Comprehensive steroid profiling by liquid chromatography coupled to high resolution mass spectrometry

Comprehensive steroid profiling by liquid chromatography coupled to high resolution mass spectrometry. Kaabia, Z., Laparre, J., Cesbron, N., Le Bizec, B., & Dervilly-Pinel, G. 183:106–115.

Paper doi abstract bibtex

A steroidomics workﬂow has been developed in the objective of monitoring a wide range (n \textgreater 150) of steroids in urine. The proposed workﬂow relies on the optimization of an adequate SPE extraction step followed by an UHPLC-HRMS/MS simultaneous analysis of both free and conjugated forms of C18, C19 and C21 steroid hormones. On the basis of 44 selected steroids, representative of main classes of steroids constituting the steroidome, the performances of the developed workﬂow were evaluated in terms of selectivity, repeatability (\textless 13%) and linearity (R2 \textgreater 0.985 in the concentration range [0.01–10 ng/mL]). As metabolites identiﬁcation and characterization constitute the bottleneck of such proﬁling approaches, a homemade database was created encompassing a large number of characterized free and conjugated steroids (n \textgreater 150) for putative steroid-like biomarkers identiﬁcation purposes. The eﬃciency of the workﬂow in highlighting ﬁne modiﬁcations within the urinary steroidome was assessed in the frame of an anabolic treatment involving an intra-muscular administration of boldenone undecylenate (2 mg/kg) to veals (n=6) and the investigation of potential steroid biomarkers. Besides monitoring known phase II metabolites of boldenone in the bovine specie, namely, boldenone glucuronide and sulfate, the applied strategy also permitted to observe, upon boldenone administration, a modiﬁed proﬁle of epiboldenone glucuronide. Furthermore, 31 signals corresponding to non-identiﬁed steroid species could also be highlighted as impacted upon the exogenous steroid treatment. This study is the ﬁrst to simultaneously investigate both free and conjugated C18, C19 and C21 steroid hormones in their native form using UHPLC-HRMS/MS and allowing their comprehensive proﬁling. This strategy was probed in-vivo.

@article{kaabia_comprehensive_2018,
	title = {Comprehensive steroid profiling by liquid chromatography coupled to high resolution mass spectrometry},
	volume = {183},
	issn = {09600760},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S096007601830325X},
	doi = {10.1016/j.jsbmb.2018.06.003},
	abstract = {A steroidomics workﬂow has been developed in the objective of monitoring a wide range (n {\textgreater} 150) of steroids in urine. The proposed workﬂow relies on the optimization of an adequate {SPE} extraction step followed by an {UHPLC}-{HRMS}/{MS} simultaneous analysis of both free and conjugated forms of C18, C19 and C21 steroid hormones. On the basis of 44 selected steroids, representative of main classes of steroids constituting the steroidome, the performances of the developed workﬂow were evaluated in terms of selectivity, repeatability ({\textless} 13\%) and linearity (R2 {\textgreater} 0.985 in the concentration range [0.01–10 ng/{mL}]). As metabolites identiﬁcation and characterization constitute the bottleneck of such proﬁling approaches, a homemade database was created encompassing a large number of characterized free and conjugated steroids (n {\textgreater} 150) for putative steroid-like biomarkers identiﬁcation purposes. The eﬃciency of the workﬂow in highlighting ﬁne modiﬁcations within the urinary steroidome was assessed in the frame of an anabolic treatment involving an intra-muscular administration of boldenone undecylenate (2 mg/kg) to veals (n=6) and the investigation of potential steroid biomarkers. Besides monitoring known phase {II} metabolites of boldenone in the bovine specie, namely, boldenone glucuronide and sulfate, the applied strategy also permitted to observe, upon boldenone administration, a modiﬁed proﬁle of epiboldenone glucuronide. Furthermore, 31 signals corresponding to non-identiﬁed steroid species could also be highlighted as impacted upon the exogenous steroid treatment. This study is the ﬁrst to simultaneously investigate both free and conjugated C18, C19 and C21 steroid hormones in their native form using {UHPLC}-{HRMS}/{MS} and allowing their comprehensive proﬁling. This strategy was probed in-vivo.},
	pages = {106--115},
	journaltitle = {The Journal of Steroid Biochemistry and Molecular Biology},
	author = {Kaabia, Zied and Laparre, Jérôme and Cesbron, Nora and Le Bizec, Bruno and Dervilly-Pinel, Gaud},
	urldate = {2019-03-29},
	date = {2018-10},
	langid = {english},
	file = {Kaabia et al. - 2018 - Comprehensive steroid profiling by liquid chromato.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\TAK3LBH7\\Kaabia et al. - 2018 - Comprehensive steroid profiling by liquid chromato.pdf:application/pdf}
}

