Room temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I. Keable, S. M., Kölsch, A., Simon, P. S., Dasgupta, M., Chatterjee, R., Subramanian, S. K., Hussein, R., Ibrahim, M., Kim, I., Bogacz, I., Makita, H., Pham, C. C., Fuller, F. D., Gul, S., Paley, D., Lassalle, L., Sutherlin, K. D., Bhowmick, A., Moriarty, N. W., Young, I. D., Blaschke, J. P., de Lichtenberg, C., Chernev, P., Cheah, M. H., Park, S., Park, G., Kim, J., Lee, S. J., Park, J., Tono, K., Owada, S., Hunter, M. S., Batyuk, A., Oggenfuss, R., Sander, M., Zerdane, S., Ozerov, D., Nass, K., Lemke, H., Mankowsky, R., Brewster, A. S., Messinger, J., Sauter, N. K., Yachandra, V. K., Yano, J., Zouni, A., & Kern, J. Scientific Reports, 11(1):21787, November, 2021. Publisher: Nature Publishing Group![Room temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along the two branches. We have determined the structure of cyanobacterial PS I at room temperature (RT) using femtosecond X-ray pulses from an X-ray free electron laser (XFEL) that shows a clear expansion of the entire protein complex in the direction of the membrane plane, when compared to previous cryogenic structures. This trend was observed by complementary datasets taken at multiple XFEL beamlines. In the RT structure of PS I, we also observe conformational differences between the two branches in the reaction center around the secondary electron acceptors A1A and A1B. The π-stacked Phe residues are rotated with a more parallel orientation in the A-branch and an almost perpendicular confirmation in the B-branch, and the symmetry breaking PsaB-Trp673 is tilted and further away from A1A. These changes increase the asymmetry between the branches and may provide insights into the preferential directionality of electron transfer.
@article{keable_room_2021,
title = {Room temperature {XFEL} crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem {I}},
volume = {11},
copyright = {2021 The Author(s)},
issn = {2045-2322},
url = {https://www.nature.com/articles/s41598-021-00236-3},
doi = {10.1038/s41598-021-00236-3},
abstract = {Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along the two branches. We have determined the structure of cyanobacterial PS I at room temperature (RT) using femtosecond X-ray pulses from an X-ray free electron laser (XFEL) that shows a clear expansion of the entire protein complex in the direction of the membrane plane, when compared to previous cryogenic structures. This trend was observed by complementary datasets taken at multiple XFEL beamlines. In the RT structure of PS I, we also observe conformational differences between the two branches in the reaction center around the secondary electron acceptors A1A and A1B. The π-stacked Phe residues are rotated with a more parallel orientation in the A-branch and an almost perpendicular confirmation in the B-branch, and the symmetry breaking PsaB-Trp673 is tilted and further away from A1A. These changes increase the asymmetry between the branches and may provide insights into the preferential directionality of electron transfer.},
language = {en},
number = {1},
urldate = {2024-10-16},
journal = {Scientific Reports},
author = {Keable, Stephen M. and Kölsch, Adrian and Simon, Philipp S. and Dasgupta, Medhanjali and Chatterjee, Ruchira and Subramanian, Senthil Kumar and Hussein, Rana and Ibrahim, Mohamed and Kim, In-Sik and Bogacz, Isabel and Makita, Hiroki and Pham, Cindy C. and Fuller, Franklin D. and Gul, Sheraz and Paley, Daniel and Lassalle, Louise and Sutherlin, Kyle D. and Bhowmick, Asmit and Moriarty, Nigel W. and Young, Iris D. and Blaschke, Johannes P. and de Lichtenberg, Casper and Chernev, Petko and Cheah, Mun Hon and Park, Sehan and Park, Gisu and Kim, Jangwoo and Lee, Sang Jae and Park, Jaehyun and Tono, Kensuke and Owada, Shigeki and Hunter, Mark S. and Batyuk, Alexander and Oggenfuss, Roland and Sander, Mathias and Zerdane, Serhane and Ozerov, Dmitry and Nass, Karol and Lemke, Henrik and Mankowsky, Roman and Brewster, Aaron S. and Messinger, Johannes and Sauter, Nicholas K. and Yachandra, Vittal K. and Yano, Junko and Zouni, Athina and Kern, Jan},
month = nov,
year = {2021},
note = {Publisher: Nature Publishing Group},
keywords = {Biochemistry, Bioenergetics, Photosynthesis, Structural biology},
pages = {21787},
}
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