@article{keel1992,
        author={Keel,Christoph and Schnider,Ursula and Maurhofer,Monika and Voisard,Christophe and Laville,Jacques and Burger,Ulrich and Wirthner,Philippe J. and Haas,Dieter and Défago,Geneviève},
        year={1992},
        title={Suppression of Root Diseases by Pseudomonas fluorescens CHA0: Importance of the Bacterial Secondary Metabolite 2,4-Diacetylphloroglucinol},
        journal={Molecular Plant-Microbe Interactions},
        volume={5},
        number={1},
        pages={4-13},
        note={ID: unige:131168},
        abstract={Pseudomonas fluorescens strain CHAO suppresses Thielaviopsis basicola-induced black root rot of tobacco and Gaeumannomyces graminis var. tritici-induced take-all of wheat. Strain CHAO produces 2,4-diacetylphloroglucinol, a metabolite with antifungal, antibacterial, and phytotoxic activity. The role of this compound in disease suppression was tested under gnotobiotic conditions. A P. fluorescens mutant, obtained by Tn5 insertion, did not produce 2,4-diacetylphloroglucinol, showed diminished inhibition of T. basicola and of G. g. var. tritici in vitro, and had a reduced suppressive effect on tobacco black root rot and on take-all of wheat, compared with wild-type CHAO. Complementation of the mutant with an 11-kb DNA fragment from a genomic library of wild-type CHAO largely restored production of the metabolite, inhibition of the fungal pathogens in vitro and disease suppression. The Tn5 insertion was physically mapped using a 5.8-kb complementing fragment as a probe. 2,4- Diacetylphloroglucinol was shown to be produced in the rhizosphere of wheat by strain CHAO and by the complemented mutant, but not by the mutant defective in 2,4-diacetylphloroglucinol synthesis. These results support the importance of 2,4-diacetylphloroglucinol production by strain CHAO in the suppression of soilborne plant pathogens in the rhizosphere.},
        keywords={Antibiotics; Biological control; Tn5 mutagenesis},
        
        url={https://archive-ouverte.unige.ch/unige:131168},
}

{"_id":"3yfjQxFngK2Nvt5kj","bibbaseid":"keel-schnider-maurhofer-voisard-laville-burger-wirthner-haas-etal-suppressionofrootdiseasesbypseudomonasfluorescenscha0importanceofthebacterialsecondarymetabolite24diacetylphloroglucinol-1992","author_short":["Keel, C.","Schnider, U.","Maurhofer, M.","Voisard, C.","Laville, J.","Burger, U.","Wirthner, P. J.","Haas, D.","Défago, G."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Keel"],"firstnames":["Christoph"],"suffixes":[]},{"propositions":[],"lastnames":["Schnider"],"firstnames":["Ursula"],"suffixes":[]},{"propositions":[],"lastnames":["Maurhofer"],"firstnames":["Monika"],"suffixes":[]},{"propositions":[],"lastnames":["Voisard"],"firstnames":["Christophe"],"suffixes":[]},{"propositions":[],"lastnames":["Laville"],"firstnames":["Jacques"],"suffixes":[]},{"propositions":[],"lastnames":["Burger"],"firstnames":["Ulrich"],"suffixes":[]},{"propositions":[],"lastnames":["Wirthner"],"firstnames":["Philippe","J."],"suffixes":[]},{"propositions":[],"lastnames":["Haas"],"firstnames":["Dieter"],"suffixes":[]},{"propositions":[],"lastnames":["Défago"],"firstnames":["Geneviève"],"suffixes":[]}],"year":"1992","title":"Suppression of Root Diseases by Pseudomonas fluorescens CHA0: Importance of the Bacterial Secondary Metabolite 2,4-Diacetylphloroglucinol","journal":"Molecular Plant-Microbe Interactions","volume":"5","number":"1","pages":"4-13","note":"ID: unige:131168","abstract":"Pseudomonas fluorescens strain CHAO suppresses Thielaviopsis basicola-induced black root rot of tobacco and Gaeumannomyces graminis var. tritici-induced take-all of wheat. Strain CHAO produces 2,4-diacetylphloroglucinol, a metabolite with antifungal, antibacterial, and phytotoxic activity. The role of this compound in disease suppression was tested under gnotobiotic conditions. A P. fluorescens mutant, obtained by Tn5 insertion, did not produce 2,4-diacetylphloroglucinol, showed diminished inhibition of T. basicola and of G. g. var. tritici in vitro, and had a reduced suppressive effect on tobacco black root rot and on take-all of wheat, compared with wild-type CHAO. Complementation of the mutant with an 11-kb DNA fragment from a genomic library of wild-type CHAO largely restored production of the metabolite, inhibition of the fungal pathogens in vitro and disease suppression. The Tn5 insertion was physically mapped using a 5.8-kb complementing fragment as a probe. 