Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature

Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature. Kern, J., Alonso-Mori, R., Tran, R., Hattne, J., Gildea, R. J., Echols, N., Glöckner, C., Hellmich, J., Laksmono, H., Sierra, R. G., Lassalle-Kaiser, B., Koroidov, S., Lampe, A., Han, G., Gul, S., DiFiore, D., Milathianaki, D., Fry, A. R., Miahnahri, A., Schafer, D. W., Messerschmidt, M., Seibert, M. M., Koglin, J. E., Sokaras, D., Weng, T., Sellberg, J., Latimer, M. J., Grosse-Kunstleve, R. W., Zwart, P. H., White, W. E., Glatzel, P., Adams, P. D., Bogan, M. J., Williams, G. J., Boutet, S., Messinger, J., Zouni, A., Sauter, N. K., Yachandra, V. K., Bergmann, U., & Yano, J. Science, 340(6131):491–495, April, 2013. Publisher: American Association for the Advancement of Science
$Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature [link]$ Paper doi abstract bibtex

Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.

@article{kern_simultaneous_2013,
	title = {Simultaneous {Femtosecond} {X}-ray {Spectroscopy} and {Diffraction} of {Photosystem} {II} at {Room} {Temperature}},
	volume = {340},
	url = {https://www.science.org/doi/10.1126/science.1234273},
	doi = {10.1126/science.1234273},
	abstract = {Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.},
	number = {6131},
	urldate = {2024-12-10},
	journal = {Science},
	author = {Kern, Jan and Alonso-Mori, Roberto and Tran, Rosalie and Hattne, Johan and Gildea, Richard J. and Echols, Nathaniel and Glöckner, Carina and Hellmich, Julia and Laksmono, Hartawan and Sierra, Raymond G. and Lassalle-Kaiser, Benedikt and Koroidov, Sergey and Lampe, Alyssa and Han, Guangye and Gul, Sheraz and DiFiore, Dörte and Milathianaki, Despina and Fry, Alan R. and Miahnahri, Alan and Schafer, Donald W. and Messerschmidt, Marc and Seibert, M. Marvin and Koglin, Jason E. and Sokaras, Dimosthenis and Weng, Tsu-Chien and Sellberg, Jonas and Latimer, Matthew J. and Grosse-Kunstleve, Ralf W. and Zwart, Petrus H. and White, William E. and Glatzel, Pieter and Adams, Paul D. and Bogan, Michael J. and Williams, Garth J. and Boutet, Sébastien and Messinger, Johannes and Zouni, Athina and Sauter, Nicholas K. and Yachandra, Vittal K. and Bergmann, Uwe and Yano, Junko},
	month = apr,
	year = {2013},
	note = {Publisher: American Association for the Advancement of Science},
	pages = {491--495},
}

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