The Flanking Sequence Contributes to the Immobilisation of Spermine at the G-quadruplex in the NHE (Nuclease Hypersensitivity Element) III \textsubscript1 of the c- \emphMyc Promoter. Keniry, M. A. & Owen, E. A. FEBS Letters, 588(10):1949–1954, May, 2014.
doi  abstract   bibtex   
Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c- Myc promoter, nuclease hypersensitivity element III 1 (NHE III 1 ). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III 1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine. , Selective NMR experiments give information on spermine structure and mobility. Negative spermine NOEs observed on association with NHE quadruplexes. TOCSY peak intensities support a quadruplex-induced spermine conformation change. Parallel chain-reversal G-quadruplexes attenuate spermine mobility. Five G-tracts within the NHE generate the most efficient spermine immobilisation.
@article{keniryFlankingSequenceContributes2014,
  title = {The Flanking Sequence Contributes to the Immobilisation of Spermine at the {{G}}-quadruplex in the {{NHE}} (Nuclease Hypersensitivity Element) {{III}} {\textsubscript{1}} of the c- {{{\emph{Myc}}}} Promoter},
  author = {Keniry, Max A. and Owen, Elisabeth A.},
  year = {2014},
  month = may,
  journal = {FEBS Letters},
  volume = {588},
  number = {10},
  pages = {1949--1954},
  issn = {0014-5793, 1873-3468},
  doi = {10.1016/j.febslet.2014.04.003},
  urldate = {2023-11-03},
  abstract = {Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c-               Myc               promoter, nuclease hypersensitivity element III               1               (NHE III               1               ). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III               1               are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine.                        ,                                                                 Selective NMR experiments give information on spermine structure and mobility.                                                     Negative spermine NOEs observed on association with NHE quadruplexes.                                                     TOCSY peak intensities support a quadruplex-induced spermine conformation change.                                                     Parallel chain-reversal G-quadruplexes attenuate spermine mobility.                                                     Five G-tracts within the NHE generate the most efficient spermine immobilisation.},
  langid = {english},
  file = {E\:\\UBCloud\\Zotero\\storage\\4DQ7E4UU\\keniry2014.pdf.pdf;E\:\\UBCloud\\Zotero\\storage\\QK88ZXN6\\Keniry and Owen - 2014 - The flanking sequence contributes to the immobilis.pdf}
}

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