The Flanking Sequence Contributes to the Immobilisation of Spermine at the G-quadruplex in the NHE (Nuclease Hypersensitivity Element) III \textsubscript1 of the c- \emphMyc Promoter. Keniry, M. A. & Owen, E. A. FEBS Letters, 588(10):1949–1954, May, 2014. doi abstract bibtex Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c- Myc promoter, nuclease hypersensitivity element III 1 (NHE III 1 ). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III 1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine. , Selective NMR experiments give information on spermine structure and mobility. Negative spermine NOEs observed on association with NHE quadruplexes. TOCSY peak intensities support a quadruplex-induced spermine conformation change. Parallel chain-reversal G-quadruplexes attenuate spermine mobility. Five G-tracts within the NHE generate the most efficient spermine immobilisation.
@article{keniryFlankingSequenceContributes2014,
title = {The Flanking Sequence Contributes to the Immobilisation of Spermine at the {{G}}-quadruplex in the {{NHE}} (Nuclease Hypersensitivity Element) {{III}} {\textsubscript{1}} of the c- {{{\emph{Myc}}}} Promoter},
author = {Keniry, Max A. and Owen, Elisabeth A.},
year = {2014},
month = may,
journal = {FEBS Letters},
volume = {588},
number = {10},
pages = {1949--1954},
issn = {0014-5793, 1873-3468},
doi = {10.1016/j.febslet.2014.04.003},
urldate = {2023-11-03},
abstract = {Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c- Myc promoter, nuclease hypersensitivity element III 1 (NHE III 1 ). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III 1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine. , Selective NMR experiments give information on spermine structure and mobility. Negative spermine NOEs observed on association with NHE quadruplexes. TOCSY peak intensities support a quadruplex-induced spermine conformation change. Parallel chain-reversal G-quadruplexes attenuate spermine mobility. Five G-tracts within the NHE generate the most efficient spermine immobilisation.},
langid = {english},
file = {E\:\\UBCloud\\Zotero\\storage\\4DQ7E4UU\\keniry2014.pdf.pdf;E\:\\UBCloud\\Zotero\\storage\\QK88ZXN6\\Keniry and Owen - 2014 - The flanking sequence contributes to the immobilis.pdf}
}
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A."],"bibdata":{"bibtype":"article","type":"article","title":"The Flanking Sequence Contributes to the Immobilisation of Spermine at the G-quadruplex in the NHE (Nuclease Hypersensitivity Element) III \\textsubscript1 of the c- \\emphMyc Promoter","author":[{"propositions":[],"lastnames":["Keniry"],"firstnames":["Max","A."],"suffixes":[]},{"propositions":[],"lastnames":["Owen"],"firstnames":["Elisabeth","A."],"suffixes":[]}],"year":"2014","month":"May","journal":"FEBS Letters","volume":"588","number":"10","pages":"1949–1954","issn":"0014-5793, 1873-3468","doi":"10.1016/j.febslet.2014.04.003","urldate":"2023-11-03","abstract":"Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c- Myc promoter, nuclease hypersensitivity element III 1 (NHE III 1 ). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III 1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine. , Selective NMR experiments give information on spermine structure and mobility. Negative spermine NOEs observed on association with NHE quadruplexes. TOCSY peak intensities support a quadruplex-induced spermine conformation change. Parallel chain-reversal G-quadruplexes attenuate spermine mobility. Five G-tracts within the NHE generate the most efficient spermine immobilisation.","langid":"english","file":"E\\:\\\\UBCloud\\\\Zotero\\\\storage\\\\4DQ7E4UU\\ęniry2014.pdf.pdf;E\\:\\\\UBCloud\\\\Zotero\\\\storage\\\\QK88ZXN6\\\\Keniry and Owen - 2014 - The flanking sequence contributes to the immobilis.pdf","bibtex":"@article{keniryFlankingSequenceContributes2014,\n title = {The Flanking Sequence Contributes to the Immobilisation of Spermine at the {{G}}-quadruplex in the {{NHE}} (Nuclease Hypersensitivity Element) {{III}} {\\textsubscript{1}} of the c- {{{\\emph{Myc}}}} Promoter},\n author = {Keniry, Max A. and Owen, Elisabeth A.},\n year = {2014},\n month = may,\n journal = {FEBS Letters},\n volume = {588},\n number = {10},\n pages = {1949--1954},\n issn = {0014-5793, 1873-3468},\n doi = {10.1016/j.febslet.2014.04.003},\n urldate = {2023-11-03},\n abstract = {Defining the molecular basis of the DNA sequence selectivity of polyamine binding is central to understanding polyamine-dependent gene expression. We have studied, by selective NMR experiments, the variation of spermine mobility and conformation in the presence of G-quadruplexes formed by sequences of the purine-rich strand of the c- Myc promoter, nuclease hypersensitivity element III 1 (NHE III 1 ). All the NHE quadruplexes restrict spermine mobility and induce a spermine conformational change but the most effective immobilisation occurs when all five G-tracts of the NHE III 1 are present. This suggests structure within the nucleotides flanking the G-quadruplex has a role in immobilising spermine. , Selective NMR experiments give information on spermine structure and mobility. Negative spermine NOEs observed on association with NHE quadruplexes. TOCSY peak intensities support a quadruplex-induced spermine conformation change. Parallel chain-reversal G-quadruplexes attenuate spermine mobility. 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A."],"key":"keniryFlankingSequenceContributes2014","id":"keniryFlankingSequenceContributes2014","bibbaseid":"keniry-owen-theflankingsequencecontributestotheimmobilisationofspermineatthegquadruplexinthenhenucleasehypersensitivityelementiiitextsubscript1ofthecemphmycpromoter-2014","role":"author","urls":{},"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/f/F7MP3WMbDyMbtFCC8/My Library.bib","dataSources":["4i5C7S78DvJNsaHyg"],"keywords":[],"search_terms":["flanking","sequence","contributes","immobilisation","spermine","quadruplex","nhe","nuclease","hypersensitivity","element","iii","textsubscript1","emphmyc","promoter","keniry","owen"],"title":"The Flanking Sequence Contributes to the Immobilisation of Spermine at the G-quadruplex in the NHE (Nuclease Hypersensitivity Element) III \\textsubscript1 of the c- \\emphMyc Promoter","year":2014}