Downloads: 0

{"_id":"n76Q3ZdrZJ79Ty2mb","bibbaseid":"kaabia-laparre-cesbron-lebizec-dervillypinel-comprehensivesteroidprofilingbyliquidchromatographycoupledtohighresolutionmassspectrometry","downloads":0,"creationDate":"2019-03-29T15:39:59.156Z","title":"Comprehensive steroid profiling by liquid chromatography coupled to high resolution mass spectrometry","author_short":["Kaabia, Z.","Laparre, J.","Cesbron, N.","Le Bizec, B.","Dervilly-Pinel, G."],"year":null,"bibtype":"article","biburl":"https://yguitton.github.io/biblio/mylibrary.bib","bibdata":{"bibtype":"article","type":"article","title":"Comprehensive steroid profiling by liquid chromatography coupled to high resolution mass spectrometry","volume":"183","issn":"09600760","url":"https://linkinghub.elsevier.com/retrieve/pii/S096007601830325X","doi":"10.1016/j.jsbmb.2018.06.003","abstract":"A steroidomics workﬂow has been developed in the objective of monitoring a wide range (n \\textgreater 150) of steroids in urine. The proposed workﬂow relies on the optimization of an adequate SPE extraction step followed by an UHPLC-HRMS/MS simultaneous analysis of both free and conjugated forms of C18, C19 and C21 steroid hormones. On the basis of 44 selected steroids, representative of main classes of steroids constituting the steroidome, the performances of the developed workﬂow were evaluated in terms of selectivity, repeatability (\\textless 13%) and linearity (R2 \\textgreater 0.985 in the concentration range [0.01–10 ng/mL]). As metabolites identiﬁcation and characterization constitute the bottleneck of such proﬁling approaches, a homemade database was created encompassing a large number of characterized free and conjugated steroids (n \\textgreater 150) for putative steroid-like biomarkers identiﬁcation purposes. The eﬃciency of the workﬂow in highlighting ﬁne modiﬁcations within the urinary steroidome was assessed in the frame of an anabolic treatment involving an intra-muscular administration of boldenone undecylenate (2 mg/kg) to veals (n=6) and the investigation of potential steroid biomarkers. Besides monitoring known phase II metabolites of boldenone in the bovine specie, namely, boldenone glucuronide and sulfate, the applied strategy also permitted to observe, upon boldenone administration, a modiﬁed proﬁle of epiboldenone glucuronide. Furthermore, 31 signals corresponding to non-identiﬁed steroid species could also be highlighted as impacted upon the exogenous steroid treatment. This study is the ﬁrst to simultaneously investigate both free and conjugated C18, C19 and C21 steroid hormones in their native form using UHPLC-HRMS/MS and allowing their comprehensive proﬁling. This strategy was probed in-vivo.","pages":"106–115","journaltitle":"The Journal of Steroid Biochemistry and Molecular Biology","author":[{"propositions":[],"lastnames":["Kaabia"],"firstnames":["Zied"],"suffixes":[]},{"propositions":[],"lastnames":["Laparre"],"firstnames":["Jérôme"],"suffixes":[]},{"propositions":[],"lastnames":["Cesbron"],"firstnames":["Nora"],"suffixes":[]},{"propositions":[],"lastnames":["Le","Bizec"],"firstnames":["Bruno"],"suffixes":[]},{"propositions":[],"lastnames":["Dervilly-Pinel"],"firstnames":["Gaud"],"suffixes":[]}],"urldate":"2019-03-29","date":"2018-10","langid":"english","file":"Kaabia et al. - 2018 - Comprehensive steroid profiling by liquid chromato.pdf:C\\:\\\\Users\\\\ygu\\\\Documents\\¶CPOR066_YGU\\\\YGU\\\\Zotero\\\\storage\\\\TAK3LBH7\\\\Kaabia et al. - 2018 - Comprehensive steroid profiling by