@article{
 title = {A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro-cellular polymers},
 type = {article},
 year = {2009},
 identifiers = {[object Object]},
 pages = {511-518},
 volume = {84},
 id = {d64eb882-671c-3f96-be37-a8db8a62c867},
 created = {2016-04-28T08:46:17.000Z},
 file_attached = {false},
 profile_id = {dce7c6b2-57cf-350f-b364-3e8ed99bb344},
 group_id = {bfd80d76-e42d-36f1-b5b9-353e1a47eb95},
 last_modified = {2016-04-28T08:46:17.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {ISI:000264946500006},
 source_type = {article},
 abstract = {BACKGROUND: A biochemical oxygen demand (BOD) sensor, based on an
immobilized Pseudomonas syringae in highly porous micro-cellular
polymer (MCP) in combination with a dissolved oxygen electrode, has
been developed for the analysis of biodegradable organic compounds in
aqueous samples. Microorganisms were immobilized in a molded MCP disk
and a wastewater sample was injected into the biocomposite disk by a
flow injection system. Dissolved oxygen (DO) changes as a measure of
soluble BOD was read with a DO probe placed into a flow cell carrying
biocatalytically activated disk.
RESULTS: Optimal response of the MCP BOD sensor was obtained at pH 6.8
and 25 degrees C with a typical response time of 3-5 min for a 2 mm
thick molded polymeric disk. The sensor showed detection linearity over
the range 5-100 mg L-1 BOD5 (r(2) > 0.99) at a flow rate of 0.6 mL
min(-1). The repeatability and reproducibility of the sensor response
were found to be 3.08% and 7.77%, respectively. BOD values produced
with this biosensor for various municipal and industrial wastewaters
correlated well with those determined by the conventional 5-day BOD
test.
CONCLUSION: This new biosensor was different from present amperometric
BOD biosensor configurations in which the biocatalyst
(microbial/enzymatic) is placed between cellulose and Teflon membranes
installed on a DO probe. The use of a molded MCP disk coniainng
microbial activity offers better stability and lifetime for commercial
use in environmental monitoring. (c) 2008 Society of Chemical Industry},
 bibtype = {article},
 author = {Kara, Serdar and Keskinler, Buelent and Erhan, Elif},
 journal = {JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY},
 number = {4}
}

{"_id":"CkoXqwFiqhamx5xc5","bibbaseid":"kara-keskinler-erhan-anovelmicrobialbodbiosensordevelopedbytheimmobilizationofpsyringaeinmicrocellularpolymers-2009","downloads":0,"creationDate":"2016-04-28T08:42:22.955Z","title":"A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro-cellular polymers","author_short":["Kara, S.","Keskinler, B.","Erhan, E."],"year":2009,"bibtype":"article","biburl":null,"bibdata":{"title":"A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro-cellular polymers","type":"article","year":"2009","identifiers":"[object Object]","pages":"511-518","volume":"84","id":"d64eb882-671c-3f96-be37-a8db8a62c867","created":"2016-04-28T08:46:17.000Z","file_attached":false,"profile_id":"dce7c6b2-57cf-350f-b364-3e8ed99bb344","group_id":"bfd80d76-e42d-36f1-b5b9-353e1a47eb95","last_modified":"2016-04-28T08:46:17.000Z","read":false,"starred":false,"authored":false,"confirmed":"true","hidden":false,"citation_key":"ISI:000264946500006","source_type":"article","abstract":"BACKGROUND: A biochemical oxygen demand (BOD) sensor, based on an\nimmobilized Pseudomonas syringae in highly porous micro-cellular\npolymer (MCP) in combination with a dissolved oxygen electrode, has\nbeen developed for the analysis of biodegradable organic compounds in\naqueous samples. Microorganisms were immobilized in a molded MCP disk\nand a wastewater sample was injected into the biocomposite disk by a\nflow injection system. Dissolved oxygen (DO) changes as a measure of\nsoluble BOD was read with a DO probe placed into a flow cell carrying\nbiocatalytically activated disk.