600 and Prp1900. The results indicated that both, tetraploid cultivars and wild potatoes, showed polymorphisms with all these markers, except with the GP179 marker. It was not found correlation between resistance and the presence of specific alleles. Evidence found in this study indicates that results obtained with molecular markers differed between subsp. tuberosum and subsp. andigena. Key words: Phytophthora infestans, late blight resistance, diagnostic markers, Solanum tuberosum subsp. andigena.},
	language = {es; en},
	number = {2},
	urldate = {2020-07-02},
	journal = {Revista Colombiana de Biotecnología},
	author = {Juyó, D. K. and Gerena, H. N. and Mosquera, Teresa},
	month = jul,
	year = {2011},
	note = {Number: 2},
	keywords = {Phytophthora infestans, Solanum tuberosum subsp. Andigena, marcadores diagnóstico, resistencia a gota},
	pages = {51--62}
}

{"_id":"rYJ9YC9vRmu56bDC3","bibbaseid":"juy-gerena-mosquera-evaluacindemarcadoresmolecularesasociadosconresistenciaagotaphytophthorainfestanslenpapasdiploidesytetraploides-2011","authorIDs":[],"author_short":["Juyó, D. K.","Gerena, H. N.","Mosquera, T."],"bibdata":{"bibtype":"article","type":"article","title":"Evaluación de marcadores moleculares asociados con resistencia a gota (Phytophthora infestans L.) en papas diploides y tetraploides","volume":"13","copyright":"Copyright (c) 2011 Revista Colombiana de Biotecnología","issn":"1909-8758","url":"https://revistas.unal.edu.co/index.php/biotecnologia/article/view/27917","abstract":"Título en ingles: Evaluation of molecular markers associated with resistance to late blight (Phytophthora infestans L.)  in diploid and tetraploid potatoes Resumen La papa, cultivo de importancia a nivel mundial es gravemente afectado por gota, enfermedad ocasionada por el oomycete Phytophthora infestans. Actualmente la forma más efectiva para combatir la enfermedad es mediante el desarrollo de cultivares resistentes al patógeno. Para esto, una estrategia es identificar genes que confieran resistencia al patógeno, para lo cual se buscan marcadores asociados con el carácter de resistencia. En este estudio se evaluaron marcadores moleculares tipo SCAR (Sequence Characterized Amplified Region): CosA, GP179, BA47f2 y Prp1 asociados con resistencia a P. infestans y el gen de resistencia R1, en 22 cultivares tetraploides pertenecientes a la subespecie andigena y cinco especies silvestres. Se evaluó el polimorfismo y se determinó si los alelos polimórficos permitían diferenciar genotipos resistentes de susceptibles. Se comparó el  tamaño de los fragmentos obtenidos con los fragmentos esperados asociados con resistencia de acuerdo a reportes. El análisis se realizó considerando presencia/ausencia de los fragmentos: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600,y Prp1900.Los resultadosindicaron que en los cultivares tetraploides y silvestres, se presentaron polimorfismos en todos los marcadores evaluados, con excepción del marcador GP179. No se encontró correlación entre el rasgo de resistencia y los alelos. Los resultados de este estudio muestran que hay repuesta diferencial a los marcadores entre las subsp. tuberosum y subsp. Andigena.   Palabras clave: Phytophthora infestans, resistencia a gota, marcadores diagnóstico, Solanum tuberosum subsp. Andigena. Abstract Potato is an important worldwide crop seriously affected by late blight disease caused by the oomycete Phytophthora infestans. Currently, the most effective way to control the disease is developing resistant cultivars to the pathogen by identifying genes that confer resistance to the pathogen. For this purpose it is important to find molecular markers associated with the trait. In this study, the SCAR (Sequence Characterized Amplified Region) markers: CosA, GP179, BA47f2 y Prp1, associated with late blight and the resistant gen R1 were evaluated in 22 tetraploid cultivars from subspecie andigena and five wild potato species. Polymorphism was evaluated and it was evaluated if polymorphic alleles allow differentiating resistant from susceptible genotypes. The fragment length for each marker was compared to the allele size reported associated to resistance. The analysis considered the presence/absence of the fragments: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600 and Prp1900. The results indicated that both, tetraploid cultivars and wild potatoes, showed polymorphisms with all these markers, except with the GP179 marker. It was not found correlation between resistance and the presence of specific alleles. Evidence found in this study indicates that results obtained with molecular markers differed between subsp. tuberosum and subsp. andigena. Key words: Phytophthora infestans, late blight resistance, diagnostic