@ARTICLE{Karelov20031238,
author={Karelov, D.V. and Krasikov, V.V. and Surzhik, M.A. and Firsov, L.M.},
title={Expression, isolation, purification, and biochemical properties of trehalose-6-phosphate hydrolase from thermoresistant strain Bacillus sp. GP16},
journal={Biokhimiya},
year={2003},
volume={68},
number={9},
pages={1238-1246},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0346151621&partnerID=40&md5=7e1598371f69b6b4fb4a6639cc1bd935},
affiliation={Inst. of Nuclear Physics, RAS, Gatchina, Leningrad Region, 188350, Russian Federation},
abstract={In the paper, cloning, expression, and purification of the enzyme trehalose-6-phosphate hydrolase from thermoresistant strain Bacillus sp. GP16 is described. Principal biochemical properties of the enzyme at different pH and temperature values were determined. Entropy and enthalpy of activation of the enzyme for substrates trehalose-6-phosphate and p-nitrophenyl glucoside were calculated, and the dependence of the kinetic parameters from ionic strength was established.},
author_keywords={Catalysis;  Cloning of a gene;  Determination of Michaelis constants;  Gene expression;  Temperature dependence;  Trehalose-6-phosphate hydrolase},
correspondence_address1={Karelov, D.V.; Inst. of Nuclear Physics, RAS, Gatchina, Leningrad Region, 188350, Russian Federation},
issn={03209725},
coden={BIOHA},
language={Russian},
abbrev_source_title={Biokhim.},
document_type={Article},
source={Scopus},
}

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