\nRESULTS: Optimal response of the MCP BOD sensor was obtained at pH 6.8\nand 25 degrees C with a typical response time of 3-5 min for a 2 mm\nthick molded polymeric disk. The sensor showed detection linearity over\nthe range 5-100 mg L-1 BOD5 (r(2) > 0.99) at a flow rate of 0.6 mL\nmin(-1). The repeatability and reproducibility of the sensor response\nwere found to be 3.08% and 7.77%, respectively. BOD values produced\nwith this biosensor for various municipal and industrial wastewaters\ncorrelated well with those determined by the conventional 5-day BOD\ntest.\nCONCLUSION: This new biosensor was different from present amperometric\nBOD biosensor configurations in which the biocatalyst\n(microbial/enzymatic) is placed between cellulose and Teflon membranes\ninstalled on a DO probe. The use of a molded MCP disk coniainng\nmicrobial activity offers better stability and lifetime for commercial\nuse in environmental monitoring. (c) 2008 Society of Chemical Industry","bibtype":"article","author":"Kara, Serdar and Keskinler, Buelent and Erhan, Elif","journal":"JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY","number":"4","bibtex":"@article{\n title = {A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro-cellular polymers},\n type = {article},\n year = {2009},\n identifiers = {[object Object]},\n pages = {511-518},\n volume = {84},\n id = {d64eb882-671c-3f96-be37-a8db8a62c867},\n created = {2016-04-28T08:46:17.000Z},\n file_attached = {false},\n profile_id = {dce7c6b2-57cf-350f-b364-3e8ed99bb344},\n group_id = {bfd80d76-e42d-36f1-b5b9-353e1a47eb95},\n last_modified = {2016-04-28T08:46:17.000Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n citation_key = {ISI:000264946500006},\n source_type = {article},\n abstract = {BACKGROUND: A biochemical oxygen demand (BOD) sensor, based on an\nimmobilized Pseudomonas syringae in highly porous micro-cellular\npolymer (MCP) in combination with a dissolved oxygen electrode, has\nbeen developed for the analysis of biodegradable organic compounds in\naqueous samples. Microorganisms were immobilized in a molded MCP disk\nand a wastewater sample was injected into the biocomposite disk by a\nflow injection system. Dissolved oxygen (DO) changes as a measure of\nsoluble BOD was read with a DO probe placed into a flow cell carrying\nbiocatalytically activated disk.\nRESULTS: Optimal response of the MCP BOD sensor was obtained at pH 6.8\nand 25 degrees C with a typical response time of 3-5 min for a 2 mm\nthick molded polymeric disk. The sensor showed detection linearity over\nthe range 5-100 mg L-1 BOD5 (r(2) > 0.99) at a flow rate of 0.6 mL\nmin(-1). The repeatability and reproducibility of the sensor response\nwere found to be 3.08% and 7.77%, respectively. BOD values produced\nwith this biosensor for various municipal and industrial wastewaters\ncorrelated well with those determined by the conventional 5-day BOD\ntest.\nCONCLUSION: This new biosensor was different from present amperometric\nBOD biosensor configurations in which the biocatalyst\n(microbial/enzymatic) is placed between cellulose and Teflon membranes\ninstalled on a DO probe. The use of a molded MCP disk coniainng\nmicrobial activity offers better stability and lifetime for commercial\nuse in environmental monitoring. (c) 2008 Society of Chemical Industry},\n bibtype = {article},\n author = {Kara, Serdar and Keskinler, Buelent and Erhan, Elif},\n journal = {JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY},\n number = {4}\n}","author_short":["Kara, S.","Keskinler, B.","Erhan, E."],"bibbaseid":"kara-keskinler-erhan-anovelmicrobialbodbiosensordevelopedbytheimmobilizationofpsyringaeinmicrocellularpolymers-2009","role":"author","urls":{},"downloads":0,"html":""},"search_terms":["novel","microbial","bod","biosensor","developed","immobilization","syringae","micro","cellular","polymers","kara","keskinler","erhan"],"keywords":[],"authorIDs":[]}