Sequencing of the Francisella tularensis Strain Schu 4 Genome Reveals the Shikimate and Purine Metabolic Pathways, Targets for the Construction of a Rationally Attenuated Auxotrophic Vaccine. Karlsson, J., Prior, R. G., Williams, K., Lindler, L., Brown, K. A., Chatwell, N., Hjalmarsson, K., Loman, N., Mack, K. A., Pallen, M., Popek, M., Sandström, G., Sjöstedt, A., Svensson, T., Tamas, I., Andersson, S. G. E., Wren, B. W., Oyston, P. C. F., & Titball, R. W. Microbial & Comparative Genomics, 5(1):25–39, March, 2000. Publisher: Mary Ann Liebert, Inc., publishers![Sequencing of the Francisella tularensis Strain Schu 4 Genome Reveals the Shikimate and Purine Metabolic Pathways, Targets for the Construction of a Rationally Attenuated Auxotrophic Vaccine [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Francisella tularensis is the etiological agent of tularemia, a serious disease in several Northern hemisphere countries. The organism has fastidious growth requirements and is very poorly understood at the genetic and molecular levels. Given the lack of data on this organism, we undertook the sample sequencing of its genome. A random library of DNA fragments from a highly virulent strain (Schu 4) of F. tularensis was constructed and the nucleotide sequences of 13,904 cloned fragments were determined and assembled into 353 contigs. A total of 1.83 Mb of nucleotide sequence was obtained that had a G + C content of 33.2%. Genes located on plasmids pOM1 and pNFL10, which had been previously isolated from low virulence strains of F. tularensis, were absent but all of the other known F. tularensis genes were represented in the assembled data. F. tularensis Schu4 was able to grow in the absence of aromatic amino acids and orthologues of genes which could encode enzymes in the shikimate pathway in other bacteria were identified in the assembled data. Genes that could encode all of the enzymes in the purine biosynthetic and most of the enzymes in the purine salvage pathways were also identified. This data will be used to develop defined rationally attenuated mutants of F. tularensis, which could be used as replacements for the existing genetically undefined live vaccine strain.
@article{karlsson_sequencing_2000,
title = {Sequencing of the {Francisella} tularensis {Strain} {Schu} 4 {Genome} {Reveals} the {Shikimate} and {Purine} {Metabolic} {Pathways}, {Targets} for the {Construction} of a {Rationally} {Attenuated} {Auxotrophic} {Vaccine}},
volume = {5},
issn = {1090-6592},
url = {https://www.liebertpub.com/doi/10.1089/10906590050145249},
doi = {10.1089/10906590050145249},
abstract = {Francisella tularensis is the etiological agent of tularemia, a serious disease in several Northern hemisphere countries. The organism has fastidious growth requirements and is very poorly understood at the genetic and molecular levels. Given the lack of data on this organism, we undertook the sample sequencing of its genome. A random library of DNA fragments from a highly virulent strain (Schu 4) of F. tularensis was constructed and the nucleotide sequences of 13,904 cloned fragments were determined and assembled into 353 contigs. A total of 1.83 Mb of nucleotide sequence was obtained that had a G + C content of 33.2\%. Genes located on plasmids pOM1 and pNFL10, which had been previously isolated from low virulence strains of F. tularensis, were absent but all of the other known F. tularensis genes were represented in the assembled data. F. tularensis Schu4 was able to grow in the absence of aromatic amino acids and orthologues of genes which could encode enzymes in the shikimate pathway in other bacteria were identified in the assembled data. Genes that could encode all of the enzymes in the purine biosynthetic and most of the enzymes in the purine salvage pathways were also identified. This data will be used to develop defined rationally attenuated mutants of F. tularensis, which could be used as replacements for the existing genetically undefined live vaccine strain.},
number = {1},
urldate = {2021-11-08},
journal = {Microbial \& Comparative Genomics},
author = {Karlsson, Jan and Prior, Richard G. and Williams, Kerstin and Lindler, Luther and Brown, Katherine A. and Chatwell, Nicola and Hjalmarsson, Karin and Loman, Nick and Mack, Kerri A. and Pallen, Mark and Popek, Michael and Sandström, Gunnar and Sjöstedt, Anders and Svensson, Thomas and Tamas, Ivica and Andersson, Siv G. E. and Wren, Brendan W. and Oyston, Petra C. F. and Titball, Richard W.},
month = mar,
year = {2000},
note = {Publisher: Mary Ann Liebert, Inc., publishers},
pages = {25--39},
}
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