markers, Solanum tuberosum subsp. andigena.","language":"es; en","number":"2","urldate":"2020-07-02","journal":"Revista Colombiana de Biotecnología","author":[{"propositions":[],"lastnames":["Juyó"],"firstnames":["D.","K."],"suffixes":[]},{"propositions":[],"lastnames":["Gerena"],"firstnames":["H.","N."],"suffixes":[]},{"propositions":[],"lastnames":["Mosquera"],"firstnames":["Teresa"],"suffixes":[]}],"month":"July","year":"2011","note":"Number: 2","keywords":"Phytophthora infestans, Solanum tuberosum subsp. Andigena, marcadores diagnóstico, resistencia a gota","pages":"51–62","bibtex":"@article{juyo_evaluacion_2011,\n\ttitle = {Evaluación de marcadores moleculares asociados con resistencia a gota ({Phytophthora} infestans {L}.) en papas diploides y tetraploides},\n\tvolume = {13},\n\tcopyright = {Copyright (c) 2011 Revista Colombiana de Biotecnología},\n\tissn = {1909-8758},\n\turl = {https://revistas.unal.edu.co/index.php/biotecnologia/article/view/27917},\n\tabstract = {Título en ingles: Evaluation of molecular markers associated with resistance to late blight (Phytophthora infestans L.)  in diploid and tetraploid potatoes Resumen La papa, cultivo de importancia a nivel mundial es gravemente afectado por gota, enfermedad ocasionada por el oomycete Phytophthora infestans. Actualmente la forma más efectiva para combatir la enfermedad es mediante el desarrollo de cultivares resistentes al patógeno. Para esto, una estrategia es identificar genes que confieran resistencia al patógeno, para lo cual se buscan marcadores asociados con el carácter de resistencia. En este estudio se evaluaron marcadores moleculares tipo SCAR (Sequence Characterized Amplified Region): CosA, GP179, BA47f2 y Prp1 asociados con resistencia a P. infestans y el gen de resistencia R1, en 22 cultivares tetraploides pertenecientes a la subespecie andigena y cinco especies silvestres. Se evaluó el polimorfismo y se determinó si los alelos polimórficos permitían diferenciar genotipos resistentes de susceptibles. Se comparó el  tamaño de los fragmentos obtenidos con los fragmentos esperados asociados con resistencia de acuerdo a reportes. El análisis se realizó considerando presencia/ausencia de los fragmentos: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600,y Prp1900.Los resultadosindicaron que en los cultivares tetraploides y silvestres, se presentaron polimorfismos en todos los marcadores evaluados, con excepción del marcador GP179. No se encontró correlación entre el rasgo de resistencia y los alelos. Los resultados de este estudio muestran que hay repuesta diferencial a los marcadores entre las subsp. tuberosum y subsp. Andigena.   Palabras clave: Phytophthora infestans, resistencia a gota, marcadores diagnóstico, Solanum tuberosum subsp. Andigena. Abstract Potato is an important worldwide crop seriously affected by late blight disease caused by the oomycete Phytophthora infestans. Currently, the most effective way to control the disease is developing resistant cultivars to the pathogen by identifying genes that confer resistance to the pathogen. For this purpose it is important to find molecular markers associated with the trait. In this study, the SCAR (Sequence Characterized Amplified Region) markers: CosA, GP179, BA47f2 y Prp1, associated with late blight and the resistant gen R1 were evaluated in 22 tetraploid cultivars from subspecie andigena and five wild potato species. Polymorphism was evaluated and it was evaluated if polymorphic alleles allow differentiating resistant from susceptible genotypes. The fragment length for each marker was compared to the allele size reported associated to resistance. The analysis considered the presence/absence of the fragments: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600 and Prp1900. The results indicated that both, tetraploid cultivars and wild potatoes, showed polymorphisms with all these markers, except with the GP179 marker. It was not found correlation between resistance and the presence of specific alleles. Evidence found in this study indicates that results obtained with molecular markers differed between subsp. tuberosum and subsp. andigena. Key words: Phytophthora infestans, late blight resistance, diagnostic markers, Solanum tuberosum subsp. andigena.},\n\tlanguage = {es; en},\n\tnumber = {2},\n\turldate = {2020-07-02},\n\tjournal = {Revista Colombiana de Biotecnología},\n\tauthor = {Juyó, D. K. and Gerena, H. N. and Mosquera, Teresa},\n\tmonth = jul,\n\tyear = {2011},\n\tnote = {Number: 2},\n\tkeywords = {Phytophthora infestans, Solanum tuberosum subsp. Andigena, marcadores diagnóstico, resistencia a gota},\n\tpages = {51--62}\n}\n\n","author_short":["Juyó, D. K.","Gerena, H. 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