Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content?. Kerker, M, Van Dilla, M a, Brunsting, A, Kratohvil, J P, Hsu, P, Wang, D S, Gray, J W, & Langlois, R G Cytometry, 3(2):71--78, 1982. ![Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content? [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 microns) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10% accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.
@article{kerker_is_1982,
title = {Is the central dogma of flow cytometry true: {That} fluorescence intensity is proportional to cellular dye content?},
volume = {3},
issn = {0196-4763},
url = {http://doi.wiley.com/10.1002/cyto.990030202},
doi = {10.1002/cyto.990030202},
abstract = {Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 microns) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10\% accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.},
number = {2},
journal = {Cytometry},
author = {Kerker, M and Van Dilla, M a and Brunsting, A and Kratohvil, J P and Hsu, P and Wang, D S and Gray, J W and Langlois, R G},
year = {1982},
pmid = {7140481},
keywords = {Animals, Biophysical Phenomena, Biophysics, Cells, Flow cytometry, Fluorescent Dyes, microspheres, Particle Size},
pages = {71--78},
file = {Kerker et al_1982_Is the central dogma of flow cytometry true.pdf:/Users/baptiste/Library/Application Support/Zotero/Profiles/d9rq1atq.default/zotero/storage/8QGMSGE9/Kerker et al_1982_Is the central dogma of flow cytometry true.pdf:application/pdf}
}
Downloads: 0
{"_id":"2rkp9pihbDRN6oTue","bibbaseid":"kerker-vandilla-brunsting-kratohvil-hsu-wang-gray-langlois-isthecentraldogmaofflowcytometrytruethatfluorescenceintensityisproportionaltocellulardyecontent-1982","downloads":0,"creationDate":"2016-12-25T11:56:59.501Z","title":"Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content?","author_short":["Kerker, M","Van Dilla, M a","Brunsting, A","Kratohvil, J P","Hsu, P","Wang, D S","Gray, J W","Langlois, R G"],"year":1982,"bibtype":"article","biburl":"http://plasmonics.github.io/zotero.bib","bibdata":{"bibtype":"article","type":"article","title":"Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content?","volume":"3","issn":"0196-4763","url":"http://doi.wiley.com/10.1002/cyto.990030202","doi":"10.1002/cyto.990030202","abstract":"Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 microns) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10% accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.","number":"2","journal":"Cytometry","author":[{"propositions":[],"lastnames":["Kerker"],"firstnames":["M"],"suffixes":[]},{"propositions":[],"lastnames":["Van","Dilla"],"firstnames":["M","a"],"suffixes":[]},{"propositions":[],"lastnames":["Brunsting"],"firstnames":["A"],"suffixes":[]},{"propositions":[],"lastnames":["Kratohvil"],"firstnames":["J","P"],"suffixes":[]},{"propositions":[],"lastnames":["Hsu"],"firstnames":["P"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["D","S"],"suffixes":[]},{"propositions":[],"lastnames":["Gray"],"firstnames":["J","W"],"suffixes":[]},{"propositions":[],"lastnames":["Langlois"],"firstnames":["R","G"],"suffixes":[]}],"year":"1982","pmid":"7140481","keywords":"Animals, Biophysical Phenomena, Biophysics, Cells, Flow cytometry, Fluorescent Dyes, microspheres, Particle Size","pages":"71--78","file":"Kerker et al_1982_Is the central dogma of flow cytometry true.pdf:/Users/baptiste/Library/Application Support/Zotero/Profiles/d9rq1atq.default/zotero/storage/8QGMSGE9/Kerker et al_1982_Is the central dogma of flow cytometry true.pdf:application/pdf","bibtex":"@article{kerker_is_1982,\n\ttitle = {Is the central dogma of flow cytometry true: {That} fluorescence intensity is proportional to cellular dye content?},\n\tvolume = {3},\n\tissn = {0196-4763},\n\turl = {http://doi.wiley.com/10.1002/cyto.990030202},\n\tdoi = {10.1002/cyto.990030202},\n\tabstract = {Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 microns) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10\\% accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.},\n\tnumber = {2},\n\tjournal = {Cytometry},\n\tauthor = {Kerker, M and Van Dilla, M a and Brunsting, A and Kratohvil, J P and Hsu, P and Wang, D S and Gray, J W and Langlois, R G},\n\tyear = {1982},\n\tpmid = {7140481},\n\tkeywords = {Animals, Biophysical Phenomena, Biophysics, Cells, Flow cytometry, Fluorescent Dyes, microspheres, Particle Size},\n\tpages = {71--78},\n\tfile = {Kerker et al_1982_Is the central dogma of flow cytometry true.pdf:/Users/baptiste/Library/Application Support/Zotero/Profiles/d9rq1atq.default/zotero/storage/8QGMSGE9/Kerker et al_1982_Is the central dogma of flow cytometry true.pdf:application/pdf}\n}\n\n","author_short":["Kerker, M","Van Dilla, M a","Brunsting, A","Kratohvil, J P","Hsu, P","Wang, D S","Gray, J W","Langlois, R G"],"key":"kerker_is_1982","id":"kerker_is_1982","bibbaseid":"kerker-vandilla-brunsting-kratohvil-hsu-wang-gray-langlois-isthecentraldogmaofflowcytometrytruethatfluorescenceintensityisproportionaltocellulardyecontent-1982","role":"author","urls":{"Paper":"http://doi.wiley.com/10.1002/cyto.990030202"},"keyword":["Animals","Biophysical Phenomena","Biophysics","Cells","Flow cytometry","Fluorescent Dyes","microspheres","Particle Size"],"downloads":0,"html":""},"search_terms":["central","dogma","flow","cytometry","true","fluorescence","intensity","proportional","cellular","dye","content","kerker","van dilla","brunsting","kratohvil","hsu","wang","gray","langlois"],"keywords":["animals","biophysical phenomena","biophysics","cells","flow cytometry","fluorescent dyes","microspheres","particle size"],"authorIDs":[],"dataSources":["RTrdwqnnisqkWbMNn"]}