The flanking sequence contributes to the immobilisation of spermine at the G‐quadruplex in the NHE (nuclease hypersensitivity element) III $_{\textrm{1}}$ of the c‐ <i>Myc</i> promoter. Keniry, M. A. & Owen, E. A. FEBS Letters, 588(10):1949–1954, May, 2014. ![The flanking sequence contributes to the immobilisation of spermine at the G‐quadruplex in the NHE (nuclease hypersensitivity element) III $_{\textrm{1}}$ of the c‐ <i>Myc</i> promoter [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine‐dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G‐quadruplexes formed by sequences of the purine‐rich strand of the c‐ Myc promoter, nuclease hypersensitivity element III 1 (NHE III 1 ). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G‐tracts of the NHE III 1 are present. This suggests structure within the nucleotides flanking the G‐quadruplex has a role in immobilising spermine. , Selective NMR experiments give information on spermine structure and mobility. Negative spermine NOEs observed on association with NHE quadruplexes. TOCSY peak intensities support a quadruplex‐induced spermine conformation change. Parallel chain‐reversal G‐quadruplexes attenuate spermine mobility. Five G‐tracts within the NHE generate the most efficient spermine immobilisation.
@article{keniry_flanking_2014,
title = {The flanking sequence contributes to the immobilisation of spermine at the {G}‐quadruplex in the {NHE} (nuclease hypersensitivity element) {III} $_{\textrm{1}}$ of the c‐ \textit{{Myc}} promoter},
volume = {588},
issn = {0014-5793, 1873-3468},
url = {https://febs.onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.04.003},
doi = {10.1016/j.febslet.2014.04.003},
abstract = {Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine‐dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G‐quadruplexes formed by sequences of the purine‐rich strand of the c‐
Myc
promoter, nuclease hypersensitivity element III
1
(NHE III
1
). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G‐tracts of the NHE III
1
are present. This suggests structure within the nucleotides flanking the G‐quadruplex has a role in immobilising spermine.
,
Selective NMR experiments give information on spermine structure and mobility.
Negative spermine NOEs observed on association with NHE quadruplexes.
TOCSY peak intensities support a quadruplex‐induced spermine conformation change.
Parallel chain‐reversal G‐quadruplexes attenuate spermine mobility.
Five G‐tracts within the NHE generate the most efficient spermine immobilisation.},
language = {en},
number = {10},
urldate = {2023-11-03},
journal = {FEBS Letters},
author = {Keniry, Max A. and Owen, Elisabeth A.},
month = may,
year = {2014},
pages = {1949--1954},
}
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