Room temperature femtosecond X-ray diffraction of photosystem II microcrystals. Kern, J., Alonso-Mori, R., Hellmich, J., Tran, R., Hattne, J., Laksmono, H., Glöckner, C., Echols, N., Sierra, R. G., Sellberg, J., Lassalle-Kaiser, B., Gildea, R. J., Glatzel, P., Grosse-Kunstleve, R. W., Latimer, M. J., McQueen, T. A., DiFiore, D., Fry, A. R., Messerschmidt, M., Miahnahri, A., Schafer, D. W., Seibert, M. M., Sokaras, D., Weng, T., Zwart, P. H., White, W. E., Adams, P. D., Bogan, M. J., Boutet, S., Williams, G. J., Messinger, J., Sauter, N. K., Zouni, A., Bergmann, U., Yano, J., & Yachandra, V. K. Proceedings of the National Academy of Sciences, 109(25):9721–9726, June, 2012. Publisher: Proceedings of the National Academy of Sciences
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals [link]Paper  doi  abstract   bibtex   
Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This light-driven, four-photon reaction is catalyzed by the Mn4CaO5 cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However, the necessity for collecting data at room temperature, especially for studying the transient steps during the O–O bond formation, requires the development of new methodologies. In this paper we report room temperature X-ray diffraction data of PS II microcrystals obtained using ultrashort (\textless 50 fs) 9 keV X-ray pulses from a hard X-ray free electron laser, namely the Linac Coherent Light Source. The results presented here demonstrate that the ”probe before destroy” approach using an X-ray free electron laser works even for the highly-sensitive Mn4CaO5 cluster in PS II at room temperature. We show that these data are comparable to those obtained in synchrotron radiation studies as seen by the similarities in the overall structure of the helices, the protein subunits and the location of the various cofactors. This work is, therefore, an important step toward future studies for resolving the structure of the Mn4CaO5 cluster without any damage at room temperature, and of the reaction intermediates of PS II during O–O bond formation.
@article{kern_room_2012,
	title = {Room temperature femtosecond {X}-ray diffraction of photosystem {II} microcrystals},
	volume = {109},
	url = {https://www.pnas.org/doi/10.1073/pnas.1204598109},
	doi = {10.1073/pnas.1204598109},
	abstract = {Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This light-driven, four-photon reaction is catalyzed by the Mn4CaO5 cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However, the necessity for collecting data at room temperature, especially for studying the transient steps during the O–O bond formation, requires the development of new methodologies. In this paper we report room temperature X-ray diffraction data of PS II microcrystals obtained using ultrashort ({\textless} 50 fs) 9 keV X-ray pulses from a hard X-ray free electron laser, namely the Linac Coherent Light Source. The results presented here demonstrate that the ”probe before destroy” approach using an X-ray free electron laser works even for the highly-sensitive Mn4CaO5 cluster in PS II at room temperature. We show that these data are comparable to those obtained in synchrotron radiation studies as seen by the similarities in the overall structure of the helices, the protein subunits and the location of the various cofactors. This work is, therefore, an important step toward future studies for resolving the structure of the Mn4CaO5 cluster without any damage at room temperature, and of the reaction intermediates of PS II during O–O bond formation.},
	number = {25},
	urldate = {2024-12-10},
	journal = {Proceedings of the National Academy of Sciences},
	author = {Kern, Jan and Alonso-Mori, Roberto and Hellmich, Julia and Tran, Rosalie and Hattne, Johan and Laksmono, Hartawan and Glöckner, Carina and Echols, Nathaniel and Sierra, Raymond G. and Sellberg, Jonas and Lassalle-Kaiser, Benedikt and Gildea, Richard J. and Glatzel, Pieter and Grosse-Kunstleve, Ralf W. and Latimer, Matthew J. and McQueen, Trevor A. and DiFiore, Dörte and Fry, Alan R. and Messerschmidt, Marc and Miahnahri, Alan and Schafer, Donald W. and Seibert, M. Marvin and Sokaras, Dimosthenis and Weng, Tsu-Chien and Zwart, Petrus H. and White, William E. and Adams, Paul D. and Bogan, Michael J. and Boutet, Sébastien and Williams, Garth J. and Messinger, Johannes and Sauter, Nicholas K. and Zouni, Athina and Bergmann, Uwe and Yano, Junko and Yachandra, Vittal K.},
	month = jun,
	year = {2012},
	note = {Publisher: Proceedings of the National Academy of Sciences},
	pages = {9721--9726},
}
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