liquid chromato.pdf:application/pdf","bibtex":"@article{kaabia_comprehensive_2018,\n\ttitle = {Comprehensive steroid profiling by liquid chromatography coupled to high resolution mass spectrometry},\n\tvolume = {183},\n\tissn = {09600760},\n\turl = {https://linkinghub.elsevier.com/retrieve/pii/S096007601830325X},\n\tdoi = {10.1016/j.jsbmb.2018.06.003},\n\tabstract = {A steroidomics workﬂow has been developed in the objective of monitoring a wide range (n {\\textgreater} 150) of steroids in urine. The proposed workﬂow relies on the optimization of an adequate {SPE} extraction step followed by an {UHPLC}-{HRMS}/{MS} simultaneous analysis of both free and conjugated forms of C18, C19 and C21 steroid hormones. On the basis of 44 selected steroids, representative of main classes of steroids constituting the steroidome, the performances of the developed workﬂow were evaluated in terms of selectivity, repeatability ({\\textless} 13\\%) and linearity (R2 {\\textgreater} 0.985 in the concentration range [0.01–10 ng/{mL}]). As metabolites identiﬁcation and characterization constitute the bottleneck of such proﬁling approaches, a homemade database was created encompassing a large number of characterized free and conjugated steroids (n {\\textgreater} 150) for putative steroid-like biomarkers identiﬁcation purposes. The eﬃciency of the workﬂow in highlighting ﬁne modiﬁcations within the urinary steroidome was assessed in the frame of an anabolic treatment involving an intra-muscular administration of boldenone undecylenate (2 mg/kg) to veals (n=6) and the investigation of potential steroid biomarkers. Besides monitoring known phase {II} metabolites of boldenone in the bovine specie, namely, boldenone glucuronide and sulfate, the applied strategy also permitted to observe, upon boldenone administration, a modiﬁed proﬁle of epiboldenone glucuronide. Furthermore, 31 signals corresponding to non-identiﬁed steroid species could also be highlighted as impacted upon the exogenous steroid treatment. This study is the ﬁrst to simultaneously investigate both free and conjugated C18, C19 and C21 steroid hormones in their native form using {UHPLC}-{HRMS}/{MS} and allowing their comprehensive proﬁling. This strategy was probed in-vivo.},\n\tpages = {106--115},\n\tjournaltitle = {The Journal of Steroid Biochemistry and Molecular Biology},\n\tauthor = {Kaabia, Zied and Laparre, Jérôme and Cesbron, Nora and Le Bizec, Bruno and Dervilly-Pinel, Gaud},\n\turldate = {2019-03-29},\n\tdate = {2018-10},\n\tlangid = {english},\n\tfile = {Kaabia et al. - 2018 - Comprehensive steroid profiling by liquid chromato.pdf:C\\:\\\\Users\\\\ygu\\\\Documents\\\\PCPOR066_YGU\\\\YGU\\\\Zotero\\\\storage\\\\TAK3LBH7\\\\Kaabia et al. - 2018 - Comprehensive steroid profiling by liquid chromato.pdf:application/pdf}\n}\n\n","author_short":["Kaabia, Z.","Laparre, J.","Cesbron, N.","Le Bizec, B.","Dervilly-Pinel, G."],"key":"kaabia_comprehensive_2018","id":"kaabia_comprehensive_2018","bibbaseid":"kaabia-laparre-cesbron-lebizec-dervillypinel-comprehensivesteroidprofilingbyliquidchromatographycoupledtohighresolutionmassspectrometry","role":"author","urls":{"Paper":"https://linkinghub.elsevier.com/retrieve/pii/S096007601830325X"},"metadata":{"authorlinks":{}},"html":""},"search_terms":["comprehensive","steroid","profiling","liquid","chromatography","coupled","high","resolution","mass","spectrometry","kaabia","laparre","cesbron","le bizec","dervilly-pinel"],"keywords":[],"authorIDs":[],"dataSources":["npZbB8XqBLCP4Micr"]}