2,4- Diacetylphloroglucinol was shown to be produced in the rhizosphere of wheat by strain CHAO and by the complemented mutant, but not by the mutant defective in 2,4-diacetylphloroglucinol synthesis. These results support the importance of 2,4-diacetylphloroglucinol production by strain CHAO in the suppression of soilborne plant pathogens in the rhizosphere.","keywords":"Antibiotics; Biological control; Tn5 mutagenesis","url":"https://archive-ouverte.unige.ch/unige:131168","bibtex":"@article{keel1992,\n author={Keel,Christoph and Schnider,Ursula and Maurhofer,Monika and Voisard,Christophe and Laville,Jacques and Burger,Ulrich and Wirthner,Philippe J. and Haas,Dieter and Défago,Geneviève},\n year={1992},\n title={Suppression of Root Diseases by Pseudomonas fluorescens CHA0: Importance of the Bacterial Secondary Metabolite 2,4-Diacetylphloroglucinol},\n journal={Molecular Plant-Microbe Interactions},\n volume={5},\n number={1},\n pages={4-13},\n note={ID: unige:131168},\n abstract={Pseudomonas fluorescens strain CHAO suppresses Thielaviopsis basicola-induced black root rot of tobacco and Gaeumannomyces graminis var. tritici-induced take-all of wheat. Strain CHAO produces 2,4-diacetylphloroglucinol, a metabolite with antifungal, antibacterial, and phytotoxic activity. The role of this compound in disease suppression was tested under gnotobiotic conditions. A P. fluorescens mutant, obtained by Tn5 insertion, did not produce 2,4-diacetylphloroglucinol, showed diminished inhibition of T. basicola and of G. g. var. tritici in vitro, and had a reduced suppressive effect on tobacco black root rot and on take-all of wheat, compared with wild-type CHAO. Complementation of the mutant with an 11-kb DNA fragment from a genomic library of wild-type CHAO largely restored production of the metabolite, inhibition of the fungal pathogens in vitro and disease suppression. The Tn5 insertion was physically mapped using a 5.8-kb complementing fragment as a probe. 2,4- Diacetylphloroglucinol was shown to be produced in the rhizosphere of wheat by strain CHAO and by the complemented mutant, but not by the mutant defective in 2,4-diacetylphloroglucinol synthesis. These results support the importance of 2,4-diacetylphloroglucinol production by strain CHAO in the suppression of soilborne plant pathogens in the rhizosphere.},\n keywords={Antibiotics; Biological control; Tn5 mutagenesis},\n \n url={https://archive-ouverte.unige.ch/unige:131168},\n}\n\n","author_short":["Keel, C.","Schnider, U.","Maurhofer, M.","Voisard, C.","Laville, J.","Burger, U.","Wirthner, P. J.","Haas, D.","Défago, G."],"key":"keel1992","id":"keel1992","bibbaseid":"keel-schnider-maurhofer-voisard-laville-burger-wirthner-haas-etal-suppressionofrootdiseasesbypseudomonasfluorescenscha0importanceofthebacterialsecondarymetabolite24diacetylphloroglucinol-1992","role":"author","urls":{"Paper":"https://archive-ouverte.unige.ch/unige:131168"},"keyword":["Antibiotics; Biological control; Tn5 mutagenesis"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/network/files/7omCQCMxvjPCQZfjb","dataSources":["4EGaEr5EKz54T8jGa"],"keywords":["antibiotics; biological control; tn5 mutagenesis"],"search_terms":["suppression","root","diseases","pseudomonas","fluorescens","cha0","importance","bacterial","secondary","metabolite","diacetylphloroglucinol","keel","schnider","maurhofer","voisard","laville","burger","wirthner","haas","défago"],"title":"Suppression of Root Diseases by Pseudomonas fluorescens CHA0: Importance of the Bacterial Secondary Metabolite 2,4-Diacetylphloroglucinol","year":1992}